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Cancers 2017, 9(9), 114; https://doi.org/10.3390/cancers9090114

Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells

1
Chemistry and Biology Department, Université Grenoble Alpes, F-38400 Grenoble, France
2
Biology of Cancer and Infection, UMRS1036, Inserm, F-38054 Grenoble, France
3
Biology of Cancer and Infection, Biosciences & Biotechnology Institute of Grenoble, CEA, F-38054 Grenoble, France
4
Laboratoire de Physiologie Cellulaire et Végétale, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS, F-38054 Grenoble, France
5
Biology Department, Inserm, CNRS, IRCAN, Université Côte d’Azur, F-06000 Nice, France
*
Author to whom correspondence should be addressed.
Academic Editor: Joëlle Roche
Received: 21 August 2017 / Revised: 21 August 2017 / Accepted: 28 August 2017 / Published: 31 August 2017
(This article belongs to the Special Issue The Epithelial-to-Mesenchymal Transition (EMT) in Cancer)
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Abstract

The ubiquitous protein kinase CK2 has been demonstrated to be overexpressed in a number of human tumours. This enzyme is composed of two catalytic α or α’ subunits and a dimer of β regulatory subunits whose expression levels are probably implicated in CK2 regulation. Several recent papers reported that unbalanced expression of CK2 subunits is sufficient to drive epithelial to mesenchymal transition, a process involved in cancer invasion and metastasis. Herein, through transcriptomic and miRNA analysis together with comparison of cellular properties between wild type and CK2β-knock-down MCF10A cells, we show that down-regulation of CK2β subunit in mammary epithelial cells induces the acquisition of stem cell-like properties associated with perturbed polarity, CD44high/CD24low antigenic phenotype and the ability to grow under anchorage-independent conditions. These data demonstrate that a CK2β level establishes a critical cell fate threshold in the control of epithelial cell plasticity. Thus, this regulatory subunit functions as a nodal protein to maintain an epithelial phenotype and its depletion drives breast cell stemness. View Full-Text
Keywords: protein kinase CK2; stem cell; breast cancer; EMT; epithelial plasticity protein kinase CK2; stem cell; breast cancer; EMT; epithelial plasticity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Duchemin-Pelletier, E.; Baulard, M.; Spreux, E.; Prioux, M.; Burute, M.; Mograbi, B.; Guyon, L.; Théry, M.; Cochet, C.; Filhol, O. Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells. Cancers 2017, 9, 114.

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