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Catalysts 2017, 7(8), 238; doi:10.3390/catal7080238

Enzymatic Synthesis of S-Adenosylmethionine Using Immobilized Methionine Adenosyltransferase Variants on the 50-mM Scale

School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China
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Received: 17 July 2017 / Revised: 13 August 2017 / Accepted: 14 August 2017 / Published: 17 August 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

S-adenosylmethionine (SAM), an important metabolite in all living organisms, has been widely used to treat various diseases. To develop a simple and efficient method to produce SAM, an engineered variant of the methionine adenosyltransferase (MAT) from Escherichia coli was investigated for its potential use in the enzymatic synthesis of SAM due to its significantly decreased product inhibition. The recombinant I303V MAT variant was successfully produced at a high level (~800 mg/L) with approximately four-fold higher specific activity than the wild-type MAT. The recombinant I303V MAT was covalently immobilized onto the amino resin and epoxy resin in order to obtain a robust biocatalyst to be used in industrial bioreactors. The immobilized preparation using amino resin exhibited the highest activity coupling yield (~84%), compared with approximately 3% for epoxy resin. The immobilized enzyme was more stable than the soluble enzyme under the reactive conditions, with a half-life of 229.5 h at 37 °C. The KmATP value (0.18 mM) of the immobilized enzyme was ca. two-fold lower than that of the soluble enzyme. Furthermore, the immobilized enzyme showed high operational stability during 10 consecutive 8 h batches, with the substrate adenosine triphosphate (ATP) conversion rate above 95% on the 50-mM scale. View Full-Text
Keywords: S-adenosylmethionine; methionine adenosyltransferase; immobilized enzyme; enzyme variant; product inhibition S-adenosylmethionine; methionine adenosyltransferase; immobilized enzyme; enzyme variant; product inhibition
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MDPI and ACS Style

Niu, W.; Cao, S.; Yang, M.; Xu, L. Enzymatic Synthesis of S-Adenosylmethionine Using Immobilized Methionine Adenosyltransferase Variants on the 50-mM Scale. Catalysts 2017, 7, 238.

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