- freely available
Cancers 2012, 4(4), 1106-1145; doi:10.3390/cancers4041106
Published: 26 October 2012
Abstract: Prostate cancer is the third leading cause of male cancer deaths in the developed world. The current lack of highly specific detection methods and efficient therapeutic agents for advanced disease have been identified as problems requiring further research. The integrins play a vital role in the cross-talk between the cell and extracellular matrix, enhancing the growth, migration, invasion and metastasis of cancer cells. Progression and metastasis of prostate adenocarcinoma is strongly associated with changes in integrin expression, notably abnormal expression and activation of the β3 integrins in tumour cells, which promotes haematogenous spread and tumour growth in bone. As such, influencing integrin cell expression and function using targeted therapeutics represents a potential treatment for bone metastasis, the most common and debilitating complication of advanced prostate cancer. In this review, we highlight the multiple ways in which RGD-binding integrins contribute to prostate cancer progression and metastasis, and identify the rationale for development of multi-integrin antagonists targeting the RGD-binding subfamily as molecularly targeted agents for its treatment.
|Akt||protein kinase B|
|EGFR||epidermal growth factor receptor|
|EMT||epithelial mesenchymal transition|
|ERK||extracellular signal-regulated kinase|
|FACS||fluorescence activated cell sorting|
|FAK||focal adhesion kinase|
|GRPR||gastrin releasing peptide receptor|
|Hsp90||heat shock protein 90|
|HUVEC||human umbilical vein endothelial cell|
|IGF-1R||insulin-like growth factor type 1 receptor|
|LAP||latency associated peptide|
|MEK||mitogen-activated protein kinase/extracellular signal-regulated kinase kinase|
|MRI||magnetic resonance imaging|
|PET||positron emission tomography|
|PSA||prostate specific antigen|
|SIBLING||small integrin binding N-linked glycoprotein|
|SMART||Simultaneously Multiple Aptamers and RGD Targeting|
|SPARC||secreted protein acidic and rich in cysteine|
|TCIPA||tumour cell-induced platelet aggregation|
|TGF-β||transforming growth factor-β|
|TNF-α||tumour necrosis factor-α|
|VEGF(R)||vascular endothelial growth factor (receptor)|
|vWF||von Willebrand factor|
Standard 1 letter codes for amino acids are used throughout.
Prostate cancer is the most commonly occurring cancer in men in the developed world, and the second most common worldwide, with over 900,000 new cases and 250,000 deaths estimated to occur annually . Survival rates are increasing, largely due to improvements in detection and treatment of early stage disease, however, the treatment of advanced prostate cancer remains a challenge. As the disease progresses, tumours become castration resistant and no longer respond to hormonal deprivation therapies . Bone metastases are a common occurrence in castration-resistant prostate cancer, affecting up to 80% of patients, and result in significantly reduced quality of life through pain and pathological fractures . Metastases to other sites, such as distal lymph nodes and lung, are also features of advanced disease. There are currently few effective therapeutic agents for advanced prostate cancer, with the most frequently used agent, docetaxel, often poorly tolerated . Although a number of new drugs such as abiretarone acetate and alpharadin are progressing into the clinic , there remains a need to identify molecularly targeted agents to control tumour dissemination for survival benefit. Furthermore, discovery of biomarkers of disease progression will provide new diagnostics for early detection and prognostic indicators for treatment outcome.
The integrin family of cell surface glycoproteins control cell-extracellular matrix (ECM) adhesion and signalling across the cell membrane. Integrins are heterodimers made up of an α and a β glycoprotein subunit, each comprising a multidomain extracellular section which interacts with extracellular proteins, a single transmembrane domain, and a short cytoplasmic tail which interacts with intracellular signalling effectors and kinases [5,6].
Integrins can change conformation in response to intracellular signals or extracellular ligand binding; the active conformation is characterised by extension of the receptor headpiece above the cell surface, increased affinity for ligands, and increased integrin-mediated signalling [7,8,9]. A useful pictorial summary of integrins and their ligands has been published by Humphries .
Integrins may be divided into classes according to cellular expression, subunit identity or ligands bound [11,12]. The RGD-recognising integrins are a family of 8 integrins (Table 1) which bind the common Arg-Gly-Asp tripeptide sequence at a binding site formed at the junction of the α and β subunit headpieces. α5β1 and α8β1 possess synergy sites in addition to the RGD binding site, where the binding of PHSRN and LFEIFEIER sequences increases ligand integrin affinity. Considerable detail regarding ligand binding is available for integrins that have become the focus for drug design: crystal structures have been determined for αIIbβ3 , αvβ3 [14,15,16] and α5β1 , and homology models for αvβ5  and α5β1 .
