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Cancers 2013, 5(3), 1163-1176; doi:10.3390/cancers5031163
Published: 10 September 2013
Abstract: Among gynecological malignancies epithelial ovarian cancer (EOC) is the leading cause of death. Despite improvements in conventional chemotherapy combinations, the overall cure rate has remained mostly stable over the years, and only 10%–15% of patients maintain a complete response following first-line therapy. To improve the efficacy of ovarian cancer chemotherapy it is essential to develop drugs with new mechanisms of action. Compared to normal tissues, protein disulfide isomerase (PDI) is overexpressed in ovarian tumors. PDI is a cellular enzyme in the lumen of the endoplasmic reticulum (ER) of eukaryotes or the periplasmic region of prokaryotes. This protein catalyzes the formation and breakage of disulphide bonds between cysteine residues in proteins, which affects protein folding. Selective inhibition of PDI activity has been exhibited both in vitro and in vivo anticancer activity in human ovarian cancer models. PDI inhibition caused accumulation of unfolded or misfolded proteins, which led to ER stress and the unfolded protein response (UPR), and in turn resulted in cell death. Nitazoxanide [NTZ: 2-acetyloxy-N-(5-nitro-2-thiazolyl)benzamide] is a thiazolide antiparasitic agent with excellent activity against a wide variety of protozoa and helminths. In this article, we propose that NTZ, acting as PDI inhibitor, may be a new and potent addition to the chemotherapeutic strategy against ovarian cancer.
Epithelial ovarian cancer (EOC) is responsible for 4% of cancer-deaths in women and continues to be the leading cause of death from gynecologic malignancies in the United States . The incidence of ovarian cancer in postmenopausal women is 5–6 times greater, and the mortality rate 10 times higher, than in premenopausal women . Although a family history of ovarian cancer is the most predictive risk factor for the development of the disease, only 5%–10% of ovarian cancers are inherited. In sporadic ovarian cancer, prolonged or incessant ovulation is thought to play a role in the etiology of the disease . Chronic inflammation can predispose a cell to ovarian carcinoma. The inflammatory pathway that occurs during incessant ovulation precipitates increased risk for EOC . Cytokines and other proinflammatory mediators contribute to both extrinsic and intrinsic pathways of inflammation-associated carcinogenesis. Among these mediating, proinflammatory cytokine IL-6 is involved in the development and progression of EOC.
Primary treatment of ovarian and ovarian-related cancers should consist of optimal cytoreduction. After surgery, the standard treatment is a combination of platinum and taxane chemotherapy. Unfortunately, the majority of patients (up to 70%) who present with advanced stages of disease exhibit recurrent or persistent disease following primary treatment. Despite advances in surgical techniques and chemotherapeutic agents, mortality rates from EOC have decreased only slightly over the last 30 years .
Drug resistance is thought to cause treatment failure and death in more than 90% of patients with metastatic disease . Given the high incidence of chemo-resistant ovarian cancers and the poor prognosis, continued research efforts focus on the development of novel treatments. Particularly pharmacologic strategies that may circumvent the defective apoptotic cell death pathway that characterizes tumor cells in recurrent disease.
Recently, propionic acid carbamoyl methyl amides (PACMAs) have shown a broad spectrum of cytotoxicity in a panel of human cancer cell lines, with relatively selective potency in ovarian cancer cells resistant to doxorubicin and paclitaxel . PACMAs demonstrated in vitro and in vivo anticancer activity by targeting protein disulfide isomerase (PDI), a superfamily of oxidoreductase proteins localized in the endoplasmic reticulum (ER), nucleus, cytosol, mitochondria and cell membrane.
Currently, the PDI gene family contains 21 members, varying in domain composition, molecular weight, tissue expression, and cellular processing. PDI is the most abundant chaperone/isomerase in the endoplasmic reticulum and plays pivotal role in protein folding through isomerase and chaperone activity. Given their vital role in protein-folding, loss of PDI activity has been associated with the pathogenesis of numerous diseases, most commonly related to the unfolded protein response (UPR) including malignancy. During tumor development, PDI is involved in the ER stress response that allows cancer cells to survive outside their normal environment. Increased PDI levels have been documented in a variety of human cancers, including ovarian , prostate , glioma , acute myeloid leukemia , and melanoma .
The activation of cellular stress responses, mediated by the ER, promotes survival of cancer cells . UPR reduces new translation of protein and triggers the degradation of unfolded and aggregated proteins. Ultimately, if protein homeostasis mechanisms are insufficient to protect or repair the cell, ER stress will induce programmed cell death . The cell will cope in one of two ways after experiencing ER stress; it will either adapt and survive or, alternatively, set in motion apoptotic/senescence programing.
In a study conducted by Lovat et al., the antibiotic bacitracin was shown to increase cell death of melanoma cells; cell death was a consequence of ER stress . Bacitracin is a dodecapeptide antibiotic produced by certain strains of Bacillus licheniformis and Bacillus subtilis. This antibiotic’s ability to inhibit PDI activity led to the effects it had on melanoma cells . However, due to nephrotoxicity and low membrane permeability, clinical use of bacitracin is limited.
Unlike the antibiotic mentioned above, nitazoxanide (NTZ)—of the thiazolide family—is a safe and inexpensive broad-spectrum, FDA approved, drug. NTZ is traditionally utilized for the treatment of anaerobic intestinal parasites Giardia lamblia and Cryptosporidium parvum, and has also been found efficacious in the treatment of other anaerobic bacteria and parasites residing in the human bowel . Studies of protozoa and anaerobic bacteria have shown that NTZ inhibits pyruvate-ferredoxin oxidoreductase (PFOR), an essential enzyme for anaerobic energy metabolism . The pharmacological effects of NTZ are not restricted to antiparasitic activities. In fact, NTZ has also been successfully used to promote HCV elimination by improving interferon signaling and promoting autophagy . Similarly, the ability to inhibit PDI has been attributed to NTZ as well .
