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Catalysts 2017, 7(5), 147; doi:10.3390/catal7050147

Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization

1
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland
2
Institute of Chemical and Environment Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, Pulaskiego 10, PL-70322 Szczecin, Poland
3
Department of Biochemistry, Duke University, 307 Research Drive, Durham, NC 27710, USA
4
Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Str. 23, Freiberg 09599, Germany
*
Authors to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 31 March 2017 / Revised: 30 April 2017 / Accepted: 5 May 2017 / Published: 10 May 2017
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Abstract

The main purpose of the study was to achieve effective immobilization of lipase B from Candida antarctica (CALB) onto 3D spongin-based scaffolds from Hippospongia communis marine demosponge for rapeseed oil transesterification. Successful immobilization onto the marine sponge skeleton was confirmed for the first time. Lipase B-containing biocatalytic system exhibited the highest catalytic activity retention (89%) after 60 min of immobilization at pH 7 and temperature of 4 °C. Immobilization was found to improve the thermal and chemical stability compared to free lipase, and retain over 80% of its initial catalytic activity over a wide range of temperature (30–60 °C) and pH (6–9). Additionally, immobilized lipase has good storage stability and retains over 70% of its initial activity even after catalyzing of 25 reaction cycles. The obtained product was used in a transesterification reaction of rapeseed oil with methanol and proved to be an efficient biocatalyst for biofuel production. The highest conversion value and fatty acids methyl esters (FAME) concentration were observed after a process conducted at 40 °C and pH 10. The possible mechanism of interaction between the enzyme and the spongin-based support is proposed and discussed. View Full-Text
Keywords: Hippospongia communis scaffolds; lipase B from Candida antarctica; lipase immobilization; immobilized lipase stability and reusability; rapeseed oil transesterification Hippospongia communis scaffolds; lipase B from Candida antarctica; lipase immobilization; immobilized lipase stability and reusability; rapeseed oil transesterification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zdarta, J.; Norman, M.; Smułek, W.; Moszyński, D.; Kaczorek, E.; Stelling, A.L.; Ehrlich, H.; Jesionowski, T. Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization. Catalysts 2017, 7, 147.

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