|Integrin||Common Ligands||Major physiological roles|
|αvβ1||Fibrinogen (Fg), Fibronectin (Fn), Vitronectin (Vn), Osteopontin (OPN), LAP-TGF-β|
|αvβ3||Fg, Von Willebrand factor (vWF), Fn, Vn, Bone sialoprotein (BSP), OPN, LAP-TGF-β||Angiogenesis Bone resorption|
|αvβ5||Fn, Vn, BSP, LAP-TGF-β||Angiogenesis Vascular permeability|
|αvβ6||Fn, Vn, OPN, LAP-TGF-β||Lung development and physiology TGF-β activation|
|αvβ8||Vn, LAP-TGF-β||TGF-β activation Angiogenesis Brain development|
|α8β1||Fn, Vn, Tenascin, OPN, LAP-TGF-β, Nephronectin||Kidney and lung development Hair cell differentiation and function|
|αIIbβ3||Fg, Fn, Vn, vWF||Platelet aggregation|
A number of the RGD-recognising integrins, αvβ3, αvβ5, αvβ8 and α5β1, play important roles in controlling angiogenesis [27,28]. αvβ3 is directly involved in bone turnover through mediating osteoclast adhesion and function  and thus is identified as a drug target for treatment of osteoporosis and bone metastasis [30,31]. αvβ3 expression on other cell types, and other integrins, including αvβ5 and α5β1 expressed on cancer and endothelial cells, and platelet αIIbβ3  also contribute to the process of bone metastasis, facilitating tumour cell survival in the blood stream, migration, and adhesion at the metastatic site [31,32,33,34].
Previous reviews have covered detailed aspects of integrin expression and signalling in cancer , including the non-RGD binding integrins such as α2β1, α6β1 and α6β4 in prostate cancer [32,36,37,38]. Here, we summarise key findings on the expression and function of the RGD-binding integrin subfamily in prostate cancer, and review drugs and imaging agents currently in development that target these receptors.
2. Expression of RGD-Binding Integrins in Prostate Cancer
2.1. Clinical Prostate Adenocarcinoma
The expression of RGD-binding integrins in clinical tissue samples is summarised in Table 2. Primary cells isolated from tissue samples of prostate adenocarcinoma expressed high levels of functional αvβ3, in contrast to epithelial cells from normal tissue which are αvβ3 negative. FACS analysis for αv, α5 and β1 integrin subunits showed little difference in expression between cells from tumour and normal tissue, suggesting αvβ3 is responsible for tumour cell migration towards and adhesion to vitronectin . The expression of the αv subunit in primary cultures has subsequently been shown to be highly variable (0.1–51.9% of cells), and indicative of the tumour stem cell population in a particular sample .
|Table 2. Summary of RGD-binding integrin expression observed in prostate adenocarcinoma.|
|Integrin||Expressed in normal prostate tissue?||Expressed in primary prostate tumours?||Expressed in metastases?||Reference|
Prostate cancer is one of a small number of tumours that ectopically express αIIbβ3, an integrin originally believed to be found only on platelets. αIIb mRNA was originally detected in human tumour samples by in situ hybridisation ; αIIb protein expression has since been confirmed by other techniques .
Truncated forms of integrin subunits have also been found in tumour samples. Truncated variants of both αIIb  and β3  have been detected in tumour samples with intermediate or advanced Gleason grade. Both truncated forms are also expressed in DU145 and PC-3 prostate cancer cell lines, and have been shown to be secreted by the cells and prevent their adhesion to integrin ligands. It is interesting to speculate that expression of truncated integrins could facilitate tumour migration by diminishing ECM adherence.
An analysis of the association between tumour integrin expression and the likelihood of biochemical recurrence after surgical removal of an apparently localised tumour found the majority of 111 prostate tumours expressed αv, αvβ3 and αIIbβ3 integrins . The pattern of αv and αvβ3 expression was the same in recurrent and non-recurrent tumours; 25–28% of each group showed no expression, while the majority were classified as moderate or high expressing. Over 90% of tumours were αIIbβ3-positive. αIIbβ3 expression was stronger in recurrent tumours (40% strongly expressing compared to 20% of non-recurrent tumours), and was identified as marginally significant for recurrence, whereas high expression of α3β1 was highly significant as a prognostic indicator.
In contrast, a comparison of paired samples of primary prostate tumours and lymph node metastasis from 19 patients found “abnormal” expression of αv and αvβ3 in all cases. Expression was classified as abnormal if immunohistochemical staining was negative, weak, moderate or focal. Metastasis was frequently associated with a decrease in integrin expression, with αv expression increasing in 6% of cases and decreasing in 59%, and αvβ3 expression decreasing in 47% of cases . These results should be interpreted with caution since strong expression of αvβ3 only occurs normally on activated endothelial cells. Weak or moderate ectopic expression of a functional integrin could be highly significant for cell proliferation and spreading. An observational cohort study on 64,545 men provided 1,172 cases of prostate cancer where samples could be analysed to determine molecular markers of aggressive disease. Unfortunately, β3 integrin expression could not be detected by immunohistochemical analysis in the archival tumour samples .