Although NTZ was initially designed as an anti-microbial drug, anti-cancer properties have also been observed. A recent study accessing the development of drugs regulating c-Myc validated NTZ’s anti-Myc activity . Another previous study established that NTZ and the bromothiazolide RM4819 can inhibit the proliferation of colon cancer cells in vitro when bound to the protein glutathione S-transferase P1-1 (GSTP1) . A corresponding report stated that there is a correlation between the expression of GSTP1 proteins in tumor tissues and prognosis in ovarian cancer . Specifically, high levels of GSTP1 in tumor cells may greatly limit the efficacy of antitumor chemotherapy. The GSSG/2GSH redox couple is considered to be the major thiol-disulfide redox buffer of the cell. It has also previously been reported that PDI-dependent refolding/isomerization is optimal at a GSH:GSSG ratio of 5:1 .
The purpose of this article is to propose, for the first time, the intriguing hypothesis that NTZ may sensitize cancer cells to stress-induced cell demise by blocking the PDI that under typical physiological circumstances restores ER homeostasis. In this fashion, we suggest that NTZ might enhance the efficacy of standard chemotherapy regiments in ovarian cancer patients and ultimately improve survival.
2. Unfolded Protein Response in Tumor Development: The Role of PDI
The endoplasmic reticulum (ER) is the first compartment of the secretory pathway. It plays a major role in ER chaperone-assisted folding and quality control, including post-translational modification like the disulfide bond formation of newly synthesized secretory proteins . Protein folding and assembly takes place in the ER, where redox conditions are distinctively different from the other organelles, and are favorable for disulfide formation . For the biosynthesis and the function of many proteins, disulfide bonds are crucial. They promote structural stability, facilitate the assembly of multi-protein complexes and can modulate redox-dependent functions in response to changes in the cell. Disulfide bonds can also mediate the formation of productive folding intermediates and have been shown in the ER to promote thiol-mediated protein retention .
ER stress can be provoked through a variety of physiological conditions, including perturbations in calcium bhomeostasis, glucose/energy deprivation, redox changes, ischemia, hyperhomocystinemia, viral infections and mutations that impair client protein folding . Likewise, an inadequate supply of glucose affects protein glycosylation and the production of ATP, both of which could lead to the accumulation of unfolded proteins in the ER, resulting in ER stress. Elevated ER stress and increased chaperone protein expression have been correlated with tumor initiation, malignancy and metastasis . Cancer cells face an exceptionally harsh microenvironment during their growth, including hypoxia, nutrient deprivation, acidosis, and non-permissive interactions with stromal cells and extracellular matrix. During tumor development, several ER stress activators (hypoxia and low glucose) are also known to induce resistance to chemotherapy through processes that are suggestive of ER-dependent mechanisms .
As a consequence of ER stress, the cell has evolved an adaptive, coordinated response to limit accumulation of unfolded proteins in the ER. This signaling pathway is termed the unfolded protein response (UPR) . The molecular chaperones and folding sensors in the ER include the glucose regulated proteins 78 (GRP78; also known as Immunoglobulin Binding protein—BiP) and 94 (GRP94), the lectins, calnexin and calreticulin, and the thiol-disulfide oxidoreductases, protein disulfide isomerase (PDI) . Studies on the role of PDI in cancer have demonstrated a pro-oncogenic, pro-survival function for PDI in cancer and therapeutic resistance .
Mammalian PDI is a multifunctional protein with both enzymatic oxidoreductase and chaperone properties . PDI catalyzes native disulfide bond formation through thiol-disulfide oxidation, reduction and isomerization and is essential to protein folding and quality control in the ER. The UPR adaptive response aims to reestablishing homeostatic cellular equilibrium. Establishing equilibrium includes up-regulation of molecular chaperones and protein-processing enzymes to increase protein folding, reduce ER workload, and prevent further accumulation of unfolded proteins .
The physiological responses ultimately regulated by the UPR are induction of molecular chaperones and upregulation of ER-associated Protein Degradation (ERAD). ERAD is responsible for the degradation of aberrant or misfolded proteins, which are potentially toxic for the cell . In fact, a protein that fails to achieve the correct conformation in the ER exits the folding cycle and is targeted for degradation.
If ERAD fails to promote clearance of unwanted proteins while the cell is under extreme ER stress, cell death may be triggered. This process involves caspase-dependent apoptosis and caspase-independent death . Mounting evidence suggests that ER stress-induced apoptosis contributes to a range of human diseases that involve cell loss, including diabetes , neurodegeneration , and stroke . The contribution of ER stress in these distinct diseases varies depending on the cell type affected and the intracellular and/or extracellular conditions .
Although apoptosis is the primary form of programmed cell death, there are some instances wherein cell death is associated with the presence of autophagic vacuoles. These are vacuoles indicative of autophagic cell death. Autophagy mediated cell death is purportedly linked to the apoptotic pathway through alterations in mitochondrial function. Both apoptotic and autophagic pathways are reported to share mediators, which supports the thesis that there is crosstalk between them . Autophagy, literally meaning “self-eating”, involves the engulfment of proteins and entire organelles within double membrane vesicles called autophagosomes. Autophagy plays a housekeeping role in protein degradation (complementing the proteasome-based protein degradation system) and acts in the catabolic cellular process, during times of nutrient deprivation . Furthermore, it has been documented that some cells also display autophagosome-like structures containing ER derived membrane stacks after prolonged UPR activation . Potentially toxic aggregates are segregated into ER subdomains and are subsequently removed by autophagy.
