Next Article in Journal
Gene Regulatory Scenarios of Primary 1,25-Dihydroxyvitamin D3 Target Genes in a Human Myeloid Leukemia Cell Line
Next Article in Special Issue
Heterogeneity of Mesenchymal Markers Expression—Molecular Profiles of Cancer Cells Disseminated by Lymphatic and Hematogenous Routes in Breast Cancer
Previous Article in Journal
Three-Times Daily Ultrafractionated Radiation Therapy, A Novel and Promising Regimen for Glioblastoma Patients
Cancers 2013, 5(4), 1212-1220; doi:10.3390/cancers5041212
Review

Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR

,
,
,
 and *
Received: 10 July 2013; in revised form: 20 August 2013 / Accepted: 11 September 2013 / Published: 25 September 2013
(This article belongs to the Special Issue Circulating Tumor Cells in Cancers)
View Full-Text   |   Download PDF [344 KB, uploaded 25 September 2013]
Abstract: Breast cancer is still the most frequent cause of cancer-related death in women worldwide. Often death is not caused only by the primary tumour itself, but also by metastatic lesions. Today it is largely accepted, that these remote metastases arise out of cells, which detach from the primary tumour, enter circulation, settle down at secondary sites in the body and are called Circulating Tumour Cells (CTCs). The occurrence of such minimal residual diseases in the blood of breast cancer patients is mostly linked to a worse prognosis for therapy outcome and overall survival. Due to their very low frequency, the detection of CTCs is, still a technical challenge. RT-qPCR as a highly sensitive method could be an approach for CTC-detection from peripheral blood of breast cancer patients. This assumption is based on the fact that CTCs are of epithelial origin and therefore express a different gene panel than surrounding blood cells. For the technical approach it is necessary to identify appropriate marker genes and to correlate their gene expression levels to the number of tumour cells within a sample in an in vitro approach. After that, samples from adjuvant and metastatic patients can be analysed. This approach may lead to new concepts in diagnosis and treatment.
Keywords: circulating tumour cell (CTC); breast cancer; cytokeratin; marker gene; reverse transcriptase; qPCR circulating tumour cell (CTC); breast cancer; cytokeratin; marker gene; reverse transcriptase; qPCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Andergassen, U.; Kölbl, A.C.; Hutter, S.; Friese, K.; Jeschke, U. Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR. Cancers 2013, 5, 1212-1220.

AMA Style

Andergassen U, Kölbl AC, Hutter S, Friese K, Jeschke U. Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR. Cancers. 2013; 5(4):1212-1220.

Chicago/Turabian Style

Andergassen, Ulrich; Kölbl, Alexandra C.; Hutter, Stefan; Friese, Klaus; Jeschke, Udo. 2013. "Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR." Cancers 5, no. 4: 1212-1220.


Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert