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Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research
Brain Cancer Research Unit & Leukaemia Foundation Research Unit, Queensland Institute of Medical Research, Brisbane, QLD 4006, Australia
Queensland Brain Institute, University of Queensland, Brisbane, QLD 4067, Australia
Kenneth G. Jamieson Department of Neurosurgery, Royal Brisbane and Women's Hospital, Brisbane, QLD 4024, Australia
Cancer Services, Royal Brisbane and Women's Hospital, Brisbane, QLD 4024, Australia
Pathology Department, Royal Brisbane and Women's Hospital, Brisbane, QLD 4024, Australia
Oncogenic Signalling Laboratory, Centre for Cancer Research, Monash Institute of Medical Research, Melbourne, VIC 3168, Australia
Department of Medicine, University of Queensland, Brisbane, QLD 4006, Australia
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 February 2013; in revised form: 18 March 2013 / Accepted: 22 March 2013 / Published: 3 April 2013
Abstract: Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.
Keywords: glioblastoma; glioma; brain cancer; CUSA; surgical aspirate; multiplex flow cytometric analysis; brain cancer stem cells; tumor heterogeneity
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Day, B.W.; Stringer, B.W.; Wilson, J.; Jeffree, R.L.; Jamieson, P.R.; Ensbey, K.S.; Bruce, Z.C.; Inglis, P.; Allan, S.; Winter, C.; Tollesson, G.; Campbell, S.; Lucas, P.; Findlay, W.; Kadrian, D.; Johnson, D.; Robertson, T.; Johns, T.G.; Bartlett, P.F.; Osborne, G.W.; Boyd, A.W. Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research. Cancers 2013, 5, 357-371.
Day BW, Stringer BW, Wilson J, Jeffree RL, Jamieson PR, Ensbey KS, Bruce ZC, Inglis P, Allan S, Winter C, Tollesson G, Campbell S, Lucas P, Findlay W, Kadrian D, Johnson D, Robertson T, Johns TG, Bartlett PF, Osborne GW, Boyd AW. Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research. Cancers. 2013; 5(2):357-371.
Day, Bryan W.; Stringer, Brett W.; Wilson, John; Jeffree, Rosalind L.; Jamieson, Paul R.; Ensbey, Kathleen S.; Bruce, Zara C.; Inglis, Po; Allan, Suzanne; Winter, Craig; Tollesson, Gert; Campbell, Scott; Lucas, Peter; Findlay, Wendy; Kadrian, David; Johnson, David; Robertson, Thomas; Johns, Terrance G.; Bartlett, Perry F.; Osborne, Geoffrey W.; Boyd, Andrew W. 2013. "Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research." Cancers 5, no. 2: 357-371.