Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells
AbstractThe ubiquitous protein kinase CK2 has been demonstrated to be overexpressed in a number of human tumours. This enzyme is composed of two catalytic α or α’ subunits and a dimer of β regulatory subunits whose expression levels are probably implicated in CK2 regulation. Several recent papers reported that unbalanced expression of CK2 subunits is sufficient to drive epithelial to mesenchymal transition, a process involved in cancer invasion and metastasis. Herein, through transcriptomic and miRNA analysis together with comparison of cellular properties between wild type and CK2β-knock-down MCF10A cells, we show that down-regulation of CK2β subunit in mammary epithelial cells induces the acquisition of stem cell-like properties associated with perturbed polarity, CD44high/CD24low antigenic phenotype and the ability to grow under anchorage-independent conditions. These data demonstrate that a CK2β level establishes a critical cell fate threshold in the control of epithelial cell plasticity. Thus, this regulatory subunit functions as a nodal protein to maintain an epithelial phenotype and its depletion drives breast cell stemness. View Full-Text
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Duchemin-Pelletier, E.; Baulard, M.; Spreux, E.; Prioux, M.; Burute, M.; Mograbi, B.; Guyon, L.; Théry, M.; Cochet, C.; Filhol, O. Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells. Cancers 2017, 9, 114.
Duchemin-Pelletier E, Baulard M, Spreux E, Prioux M, Burute M, Mograbi B, Guyon L, Théry M, Cochet C, Filhol O. Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells. Cancers. 2017; 9(9):114.Chicago/Turabian Style
Duchemin-Pelletier, Eve; Baulard, Megghane; Spreux, Elodie; Prioux, Magali; Burute, Mithila; Mograbi, Baharia; Guyon, Laurent; Théry, Manuel; Cochet, Claude; Filhol, Odile. 2017. "Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells." Cancers 9, no. 9: 114.
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