3. Autophagy and Cancer
Autophagy is instrumental in a variety of physiological and pathophysiological processes, including cellular homeostasis, survival, development, and differentiation. Autophagy accomplishes the degradation and recycling of redundant and aged molecules and organelles, and destroys potentially harmful molecular and cellular components. For these reasons, autophagy has consistently been observed in response to ER stress as a complementary mechanism to the UPR whose function is to alleviate protein stress (Figure 1).
Autophagy is ongoing at basal levels in eukaryotic cells and allows the cell to function optimally by removing unwanted substrates which may otherwise lead to cellular toxicity . Eukaryotic cells are continuously exposed to environmental perturbation, which inflict minor stresses on the cell and disrupt its homeostatic environment. The fluctuations in the cell’s environment can result in the accumulation of misfolded protein aggregates, reactive oxygen species (ROS), and damaged organelles. Selective autophagy evidences the possibility of selective degradation of mitochondria (mitophagy), ER (ER phagy/reticulophagy) , ribosomes (ribophagy), peroxisomes (pexophagy), Golgi (crinophagy), endosomes (heterophagy), pathogens (xenophagy)  and lipids (lipophagy) . The role of autophagy as a tumor suppressor implies its ability to remove damaged organelles, growth factors, and face chromosomal instability . Autophagy is regulated via a group of genes called AuTophaGy related Genes (ATGs) and is executed at basal levels in virtually all cells as a homeostatic mechanism for maintaining cellular integrity .
Genetic evidence for tumor suppressing function has been demonstrated in the autophagy-related gene Beclin1 . Beclin1 was the first oncosuppressor gene linked to autophagy and cancerogenesis . Beclin1, the mammalian orthologue to yeast ATG6, was identified due to its interaction with Bcl-2. Bcl-2 binds to beclin1 and inhibits its autophagic activity by blocking its interaction with Vps34 . A genetic study revealed that mice, homozygously deleted for beclin1, die during embryogenesis . The same study also demonstrated that heterozygous disruption of beclin1 promoted tumorigenesis in mice . Mice lacking a single copy of beclin1 (beclin +/−) developed spontaneous tumors including lymphoma, hepatocellular carcinoma, lung adenocarcinomas, and mammary hyperplasia.
High grade ovarian cancer cells express very low levels of the autophagy protein LC3, indicating that LC3-labeled autophagosomes do not accumulate. Moreover, the impairment of autophagic function has been correlated with alteration of the oncosuppressors PTEN, ARHI and p53 in ovarian cancer cells. For instance, deficient p53 mutants are unable to sequester bcl-2 which inhibits the formation of the autophagy interactome by interacting with beclin1 . Over-expression of mutated p53 in ovarian cancer cells may indirectly impact autophagy. Conversely, hyper-expression of both beclin1 and LC3 in ovarian cancer cells is associated with favorable chemotherapeutic response in patients.
Other indirect evidence that autophagy may act as a tumor-suppressant can be observed in the instigation of the PI3K/Akt pathway via PI3K mutations, AKT amplifications, or PTEN loss, which leads to decreased autophagy in many settings, largely through mTOR activation [44,45]. TOR is a protein kinase central to the regulation of cell growth. It plays a key role in the balance between cell growth and autophagy when responding to nutritional status, growth factor and stress signals.
mTOR negatively regulates autophagy by causing phosphorylation of Atg13, which reduces its interaction with ULK1 and inhibits formation of a trimeric complex required for autophagosome formation . The fact that aberrant mTORC1 signaling is observed in 40%–90% of human cancers makes the investigation of the role of autophagy in cancer intriguing . We have yet to determine whether autophagy is behind tumor suppression or tumor progression and critical questions remain regarding this dual, dynamic role of autophagy in cancer. However in this context, the cytocidial role of autophagy is particularly significant.
4. Autophagy and Cell Death
In addition to the cytoprotective function of autophagy, it has been proposed that autophagy is a mechanism for cell death. This conclusion was drawn after morphological features of autophagy have been observed within dying cells.
Programed cell death (PCD) is an evolutionarily conserved essential for several vital functions, including developmental morphogenesis, tissue homeostasis, and defense against pathogens . Apoptosis, literally “dropping off,” is a tightly regulated and efficient cell death program which requires the interplay of several factors. Morphological hallmarks of apoptosis in the nucleus are chromatin condensation and nuclear fragmentation, accompanied by rounding up of the cell, reduction in cellular volume (pyknosis) and retraction of pseudopodes . In human cancer apoptotic pathways are frequently disabled . This capacity to evade cell death allows for the malignant transformation of cancer cells and leads to cancer metastasis and cancer drug resistance.
PCD does not seem to be restricted to apoptosis. Another classification of cell demise was identified by Clark and is known as type II cell death. He observed morphological change such as multiple‐membrane enclosed vesicles in the cytoplasm that engulf portions of cytoplasm and/or organelles in cell death . It has been speculated that this phenomenon may reflect the existence of a programmed-autophagic, type II cell death or, alternatively, an adaptive response meant to maintain continual cell survival under stress conditions. Many reports show that autophagy is induced by ER stress . For instance, a panel of ovarian cancer cell lines treated with saquinavir, an antiretroviral protease inhibitor, experienced caspase-dependent apoptosis and caspase-independent cell death characterized by induction of ER stress and autophagy .
The antineoplastic effects of protease inhibitors include inhibition of AKT signaling, inhibition of angiogenesis and the provocation of autophagy following induction of ER stress. The authors draw the conclusion that ER stress and autophagy are important mechanisms of protease inhibitor-mediated cell death in ovarian cancer cells. Similarly, Bursch and colleagues reported that most MCF7 breast carcinoma cells undergo autophagic cell death, rather than apoptosis, after treatment with the anti-estrogen agent tamoxifen . Thus, it would appear that on one hand, autophagy may be protecting cells from poor nutrition or dysfunctional mitochondria, but on the other hand could be leading cells to their death by degrading essential components of the cytosol.
Indeed it is a reasonable conclusion that autophagic activity above a certain threshold may destroy a major portion of the cytosol and organelles, and in turn lead to an irreversible collapse of cellular functions. During extensive autophagy, the total area of the autophagic vacuoles and dense bodies are roughly equal to or greater than that of cytosol and organelles outside the vacuoles . In this manner, autophagy might be operating as a backup to ERAD, if degradative substrates dislocated into the cytosol overwhelm the ERAD capacity.
ER stress autophagy and death cell may be triggered by several pathways. Nevertheless it is not yet completely understood if cell death is a consequence of the severity and/or extended duration of autophagy or if it is a deliberate mechanism of programmed cell death such as apoptosis.
In our paper we postulate that the inhibition chaperone function of PDI, whose overexpression allows cancer cells to survive under harsh conditions, pushes the cells into an extreme autophagic process in order to cope with misfolded proteins and to relieve stress. Yet this prolonged and irreversible ER stress leads ultimately to cell death which is the main goal of any anti-cancer chemotherapy.
5. Thiazolides Compound: Old Drug, New Purpose?
Nitazoxanide (NTZ) is a main compound of a class of broad-spectrum anti-parasitic compounds named thiazolides. It is composed of a nitrothiazole-ring and a salicylic acid moiety which are linked together by an amide bond (Figure 2). NTZ was developed in 1975 as a veterinary antiparasitic agent by Jean Francois Rossginol . The US Food and Drug Administration (FDA) approved oral suspension NTZ in December of 2002 for the treatment of diarrhea caused by Cryptosporidium species and Giardia intestinalis in pediatric patients 1–11 years of age. In July 2004, NTZ was approved for treatment of diarrhea caused by G. intestinalis in adults. NTZ is generally well tolerated, and no significant adverse events have been noted in human trials .
In vitro, NTZ and tizoxanide function against a wide range of organisms, including the protozoal species Blastocystis hominis, C. parvum, Entamoeba histolytica, G. lamblia and Trichomonas vaginalis . In addition, the in vitro activity of anaerobic bacteria and rotavirus are also inhibited by NTZ. In regards to its antiparasitic activities, NTZ has been shown to interfere with the pyruvate ferredoxin oxidoreductase, an enzyme essential to the anaerobic energy metabolism of the parasites. These enzymes are found in the amitochondriate eukaryotic human parasites (Trichomonas vaginalis, Entamoeba histolytica, and Giardia intestinalis), including Cryptosporidium parvum, most anaerobic bacteria (Clostridium spp.), Archaea, and microaerophiles of the epsilon proteobacterial group . Other data report that NTZ inhibits HCV replication by a cell-mediated mechanism: either stimulating innate cell defense processes or inhibiting the maturation of key viral proteins . However, the mechanism by which this total process occurs has yet to be completely elucidated.
NTZ has exhibited considerable anti-Neospora caninum tachyzoite activity in vitro . Neospora caninum is an important intracellular protozoan parasite closely related to Toxoplasma gondii. Muller et al.’s work demonstrated, for the first time, that the activities of NTZ can be attributed to the inhibition of protein disulfide isomerase in the test parasite . Even more interesting however, appears to be the role of NTZ in preventing the replication of Mtb in human cells . Because PFOR is not known to be present in Mtb, the authors suggested an alternative mechanism might act within this process.
mTORC1 is a major negative regulator of autophagy, its inhibition by NTZ contributes to the prevention of M. tuberculosis proliferation in infected macrophages . NTZ structurally resembles niclosamide, an anthelmintic drug that stimulates autophagy and inhibits signaling by mTORC1. mTORC1 is also frequently deregulated in cancer and its inhibition is a promising strategy in the treatment of tumors.
NTZ has also shown significant immunomodulation properties, inhibiting lipopolysaccharide (LPS)-induced pro-inflammatory cytokine production in macrophages . Chronic inflammation is recognized as a common risk factor for various human cancers because it creates a pro-proliferative environment for tumor cells . Many studies have suggested that the development of cancer may be driven by inflammatory cells, as well as by a variety of mediators including cytokines, chemokines and enzymes .
Interleukin (IL-6) is a proinflammatory cytokine whose irregular production has been implicated in the development of various inflammatory and autoimmune diseases including cancer. IL-6 has had tumor-promoting effects on both malignant and stromal cells in a range of experimental cancer models. Specifically in ovarian cancer, IL-6 enhances tumor cell survival and increases resistance to chemotherapy via JAK/STAT signaling in tumor cells . IL-6 is highly expressed in the tumor context of type-2 ovarian cancers, particularly in ascitic fluid, and its level correlates with poor prognosis . In advanced EOC ascites, there are also an increased number of macrophages involved in cancer metastasis and progression as they modify the tumor microenvironment. Interestingly macrophage depletion, in peritoneal ovarian cancer models, suppresses cancer progression and the accumulation of ascites .
The first report of NTZ having direct inhibitory ability on the production of IL-6 was recently published in a non-oncological study evaluating in vitro and in vivo mice models with LPS-stimulated macrophages . The mechanism by which NTZ blocks the production of IL-6 from LPS-stimulated macrophages is currently up for speculation. It is plausible to suppose that autophagy may be partly responsible for the inhibition of IL-6 because of its ability to shape the inflammatory reaction. For instance, autophagic death could be beneficial in controlling the level and duration of inflammation seeing as necrotic cells are potent inducers of the inflammatory response in nearby immune competent cells. Therefore, the removal of dying cells as a part of the autophagic function might prevent undesirable oxidative stress, inflammatory response and thus the release of such proinflammatory cytokines.
From an oncological perspective, NTZ and select derivatives shown to induce apoptosis in colon cancer cells in vitro. The enzymatic activity of recombinant glutathione-S-transferase (GSTP1) was strongly inhibited by thiazolides when evaluated through affinity chromatography and mass spectrometry. High levels of GSTP1 in tumor cells may substantially limit the efficacy of antitumor chemotherapy, much like the PDIs discussed above especially in regards the similar and cross-related function between them.
In a very recent study, NTZ was observed to inhibit c-Myc. The c-Myc oncogene is overexpressed in the majority of human cancers and contributes to the cause of at least 40% of tumors. c-Myc drives cell proliferation and is critically involved in the regulation of many growth-promoting signal transduction pathways . In particular, c-Myc is involved in a complex inflammatory response which leads to the recruitment of various inflammatory cells with pro-tumorigenic behavior. c-Myc is amplified and overexpressed in serous cancers and inversely correlates with prognosis. In breast cancer xenograft mouse models, NTZ significantly suppressed tumor growth by inhibiting c-Myc and inducing apoptosis . These findings support NTZ’s potential as a new, anti-tumor agent for the inhibition of c-Myc associated neoplasia, including ovarian cancer.
In this concept paper we focused on PDI as an anti-cancer target for NTZ and aimed to draw organized connections between ER stress, URP and autophagy. Since our speculative area of interest is ovarian cancer, our hypothesis began with the finding that the anti-cancer properties of PACMAs worked by inhibiting the PDI in ovarian cancer cells. PDI is a dithiol-disulfide oxidoreductase chaperone from the thioredoxin superfamily and regulates a broad range of processes including protein folding, signal transduction, protein traffic, cell communication, and extracellular cell surface events related to thrombosis and immune functions. These regulatory behaviors converge to significantly affect cell redox status and regulate cell survival or death.
Once we recognized the broad spectrum of action that PDI has, we investigated the possibility that there might be drugs currently used in clinical practice that could mimic the anti-PDI effect of PACMAs. We determined, based on previous publications, that the antiparasitic drug NTZ may show anti-PDI activity similar to that of the PACMAs. We restricted our focus to antiparasitic agent on the basis of the theoretical assumption that cancer cells, during their development display some unicellular/prokaryotic behavior reminiscent of robust ancient life forms . The regression of cancer cells involves changes within metabolic machinery and survival strategies. Furthermore, we have noted that NTZ presented some important immunomodulatory properties specifically anti-IL-6 cytokine action. This property of NTZ could be particularly relevant in ovarian carcinoma where IL-6 is often overexpressed in many paclitaxel-resistant ovarian cancer cells.
However, we do not exclude that there could be other mechanisms of action behind PDI inhibition and the ER stress cascade pathways. Further studies evaluating the preclinical, antitumor properties of NTZ are imperative, especially for ovarian cancer which is among the top four most lethal female malignant diseases in United States. Nevertheless, we endorse the idea that NTZ might have anti-cancer activity toward other solid epithelial cancers. It is worth recognizing that NTZ is an effective, safe, orally bioavailable and inexpensive drug, already approved for human use. To the best of our knowledge, there has been no report about the relationship between NTZ and ovarian cancer treatment.
The decision to live or die marks cell fate. Understanding how cells make that decision is essential for the development an anti-neoplastic strategy meant to overcome current treatment failure. An increasing number of studies are supporting the role of PDI in the development of malignancy. PDI inhibition causes accumulation of unfolded or misfolded proteins that leads to ER stress. ER stress can switch the cytoprotective functions of UPR into a cell death promoting pathway. Cancer cells under extreme or prolonged ER stress, by way of autophagic cell death pathways, can execute a cellular demise.
Autophagy is an evolutionarily conserved, lysosomal-mediated catabolic response pathway that is induced by metabolic stress particularly under nutrient-poor conditions. During cancer development, growth factors should be signaling for stimulation of anabolism and suppression of catabolism because replicative cell division requires a doubling of biomass. This may also explain the down-regulation of autophagy in cancer cells. Hence, the PDI inhibitor strategy is becoming an interesting therapeutic approach to killing cancer cells, particularly in the context of impairing apoptosis pathways. In this context, we propose NTZ as a viable tool for the inhibition of PDI.
Ovarian carcinoma remains one of the most lethal female malignancies. A lack of available treatment against advance metastatic disease is responsible for the high mortality. Unfortunately, due to an increase in drug resistance, it is the therapies themselves that represent a major barrier to the long-term efficacy of the ovarian cancer treatment. The development of new cancer drugs is usually a slow and costly process, while new, more effective anti-cancer therapies are critical and urgent. During 2012, in the United States, approximately 22,000 women were diagnosed with EOC. In conclusion, future preclinical studies focusing on the ability of NTZ, either acting alone or as coadjuvant in the treatment of ovarian carcinoma, are compelling.
We thank our colleagues at the Duke University Medical Center Department of Gynecologic Oncology for their continued support.
Conflicts of Interest
The authors declare that there are no conflicts of interest.
- Siegel, R.; Naishadham, D.; Jemal, A. Cancer statistics. 2012. CA Cancer J. Clin. 2012, 62, 10–29, doi:10.3322/caac.20138.