Normal prostate tissue has been reported to express αvβ1 but not α5β1 . In 20 cases of primary prostate cancer, one expressed α5β1; the expression of αv and other β subunits was not reported . Expression of the α5 and β1 subunits has been shown to be negatively correlated with clinical tumour grade, with a comparison of 30 primary prostate tumours and 30 normal prostate samples showing a significant reduction of α5 and β1 expression in the tumour samples . In contrast, a comparison of biopsy samples from benign prostatic hypertrophy and primary prostate tumours found β1 expression increased with tumour grade and became located on the surface of tumour cells. Low levels of β1 expression were observed in areas of benign disease, although these samples included apparently normal biopsies from patients with diagnosed prostate cancer . Weak β3 expression was also present in 25% of tumour areas.
A meta-analysis of genes involved in prostate cancer progression noted a general trend for downregulation of integrins (both RGD and non-RGD binding) and their ligands (notably, changing the expression pattern of collagens) during cancer progression . ITGAV and ITGB5 were upregulated in prostatic intraepithelial neoplasia compared to normal prostate, and in the transition from normal prostate to non-metastatic cancer ITGA5, B1, B3 and B6 were downregulated, and ITGA2B, AV and B5 were upregulated. Expression of ITGA5 was negatively correlated with Gleason score. It has been proposed that the decrease in integrin expression allows cells to escape integrin-mediated cell death resulting from decreased collagen expression as part of the normal aging process, and leads to increased cell motility and proliferation driving tumourigenesis. However, further work is required to correlate this analysis of integrin message with integrin protein expression.
Screening a tissue microarray of archival human tumours using a panel of antibodies to αv subfamily integrins showed prostate cancer samples strongly expressed αv and αvβ5 in the tumour cells and stroma, with some expression of αvβ3 on the tumour vasculature. Small regions of tumour were positive for αvβ6, and there was no expression of αvβ8 .
Studies on the expression of αvβ6 show similar contradictory results to those on αvβ3. Immunohistochemical analysis of 40 primary prostate tumours found 77% were αvβ6 negative, but high integrin expression was observed in basal cells in ducts with high-grade intraepithelial neoplasia and in normal tissue adjacent to tumours . Other studies have shown that αvβ6 is expressed in epithelial cells in prostate tumours, and focally in areas of inflammation, proliferative inflammatory atrophy, and prostate intraepithelial neoplasia . Similar expression patterns were observed in transgenic mouse models of prostate disease. In the Ptenpc−/− model of spontaneous prostate cancer, αvβ6 was expressed on malignant epithelial cells in prostatic intraepithelial neoplasia and invasive adenocarcinoma. αvβ6 expression was also induced in the POET model of prostate inflammation. αvβ6 is proposed to support metastasis by activating TGF-β1, which is associated with metastasis and poor prognosis .
Characteristic patterns of integrin expression have been found present in bone metastasis. Tumour cells obtained from the bone marrow of patients without overt metastases expressed α5, αv, β1 and β3 integrin subunits, a pattern which appeared to be characteristic of micrometastatic cells, and which was hypothesised to promote homing and survival in bone marrow . These integrins may therefore represent potential targets in preventing bone colonisation. αvβ3 and αvβ5 have been found to be expressed in the active conformation on prostate tumours; comparison of matched samples of primary tumour and bone metastasis showed that integrin activation is increased in bone metastasis compared to the primary tumour . Increased expression of the β3 subunit has been found in prostate cancer orbital metastases . β3 expression is hypothesised to be responsible for the tendency of prostate cancer to metastasise to bone, but despite substantial evidence for this role in preclinical models, and demonstrated efficacy of integrin antagonists in treating bone metastasis (vide infra) there have been no large studies on integrin expression in bone metastases.
2.2. Preclinical Models of Prostate Cancer
Compared to other types of cancer, a relatively small number of cell line models are commonly used to study prostate adenocarcinoma.Reports of the expression of RGD-binding integrins in these commonly used prostate carcinoma cell line models are summarised in Table 3 and Table 4.
|Table 3. RGD-binding integrin heterodimer expression on the surface of prostate cancer cell lines. Key: +++ very high; ++ high; + moderate; +/− low; − negative; blank not tested; ns expressed but level not stated.|
|Table 4. RGD-binding integrin subunit expression on the surface of prostate cancer cell lines. Key: +++ very high; ++ high; + moderate; +/− low; − negative; blank not tested; ns expressed but level not stated.|
It is noteworthy that contrasting patterns of expression are frequently observed between cell lines used in different laboratories; for example, the expression of αvβ3 in both PC-3 and LNCaP cells ranges from high to absent, an observation that has been attributed to response to differences in cell culture conditions, or the development of clonal variations or cell line heterogeneity in long term culture [63,65]. The variability of expression of αvβ3 and αvβ6 in cell lines parallels that seen in tumour samples whereas αvβ5 and α5β1 are generally always expressed in cell lines. αvβ6, and αvβ8 expression is less well-investigated compared to other integrins, and there are no reported studies on α8β1.
