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Catalysts 2018, 8(1), 8; doi:10.3390/catal8010008

2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium

1
Department of Biochemistry and Molecular Biology I, Faculty of Biology, University Complutense of Madrid, José Antonio Nováis 12, E-28040 Madrid, Spain
2
Department of Crystallography and Structural Biology, Institute Rocasolano (CSIC), Serrano 119, E-28006 Madrid, Spain
These two researchers share the position of last author.
Present address: Ingenasa, Hermanos García Noblejas 39, E-28037 Madrid, Spain.
§
Present address: Applied Biotechnology Group, European University of Madrid, Tajo s/n, Villaviciosa de Odón, E-28670 Madrid, Spain.
*
Authors to whom correspondence should be addressed.
Received: 30 November 2017 / Revised: 26 December 2017 / Accepted: 29 December 2017 / Published: 3 January 2018
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Structure-function relationships of a novel 2′-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteristic of cold-adapted enzymes such as localized flexibility in critical loops, its biochemical properties are typical of mesophilic enzymes. BpNDT is a highly symmetrical homohexamer with tightly associated subunits that possesses flexible and short loops bordering the active sites. The catalytic center is essentially identical to that of other mesophilic homologues. Moreover, BpNDT shows that it is a mesophilic-like enzyme since it is not heat-labile and exhibits an apparent unfolding temperature (Tm) of 49 °C, being active during 96 h at 40 and 50 °C. Finally, BpNDT synthesizes natural and modified nucleosides, with preference for purines as acceptors and pyrimidine nucleosides as donors. Remarkably, the synthesis of several therapeutic nucleosides has been efficiently carried out. In this sense, 5-hydroxymethyl-2′-deoxyuridine (5-HMdUrd), 7-deaza-6-hydroxypurine-2′-deoxyriboside (7-DHPdRib) and theophylline-2′-deoxyriboside were synthesized for the first time by an NDT enzyme, showing the biotechnological interest of BpNDT. View Full-Text
Keywords: 2′-deoxyribosyltransferase; enzymatic synthesis; oligomeric assembly; protein crystallography; nucleoside analogues; therapeutic nucleosides 2′-deoxyribosyltransferase; enzymatic synthesis; oligomeric assembly; protein crystallography; nucleoside analogues; therapeutic nucleosides
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MDPI and ACS Style

Fresco-Taboada, A.; Fernández-Lucas, J.; Acebal, C.; Arroyo, M.; Ramón, F.; de la Mata, I.; Mancheño, J.M. 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium. Catalysts 2018, 8, 8.

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