- Kurman, R.J.; Shih, I.-M. The origin and pathogenesis of epithelial ovarian cancer: A proposed unifying theory. Am. J. Surg. Pathol. 2010, 34, 433–443, doi:10.1097/PAS.0b013e3181cf3d79.
- Maccio, A.; Madeddu, C. Inflammation and ovarian cancer. Cytokine 2012, 58, 133–147, doi:10.1016/j.cyto.2012.01.015.
- Petty, R.; Evans, A.; Duncan, I.; Kurbacher, C.; Cree, I. Drug resistance in ovarian cancer—The role of p53. Pathol. Oncol. Res. 1998, 4, 97–102, doi:10.1007/BF02904701.
- Xu, S.; Butkevich, A.N.; Yamada, R.; Zhou, Y.; Debnath, B.; Duncan, R.; Zandi, E.; Petasis, N.A.; Neamati, N. Discovery of an orally active small-molecule irreversible inhibitor of protein disulfide isomerase for ovarian cancer treatment. Proc. Natl. Acad. Sci. USA 2012, 109, 16348–16353, doi:10.1073/pnas.1205226109.
- Welsh, J.B.; Sapinoso, L.M.; Su, A.I.; Kern, S.G.; Wang-Rodriguez, J.; Moskaluk, C.A.; Frierson, H.F.; Hampton, G.M. Analysis of gene expression identifies candidate markers and pharmacological targets in prostate cancer. Cancer Res. 2001, 61, 5974–5978.
- Goplen, D.; Wang, J.; Enger, P.Ø.; Tysnes, B.B.; Terzis, A.J.A.; Laerum, O.D.; Bjerkvig, R. Protein disulfide isomerase expression is related to the invasive properties of malignant glioma. Cancer Res. 2006, 66, 9895–9902, doi:10.1158/0008-5472.CAN-05-4589.
- Haefliger, S.; Klebig, C.; Schaubitzer, K.; Schardt, J.; Timchenko, N.; Mueller, B.U.; Pabst, T. Protein disulfide isomerase blocks CEBPA translation and is up-regulated during the unfolded protein response in AML. Blood 2011, 117, 5931–5940.
- Lovat, P.E.; Corazzari, M.; Armstrong, J.L.; Martin, S.; Pagliarini, V.; Hill, D.; Brown, A.M.; Piacentini, M.; Birch-Machin, M.A.; Redfern, C.P.F. Increasing melanoma cell death using inhibitors of protein disulfide isomerases to abrogate survival responses to endoplasmic reticulum stress. Cancer Res. 2008, 68, 5363–5369, doi:10.1158/0008-5472.CAN-08-0035.
- Ma, Y.; Hendershot, L.M. The role of the unfolded protein response in tumour development: Friend or foe? Nat. Rev. Cancer 2004, 4, 966–977, doi:10.1038/nrc1505.
- Moenner, M.; Pluquet, O.; Bouchecareilh, M.; Chevet, E. Integrated endoplasmic reticulum stress responses in cancer. Cancer Res. 2007, 67, 10631–10634, doi:10.1158/0008-5472.CAN-07-1705.
- Dickerhof, N.; Kleffmann, T.; Jack, R.; McCormick, S. Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain. FEBS J. 2011, 278, 2034–2043, doi:10.1111/j.1742-4658.2011.08119.x.
- White, A.C., Jr. Nitazoxanide: A new broad spectrum antiparasitic agent. Expert Rev. Anti Infect. Ther. 2004, 2, 43–49, doi:10.1586/14787184.108.40.206.
- Theodos, C.M.; Griffiths, J.K.; D’Onfro, J.; Fairfield, A.; Tzipori, S. Efficacy of nitazoxanide againstcryptosporidium parvum in cell culture and in animal models. Antimicrob. Agents Chemother. 1998, 42, 1959–1965.
- Elazar, M.; Liu, M.; McKenna, S.A.; Liu, P.; Gehrig, E.A.; Puglisi, J.D.; Rossignol, J.F.; Glenn, J.S. The anti-hepatitis C agent nitazoxanide induces phosphorylation of eukaryotic initiation factor 2α via protein kinase activated by double-stranded RNA activation. Gastroenterology 2009, 137, 1827–1835, doi:10.1053/j.gastro.2009.07.056.
- Müller, J.; Naguleswaran, A.; Müller, N.; Hemphill, A. Neospora caninum: Functional inhibition of protein disulfide isomerase by the broad-spectrum anti-parasitic drug nitazoxanide and other thiazolides. Exp. Parasitol. 2008, 118, 80–88, doi:10.1016/j.exppara.2007.06.008.
- Fan-Minogue, H.; Bodapati, S.; Solow-Cordero, D.E.; Fan, A.; Paulmurugan, R.; Massoud, T.; Felsher, D.W.; Gambhir, S.S. A c-Myc activation sensor-based high throughput drug screening identifies an anti-neoplastic effect of nitazoxanide. Mol. Cancer Ther. 2013, doi:10.1158/1535-7163.MCT-12-1243.
- Müller, J.; Sidler, D.; Nachbur, U.; Wastling, J.; Brunner, T.; Hemphill, A. Thiazolides inhibit growth and induce glutathione-S-transferase Pi (GSTP1)-dependent cell death in human colon cancer cells. Int. J. Cancer 2008, 123, 1797–1806, doi:10.1002/ijc.23755.
- Hirazono, K.; Shinozuka, T.; Kuroshima, Y.; Itoh, H.; Kawai, K. Immunohistochemical expression of glutathione S-transferase Pi (GST-Pi) and chemotherapy response in malignant ovarian tumors. J. Obstet. Gynaecol. (Tokyo 1995) 1995, 21, 305–312, doi:10.1111/j.1447-0756.1995.tb01015.x.