To improve understanding and reproducibility of models of prostate cancer, changes in integrin expression in response to the environment in which a cell line is grown have been investigated. In cells grown in culture, the expression of all integrins is significantly downregulated on the surface of highly confluent cells . αIIbβ3 surface expression was observed to be higher in orthotopically implanted DU145 tumours than in subcutaneous xenografts; ectopic expression of αIIbβ3 decreased when the cells were cultured . αv gene expression was upregulated in PC-3 cells grown in contact with normal prostate stromal cells, and downregulated in contact with long bone osteoblasts . Expression of αv and β3 mRNA measured by quantitative PCR was correlated with tumourigenicity in LNCaP, C4-2 and PC-3 cell lines grown in culture and as xenografts, although αvβ3 protein levels were similar in all cell lines and lowest in PC-3 . Changes in integrin mRNA levels when cell lines were grown as xenografts were cell line dependent; expression increased in the more tumourigenic cell lines, with a very large increase in β3 levels observed in PC-3 xenografts . Similarly, exposure of LNCaP to dehydrotestosterone increased the expression of αv and VEGF mRNA, but had no effect on PC-3 . In vivo, factors in the local microenvironment, which are not normally present in cell culture media, can control integrin expression. For example, intratesticular inoculation increased αv mRNA expression in both LNCaP and PC-3 xenografts, and resulted in increased tumour growth and metastasis compared to orthotopic implantation of LNCaP, due to enhanced angiogenesis and interaction with the tumour stroma .
Integrin receptor expression can change over time, due to tumour progression and intratumour heterogeneity in in vivo models and clinical cases, and due to development of clonal variations and adaptation to the levels of protein ligands available when cell lines are grown in culture. Additionally, integrin expression in xenografts can change in response to the tumour environment and thus differ markedly from the same tumour model in vitro. It is therefore important that tumour models used to investigate integrin biology or integrin-targeted drugs be fully characterised to ensure they retain the receptors of interest in a particular situation, for example when cultured in specific media/cell density or grown as xenografts. Most current studies do not assess the full integrin profile of the cell lines used; this is an important consideration when assessing the biological response to antagonists with different receptor selectivity profiles, given the ability of multiple integrins to recognise the same ligands, control the same physiological processes, and interact with one another through trans-dominant inhibition.
3. Consequences of Integrin Expression in Prostate Cancer
3.1. αv Subfamily Integrins
The most thoroughly investigated αv subfamily heterodimer, αvβ3, is overexpressed on activated endothelial cells and in a wide range of cancer types . Its role in prostate cancer was previously reviewed by Cooper et al. in 2002 . αvβ3 expression and abnormal activation in cancer is commonly associated with disease progression and poor prognosis . As cell lines develop a more metastatic phenotype, integrin use changes and αvβ3 expression increases; C4-2 requires αvβ3 for adhesion and migration, whereas the parent LNCaP cell line used αvβ5 for adhesion and was unable to migrate on OPN . Transformation of LNCaP to an androgen-independent model of prostate cancer, LNCaP-19 resulted in increased cell proliferation, migration, in vivo invasive ability, and adhesion to vitronectin and fibronectin. However the expression of αv, β3, α5 and β1 was unchanged, suggesting that increased integrin activation may be responsible for the observed increase in cells’ adhesive properties .
αvβ3 expression allows cells to adhere to and migrate on Vn [39,67,78], Fn [39,67] and OPN . Binding to Vn activates signalling through FAK  and PI3K/Akt pathways; activation of PI3K/Akt signalling is specifically required for adhesion and migration on Vn to take place  and Akt1 activity has been shown to promote adhesion, migration on ECM ligands and transendothelial migration by regulating β3 integrin activation .
αvβ3 and α5β1 mediate prostate cancer cell interactions with endothelial cells through adhesion to Vn and Fn on the endothelial cell surface and in their surroundings . β3 integrin in endothelial cells are associated with focal contacts—regions of firm adhesion—whereas in PC-3 cells, β3 is located in pseudopodia and membrane ruffles associated with cell motility. Use of antisense oligonucleotides for β3 has shown that functional β3 is required for the later stages of transendothelial migration; it enables cells to extend membrane protrusions and interact with the underlying ECM proteins .
Binding to Vn has been recently demonstrated to induce differentiation of prostate cancer stem cells; αvβ3 is the most important receptor controlling differentiation, with αvβ5 making a smaller contribution. Antagonism of αvβ3 with anti-αvβ3 antibodies or the cyclic peptide cRGDfK prevented stem cell differentiation, activation of FAK signalling, and development of tumours in vivo following injection of stem cells . The discovery that αvβ3-engagement and signalling is necessary for tumourigenesis is an important one: maintaining stem cells in a dormant state by use of integrin antagonists represents a new strategy for targeted therapy.
Crude extract of proteins from mineralised bone supported adhesion of DU145 cells which was blocked by RGD peptide or anti-αvβ3 antibody . RGD-containing integrin ligands found at high levels in the bone microenvironment include Fn, Vn, OPN, and other members of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins, namely BSP, dentin matrix protein 1, dentin sialophosphoprotein (DSPP), and matrix extracellular phosphoglycoprotein . Expression of SIBLINGS, notably BSP and OPN is associated with prostate cancer progression [84,85,86,87]; OPN  and BSP  can be used as biomarkers of bone metastasis and DSPP has been proposed as a biomarker for prostate cancer diagnosis .
Osteopontin is a chemoattractant for prostate cancer cells, potentially promoting preferential formation of bone tumours . OPN increases the metastatic capability of a cell by increasing the secretion of plasminogen activators  and MMP-9  and the formation of invadopodia (cell membrane structures used in adhesion and migration) , all through αvβ3 signalling pathways. αv-integrin mediated binding of prostate epithelial cells to OPN stimulated the growth of a subpopulation of cells with high proliferative potential, possibly stem cells . Subsequently, the av subunit has been shown to be a marker of prostate cancer stem cells . Bone sialoprotein has also been shown to increase expression of MMPs, promote integrin-mediated migration, and upregulate integrin survival signalling pathways in prostate carcinoma cells. Transfection of PC-3 cells with BSP increased expression of αv, β3 and β5, formation of focal adhesions, and integrin-mediated migration and survival signalling; blocking integrin-BSP interaction and the resulting signalling through αvβ3/αvβ5 could provide a new anti-metastatic strategy .
A number of proteins have been implicated as driving factors for bone-specific metastasis. αvβ3 and αvβ5 mediate the preferential migration of metastatic prostate cancer cells to bone-derived secreted protein acidic and rich in cysteine (SPARC). Tumour growth and migration are supported by an autocrine loop, where SPARC binding to αvβ5 results in increased levels of VEGF and VEGFR2, which in turn activate αvβ3 and αvβ5 and stimulate cell proliferation. Upregulation of VEGF production by SPARC provides metastatic prostate cancer cells with a specific growth advantage in the bone microenvironment, thus blocking the interaction of SPARC with activated αv integrins should reduce bone metastasis . Studies with isogenic prostate cancer cell lines expressing αvβ3 in a range of activation states have further shown that functionally active αvβ3 is required for the growth of bone metastasis. αvβ3-negative tumour cells promote bone loss, but αvβ3 is required for bone deposition and an osteoblastic phenotype. Locking αvβ3 in an inactive or active conformation reduced tumour growth; inactive αvβ3 is unable to support cell adhesion, whereas permanently active integrin supports strong adhesion but cannot regulate adhesion or signalling .
Exposure of bone marrow mesenchymal stems cells to conditioned medium from bone metastatic prostate cancer cells upregulated expression of α5 and αv mRNA more than 8-fold; β1 and β3 expression was increased by a lesser extent. Expression of the integrin ligand Fn was also significantly increased. These changes promote differentiation into osteoblasts, initiating a mechanism for formation of new bone once metastasis has become established in the bone . Exposure of metastatic prostate cancer cells to stromal cell-derived factor 1 increased the expression of high affinity (activated) αvβ3, promoting adhesion to bone marrow endothelial cells, VN, and OPN. Stromal cell-derived factor 1 is present at high levels in the bone microenvironment, therefore may promote integrin-mediated adhesion, invasion, and proliferation of metastatic cells once they reach the bone marrow . Bone morphogenetic protein-2 secreted by osteoblasts induced migration in both androgen dependent and androgen independent prostate cancer cell lines by upregulating β1 and β3 integrins on the cell surface through an Akt, ERK, IKKα/β, NF-κB signalling pathway. The effects of bone morphogenetic protein-2 were blocked by kinase inhibitors or integrin knockdown , suggesting it to be a potential target in blocking the spread of bone metastasis.
αvβ3 on osteoclasts also plays a significant role in the development of bone metastasis. Functional osteoclasts are required for both physiological and pathological bone remodelling, with pathological destruction of bone being necessary for a metastasis to gain space to develop. Knockdown of β3 in bone marrow, or other disruption of osteoclast function, protects mice from the development of osteolytic bone metastases, indicating that interaction between tumour cells and osteoclasts are required for tumour growth and bone destruction .
In a PC-3 model lacking αvβ3, adhesion and migration on Vn was mediated by αvβ1 and αvβ5 . siRNA knockdown of αv caused regression of bone xenograft tumours, but had no effect on subcutaneous xenografts, indicating that integrin-mediated interactions with bone stroma are specifically required for the survival of bone metastasis. The ability of multiple RGD-binding integrins to support such interactions suggests that multi-integrin targeted drugs will be required for integrin-targeted anti-metastatic therapy to be effective. Interaction with the bone stroma has also been shown to promote resistance to radiotherapy, and treatment with the anti-αv antibody CNTO-95 reversed this effect in vitro . The mechanism of αvβ3 induced radioresistance has been shown to involve maintaining high levels of survivin .
