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Catalysts 2016, 6(8), 115; doi:10.3390/catal6080115

Covalent Immobilization of Candida rugosa Lipase at Alkaline pH and Their Application in the Regioselective Deprotection of Per-O-acetylated Thymidine

Departamento de Biocatálisis, Instituto de Catálisis (CSIC), Madrid 28049, Spain
Current address: Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, Argentina
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Author to whom correspondence should be addressed.
Academic Editor: Keith Hohn
Received: 10 June 2016 / Revised: 15 July 2016 / Accepted: 27 July 2016 / Published: 2 August 2016
(This article belongs to the Special Issue Asymmetric and Selective Biocatalysis)
View Full-Text   |   Download PDF [3441 KB, uploaded 2 August 2016]   |  

Abstract

Lipase from Candida rugosa (CRL) was stabilized at alkaline pH to overcome the inactivation problem and was immobilized for the first time by multipoint covalent attachment on different aldehyde-activated matrices. PEG was used as a stabilizing agent on the activity of CRL. At these conditions, CRL maintained 50% activity at pH 10 after 17 h incubation in the presence of 40% (w/v) of PEG, whereas the enzyme without additive was instantaneously inactive after incubation at pH 10. Thus, this enzyme was covalently immobilized at alkaline pH on three aldehyde-activated supports: aldehyde-activated Sepharose, aldehyde-activated Lewatit105 and heterofunctional aldehyde-activated EDA-Sepharose in high overall yields. Heterogeneous stable CRL catalysts at high temperature and solvent were obtained. The aldehyde-activated Sepharose-CRL preparation maintained 70% activity at 50 °C or 30% (v/v) acetonitrile after 22 h and exhibited high regioselectivity in the deprotection process of per-O-acetylated thymidine, producing the 3′-OH-5′-OAc-thymidine in 91% yield at pH 5. View Full-Text
Keywords: Candida rugosa lipase; stabilization; covalent immobilization; PEG; alkaline pH; regioselectivity; nucleosides Candida rugosa lipase; stabilization; covalent immobilization; PEG; alkaline pH; regioselectivity; nucleosides
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rivero, C.W.; Palomo, J.M. Covalent Immobilization of Candida rugosa Lipase at Alkaline pH and Their Application in the Regioselective Deprotection of Per-O-acetylated Thymidine. Catalysts 2016, 6, 115.

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