- Lundstrom-Ljung, J.; Holmgren, A. Glutaredoxin accelerates glutathione-dependent folding of reduced ribonuclease a together with protein disulfide-isomerase. J. Biol. Chem. 1995, 270, 7822–7828.
- Feige, M.J.; Hendershot, L.M. Disulfide bonds in er protein folding and homeostasis. Curr. Opin. Cell Biol. 2011, 23, 167–175, doi:10.1016/j.ceb.2010.10.012.
- Gruber, C.W.; Čemažar, M.; Heras, B.; Martin, J.L.; Craik, D.J. Protein disulfide isomerase: The structure of oxidative folding. Trends Biochem. Sci. 2006, 31, 455–464, doi:10.1016/j.tibs.2006.06.001.
- Wu, J.; Kaufman, R. From acute ER stress to physiological roles of the unfolded protein response. Cell Death Differ. 2006, 13, 374–384, doi:10.1038/sj.cdd.4401840.
- Hughes, C.S.; Shen, J.W.; Subjeck, J.R. Resistance to etoposide induced by three glucose-regulated stresses in Chinese hamster ovary cells. Cancer Res. 1989, 49, 4452–4454.
- Feldman, D.E.; Chauhan, V.; Koong, A.C. The unfolded protein response: A novel component of the hypoxic stress response in tumors. Mol. Cancer Res. 2005, 3, 597–605, doi:10.1158/1541-7786.MCR-05-0221.
- Ko, H.S.; Uehara, T.; Nomura, Y. Role of ubiquilin associated with protein-disulfide isomerase in the endoplasmic reticulum in stress-induced apoptotic cell death. J. Biol. Chem. 2002, 277, 35386–35392.
- Wilkinson, B.; Gilbert, H.F. Protein disulfide isomerase. Biochim. Biophys. Acta 2004, 1699, 35–44.
- Schröder, M.; Kaufman, R.J. The mammalian unfolded protein response. Annu. Rev. Biochem. 2005, 74, 739–789, doi:10.1146/annurev.biochem.73.011303.074134.
- Hebert, D.N.; Bernasconi, R.; Molinari, M. ERAD substrates: Which way out? Semin. Cell Dev. Biol. 2010, 21, 526–532, doi:10.1016/j.semcdb.2009.12.007.
- Araki, E.; Oyadomari, S.; Mori, M. Impact of endoplasmic reticulum stress pathway on pancreatic β-cells and diabetes mellitus. Exp. Biol. Med. 2003, 228, 1213–1217.
- Ryu, E.J.; Harding, H.P.; Angelastro, J.M.; Vitolo, O.V.; Ron, D.; Greene, L.A. Endoplasmic reticulum stress and the unfolded protein response in cellular models of parkinson’s disease. J. Neurosci. 2002, 22, 10690–10698.
- Chan, S.L.; Culmsee, C.; Haughey, N.; Klapper, W.; Mattson, M.P. Presenilin-1 mutations sensitize neurons to DNA damage-induced death by a mechanism involving perturbed calcium homeostasis and activation of calpains and caspase-12. Neurobiol. Dis. 2002, 11, 2–19, doi:10.1006/nbdi.2002.0542.
- Klionsky, D.J.; Emr, S.D. Autophagy as a regulated pathway of cellular degradation. Science 2000, 290, 1717–1721, doi:10.1126/science.290.5497.1717.
- Levine, B.; Mizushima, N.; Virgin, H.W. Autophagy in immunity and inflammation. Nature 2011, 469, 323–335, doi:10.1038/nature09782.
- Høyer-Hansen, M.; Jäättelä, M. Connecting endoplasmic reticulum stress to autophagy by unfolded protein response and calcium. Cell Death Differ. 2007, 14, 1576–1582, doi:10.1038/sj.cdd.4402200.
- Ravikumar, B.; Sarkar, S.; Davies, J.E.; Futter, M.; Garcia-Arencibia, M.; Green-Thompson, Z.W.; Jimenez-Sanchez, M.; Korolchuk, V.I.; Lichtenberg, M.; Luo, S. Regulation of mammalian autophagy in physiology and pathophysiology. Physiol. Rev. 2010, 90, 1383–1435, doi:10.1152/physrev.00030.2009.
- Wang, K.; Klionsky, D.J. Mitochondria removal by autophagy. Autophagy 2011, 7, 297–300, doi:10.4161/auto.7.3.14502.
- Singh, R.; Kaushik, S.; Wang, Y.; Xiang, Y.; Novak, I.; Komatsu, M.; Tanaka, K.; Cuervo, A.M.; Czaja, M.J. Autophagy regulates lipid metabolism. Nature 2009, 458, 1131–1135, doi:10.1038/nature07976.
- Mathew, R.; Kongara, S.; Beaudoin, B.; Karp, C.M.; Bray, K.; Degenhardt, K.; Chen, G.; Jin, S.; White, E. Autophagy suppresses tumor progression by limiting chromosomal instability. Genes Dev. 2007, 21, 1367–1381, doi:10.1101/gad.1545107.
- Kim, J.; Kundu, M.; Viollet, B.; Guan, K.-L. Ampk and mtor regulate autophagy through direct phosphorylation of ulk1. Nat. Cell Biol. 2011, 13, 132–141, doi:10.1038/ncb2152.
- Liang, C.; Feng, P.; Ku, B.; Dotan, I.; Canaani, D.; Oh, B.-H.; Jung, J.U. Autophagic and tumour suppressor activity of a novel beclin1-binding protein uvrag. Nat. Cell Biol. 2006, 8, 688–698, doi:10.1038/ncb1426.