A large number of signalling pathways affect integrin expression thus inducing downstream effects in prostate cancer cells. For example, leptin signalling upregulates αvβ3 expression and thus promotes cell migration. Increased cell migration in response to leptin can be blocked by inhibition of the kinase signalling pathway or by blocking integrin function . High levels of calcitonin and its receptor correlate with tumour grade. Calcitonin activates the urokinase receptor (uPAR) and promotes migration on Vn through increased αvβ3 levels and promotion of αvβ3-uPAR association and FAK signalling . Exposure of prostate cancer cells to prostaglandin E2 induces phosphorylation of EGFR, MEK, ERK1/2, and promotes expression and phosphorylation of β3 integrin . Subsequent signalling promotes cancer progression through activator protein-1 transcriptional control, and cell migration and angiogenesis through increasing uPA and VEGF secretion. Chemokine signalling has been implicated in controlling prostate cancer cell invasion, αvβ3 integrin clustering and localisation and thus cell adhesion .
αvβ6 has not been extensively investigated in prostate cancer, but is implicated in progression of a number of other cancers including gynaecological, head and neck and colon adenocarcinoma, where it has been shown to promote invasion and metastasis through activation of MMPs (in a similar manner to αvβ3) and activation of TGF-β1 . αvβ6 expression is associated with inflammation, and the β6 subunit is increased in cases of benign prostatic hypertrophy  as well as in prostate cancer, where αvβ6 has been implicated in controlling prostate cancer growth in conjunction with the androgen receptor . Increased transcriptional signalling from Stat3 in prostate cancer leads to increased expression of Fn and its receptor αvβ6, thus increased cell migration, anchorage independent growth and epithelial mesenchymal transition (EMT), which were inhibited by an anti-αvβ6 antibody . Constitutive Stat3 activation is associated with tumour progression; integrin antagonism is a potential therapeutic target to reverse its effects. αvβ6 has been shown to cooperate with αvβ1 and α5β1 to allow cells to migrate on Fn. This allowed cells to maintain the ability to migrate when treated with an antagonist selective for 1 or 2 integrins .
Little is known regarding the role of αvβ1 in cancer. αvβ1 expression has been shown to be controlled by the amount of the αv subunit available in the cell, and it is the first integrin to be downregulated as αv levels decrease , therefore it may gain a more prominent role if other αv-containing heterodimers are suppressed.
αvβ8 shares with αvβ6 the ability to activate latent TGF-β. αvβ8 has been shown to be a negative regulator of tumour growth in mouse models [106,107]. There is little data available on αvβ8 expression in human tumours, and the relevance of the model studies to human disease remains to be determined.
A systems biology approach to prostate cancer has identified key proteins which act as links between multiple signalling networks essential for tumour progression. Hsp90, Runx2 and αv integrin signalling have been identified as key to 3 networks, which are extensively interconnected to form a single supernetwork which will disintegrate when key molecules are targeted. αv integrin signalling controls tumour growth at all stages of disease, and both αv and Runx2 promote metastasis and tumour growth in the bone microenvironment . This study provides strong support for a causal effect of αv integrins in prostate cancer. The relative importance of individual members of the αv subfamily in the signalling network remains to be identified, however, the use of multi-integrin antagonists targeting the whole subfamily should efficiently disrupt the prostate cancer signalling network thus prevent tumour growth and progression.
The presence of αIIbβ3 on the cell surface of DU145 cells is associated with increased tumourigenicity, local invasion, and development of lymph node metastases. In contrast, PC-3 cells with intracellularly-located αIIbβ3 were less invasive. Antibodies to αIIbβ3 prevented lung colonisation by DU145 cells in the mouse tail vein injection model of the early steps of metastasis .
αIIbβ3 expression on platelets can mediate tumour-platelet interactions in the absence of tumoural αIIbβ3; tumour-platelet interactions have recently been reviewed by Buergy et al. . Contact with tumour cells stimulated platelet activation and aggregation, forming tumour-platelet aggregates, which promote tumour cell arrest in the circulatory system, an intermediate stage of metastasis. Platelet aggregation induced by PC-3 cells has been prevented by the αIIbβ3 antagonist peptide GRGDS and the disintegrins trigramin and rhodostomin . Platelet activation provides a source of soluble growth factors for promoting tumour growth at the metastatic site, and promotes the release of platelet-derived microparticles. Exposure to platelet-derived microparticles caused cancer cells to become αIIbβ3 expressing, increased angiogenesis and metastasis  and promoted invasion and MMP-2 production in prostate cancer cells .
αIIbβ3 antagonism has the potential to prevent metastatic spread by multiple mechanisms; preventing tumour platelet interaction through action of tumour or platelet integrin receptors thereby reducing tumour cell survival in the bloodstream, or by preventing cell adhesion, invasion or growth at the metastatic site. The small molecule αIIbβ3 antagonist ML464 has been shown to be effective at preventing bone metastasis and reducing visceral metastasis in a mouse model of melanoma, but has not yet been tested against prostate cancer .