- Qu, X.; Yu, J.; Bhagat, G.; Furuya, N.; Hibshoosh, H.; Troxel, A.; Rosen, J.; Eskelinen, E.-L.; Mizushima, N.; Ohsumi, Y. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J. Clin. Invest. 2003, 112, 1809–1820.
- Yue, Z.; Jin, S.; Yang, C.; Levine, A.J.; Heintz, N. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc. Natl. Acad. Sci. USA 2003, 100, 15077–15082.
- Guertin, D.A.; Sabatini, D.M. Defining the role of mtor in cancer. Cancer Cell 2007, 12, 9–22, doi:10.1016/j.ccr.2007.05.008.
- Diaz-Troya, S.; Perez-Perez, M.E.; Florencio, F.J.; Crespo, J.L. The role of tor in autophagy regulation from yeast to plants and mammals. Autophagy 2008, 4, 851–865.
- Jung, C.H.; Ro, S.-H.; Cao, J.; Otto, N.M.; Kim, D.-H. Mtor regulation of autophagy. FEBS Lett. 2010, 584, 1287–1295, doi:10.1016/j.febslet.2010.01.017.
- Kerr, J.F.; Wyllie, A.H.; Currie, A.R. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 1972, 26, 239–257, doi:10.1038/bjc.1972.33.
- Hanahan, D.; Weinberg, R.A. The hallmarks of cancer. Cell 2000, 100, 57–70, doi:10.1016/S0092-8674(00)81683-9.
- Ghobrial, I.M.; Witzig, T.E.; Adjei, A.A. Targeting apoptosis pathways in cancer therapy. CA Cancer J. Clin. 2005, 55, 178–194, doi:10.3322/canjclin.55.3.178.
- Debnath, J.; Baehrecke, E.H.; Kroemer, G. Does autophagy contribute to cell death? Autophagy 2005, 1, 66–74, doi:10.4161/auto.1.2.1738.
- Ogata, M.; Hino, S.-I.; Saito, A.; Morikawa, K.; Kondo, S.; Kanemoto, S.; Murakami, T.; Taniguchi, M.; Tanii, I.; Yoshinaga, K. Autophagy is activated for cell survival after endoplasmic reticulum stress. Mol. Cell. Biol. 2006, 26, 9220–9231, doi:10.1128/MCB.01453-06.
- McLean, K.; VanDeVen, N.A.; Sorenson, D.R.; Daudi, S.; Liu, J.R. The HIV protease inhibitor saquinavir induces endoplasmic reticulum stress, autophagy, and apoptosis in ovarian cancer cells. Gynecol. Oncol. 2009, 112, 623–630, doi:10.1016/j.ygyno.2008.11.028.
- Bursch, W.; Ellinger, A.; Kienzl, H.; Török, L.; Pandey, S.; Sikorska, M.; Walker, R.; Hermann, R.S. Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells (MCF-7) in culture: The role of autophagy. Carcinogenesis 1996, 17, 1595–1607, doi:10.1093/carcin/17.8.1595.
- Rossignol, J.F.; Ayoub, A.; Ayers, M.S. Treatment of diarrhea caused by giardia intestinalis and Entamoeba histolytica or E. Dispar: A randomized, double-blind, placebo-controlled study of nitazoxanide. J. Infect. Dis. 2001, 184, 381–384, doi:10.1086/322038.
- Lam, K.K.; Zheng, X.; Forestieri, R.; Balgi, A.D.; Nodwell, M.; Vollett, S.; Anderson, H.J.; Andersen, R.J.; Av-Gay, Y.; Roberge, M. Nitazoxanide stimulates autophagy and inhibits mTORC1 signaling and intracellular proliferation of mycobacterium tuberculosis. PLoS Pathog. 2012, 8, e1002691, doi:10.1371/journal.ppat.1002691.
- Hong, S.K.; Kim, H.J.; Song, C.S.; Choi, I.S.; Lee, J.B.; Park, S.Y. Nitazoxanide suppresses IL-6 production in LPS-stimulated mouse macrophages and TG-injected mice. Int. Immunopharmacol. 2012, 13, 23–27, doi:10.1016/j.intimp.2012.03.002.
- Grivennikov, S.I.; Karin, M. Inflammatory cytokines in cancer: Tumour necrosis factor and interleukin 6 take the stage. Ann. Rheum. Dis. 2011, 70, i104–i108, doi:10.1136/ard.2010.140145.
- Naugler, W.E.; Karin, M. The wolf in sheep's clothing: The role of interleukin-6 in immunity, inflammation and cancer. Trends Mol. Med. 2008, 14, 109–119, doi:10.1016/j.molmed.2007.12.007.
- Lane, D.; Matte, I.; Rancourt, C.; Piché, A. Prognostic significance of IL-6 and IL-8 ascites levels in ovarian cancer patients. BMC Cancer 2011, doi:10.1186/1471-2407-11-210.
- Robinson-Smith, T.M.; Isaacsohn, I.; Mercer, C.A.; Zhou, M.; van Rooijen, N.; Husseinzadeh, N.; McFarland-Mancini, M.M.; Drew, A.F. Macrophages mediate inflammation-enhanced metastasis of ovarian tumors in mice. Cancer Res. 2007, 67, 5708–5716, doi:10.1158/0008-5472.CAN-06-4375.
- Davies, P.; Lineweaver, C. Cancer tumors as Metazoa 1.0: Tapping genes of ancient ancestors. Phys. Biol. 2011, doi:10.1088/1478-3975/8/1/015001.
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