α5β1 has been shown to control the proliferation of prostate cancer cells in 3D culture through formation of a signalling complex with insulin-like growth factor type 1 receptor (IGF-1R) . β1 integrin mediated binding to fibronectin protects DU145 cells against the cytotoxic effects of docetaxel, again through promoting IGF-1R-β1 complex formation . However, fibronectin did not protect cells against radiotherapy, possibly because exposure to high dose radiation reduces β1 expression and associated cell adhesion .
α5β1 controls fibronectin matrix assembly which in turn controls the organisation and stability of the ECM. The ability of prostate cancer cells to assemble a fibronectin matrix is correlated with the ability to form cohesive aggregates and cellular α5β1 expression. High α5β1 levels and fibronectin matrix assembly are inversely correlated with invasiveness; loss of α5β1 allows cell detachment, leading to intravasation and metastasis . Agonism of α5β1 has therefore been proposed as an anti-metastatic strategy and the MEK inhibitor AZD6244 has been shown to promote cell adhesion, possibly by activating α5β1 , however such an approach will require careful assessment for safety, since α5β1-mediated adhesion and angiogenesis could promote the establishment of metastases once cells have detached from the main tumour. Recent work has shown that the actin regulatory protein Mena is required for α5β1-mediated functions and signalling in fibroblasts . Upregulation of Mena, and expression of a pro-invasive isoform of the protein have been associated with metastasis in a number of cancers , suggesting that further studies of Mena may prove significant in the diagnosis and treatment of metastasis in future.
Tumour cell α5β1 and αvβ3 mediate adhesion of prostate cancer cells to endothelial cells through binding cell surface fibronectin and vitronectin . α5β1 and αvβ3 have contrasting roles in the early stages of adhesion; α5β1 mediated cell adhesion to and spreading on fibronectin, and whereas inhibition of αvβ3 had no effect on adhesion to and spreading on fibronectin, but promoted cell shape changes and reorganisation of cytoskeletal proteins, suggesting that αvβ3 is a negative regulator of other fibronectin-binding integrins . RGD peptides such as cRGDfV  and GRGDSP  were more effective at preventing cell attachment and spreading than specific anti-integrin antibodies, again demonstrating the limitation of selective integrin antagonists versus pan-integrin antagonists as anticancer agents. RGD peptides were also effective at preventing adhesion of prostate cancer cells to bone marrow endothelial cells, which express high levels of α5β1 .
Expression of α5β1 on prostate cancer cells is affected by a number of signalling pathways linked to tumour invasion and bone metastasis: the increase in cell adhesion to the endothelium caused by CXCL12 binding CXCR4 is a result of increased expression of α5 and β3 . Parathyroid hormone related protein increased cell adhesion and promoted cell survival by upregulating cell surface levels of α5, along with α1, α6 and β4 subunits [120,121]. EGF also increased cell adhesion to fibronectin and increased migration by increasing cell surface levels of the β1 subunit without a corresponding increase in α5 .
Re-expression of the androgen receptor in androgen resistant DU145 cells increased α5 levels on the cell surface and cells’ invasive ability, but reduced cell adhesion to a number of ECM substrates including fibronectin . Expression of Snail, a transcription factor associated with the epithelial mesenchymal transition, resulted in reduced cell adhesion and increased migration with decreased cell surface expression of α5 and β1 . Snail has also been shown to increase αvβ3 expression, and promote adhesion and migration to αvβ3 ligands such as OPN, suggesting it controls in site-specific relocation of cancer cells .
Alternative splicing of the β1 integrin subunit gives rise to variants with different properties in cancer cell lines; the normal β1 (β1A) subunit is found in integrins which promote prostate cancer cell proliferation, whereas the β1C variant inhibits cell proliferation. The expression of both β1 integrin variants is decreased at the mRNA level in prostate carcinoma, but only β1C protein expression is lost due to reduced transcription and translation, and increased degradation of β1C, combined with increased translation of β1A [126,127]. Loss of β1 integrin expression in androgen sensitive prostate cancer is reversible with short term neoadjuvant androgen deprivation therapy: expression of β1C mRNA was significantly increased in patients treated with androgen deprivation therapy due to increased transcription . The extent of reversal was negatively correlated with the tumours’ Gleason grade. No effect was observed with longer-term treatment , suggesting that β1C-mediated pathways are linked to androgen-mediated mechanisms and the development of resistance.
αv integrins play an important role in developmental angiogenesis, with αv knockdown being lethal in mice. This is possibly due to the effect of removing αvβ8 integrin expression on brain development , since mice lacking β3 or β5 develop normally and subsequently show enhanced pathological angiogenesis .
Elevated expression of αvβ3 and αvβ5 on active endothelial cells, and the efficacy of their antagonists in preventing angiogenesis in preclinical studies [131,132] has led to these integrins becoming popular targets for anti-angiogenic drug development. However, subsequent work has revealed the complexity of integrin function in this area, with multiple integrins on multiple cell types interacting to both promote and prevent angiogenesis [27,133].
α5β1 has a similar role to αv integrins during angiogenesis, with global α5 knockout being lethal; in endothelial cells, knockout of α5 alone had little effect on developmental angiogenesis due to compensation by αv, but knockout of both α5 and αv integrins was lethal due to defects in vascular remodelling . The use of non-selective integrin antagonists such as echistatin, or combination of selective α5β1, αvβ3 and αvβ5 inhibitory antibodies completely blocked tube formation by endothelial cells in a fibrinous exudate mimicking tumour stroma, whereas a selective α5β1 or αvβ3 antibody had little effect . In contrast, selective α5β1 antagonism has been shown to prevent lymphangiogenesis whilst allowing αv integrin mediated angiogenesis . These results suggest that multi-integrin antagonism will be required for efficient anti-angiogenic therapy in tumours, and suggest a possible explanation for the limited efficacy so far observed in clinical trials of αvβ3/αvβ5-targeted antiangiogenic agents.
3.5. Epithelial-Mesenchymal Transition
TGF-β upregulates expression of the RGD-binding integrins  and there is extensive crosstalk between TGF-β and αvβ3 , αvβ5 [139,140], αvβ6 [103,141], and α5β1 [142,143]. TGF-β also regulates EMT, where cells acquire the invasive, migratory phenotype required for metastasis. EMT is associated with increased αv expression [139,144]. In cancer, αvβ3 , αvβ5  and α5β1  have been shown to be important in allowing EMT to take place, and αvβ6 is induced during the process . Inhibition of RGD-binding integrins has been shown to cause invasive prostate cancer cells to revert to a non invasive epithelial phenotype , and to block EMT in other cancers .
The importance of TGF-β signalling  and EMT  in bone metastasis and the role of the upregulated integrins in promoting tumour invasion and metastasis  suggests the associated signalling pathways as a therapeutic target in advanced prostate cancer. However, most research linking EMT and integrin function is in non-prostate cancers, and specific roles in prostate cancer, and particularly in the mesenchymal to epithelial reverse transformation observed in established metastasis , remain to be established.
3.6. Laminin Binding Integrins
Changes in expression of other integrins containing the β1 subunit is also characteristic of prostate cancer progression. Expression of α2β1  and the laminin binding integrin α3β1  have been associated with disease progression and metastasis. Increased α6 surface expression [151,152] and a switch in the heterodimeric partner of α6 from α6β4 to α6β1  promotes cell migration and invasion. The α6 subunit is also cleaved by urokinase plasminogen activator, [153,154,155] to give the cancer-specific shortened subunit α6p which promotes metastasis. Prevention of α6 cleavage reduces prostate cancer cell migration and invasion, and reduces the development and effects of bone metastasis [156,157].
β1-containing integrins can interact with other RGD-binding integrins via trans-dominant inhibition, where expression of one integrin suppresses function of another by competing for intracellular activator proteins, or changes the expression of other integrins by altering mRNA stability and translation . Some examples of integrins interacting via transdominant inhibition include function modulating crosstalk between αvβ5 and α5β1 , αvβ3 or αvβ5/αvβ6 and α2β1 [160,161], and αvβ3 and α3β1 or α6β1 . Introducing β1 expression in a model system has been shown to decrease β3 and increase β5 protein levels . The αvβ3 antagonist cilengitide has been shown to reduce β1-mediated endothelial cell adhesion and survival, since it is able to activate αvβ3 as well as block the αvβ3 adhesion site . The significance of such interactions has not yet been explored in prostate cancer, and there is a need for more basic research in the general area of trans-dominant inhibition. From a translational perspective, the current data further emphasises the importance of fully characterising integrin expression in model systems so the presence of trans-dominant inhibition can be included in interpretations of results.
4. Changes in Integrin Expression in Response to Therapy
The interconnected nature of cell signalling pathways means that drugs targeting other receptors and kinases can affect the expression and function of integrins through modulating their transcription, transport or downstream signalling. Studies in this area are summarised in Table 5.
|Table 5. Alterations in cell surface expression of integrin subunits in response to treatment with non-integrin targeting drugs.|
|Drug||Cell line||Integrin subunits upregulated||Integrin subunits downregulated||Reference|
|Doxazosin||PC-3||β8 (mRNA)||α5, αv, β1, β5, β6 (mRNA)|||
|Camptothecin||PC-3||αv, β3, β5|||
|Genistein||DU145||α2, α3, β1|||
|Valproic acid (VPA)||PC-3||α1, α3||α6, β3||[168,169,170,171]|
|VPA + interferon-α||PC-3||α1, α3, β1||α5, α6, β4|||
|RAD001||PC-3||α2, β3||α1, α5||[70,169,171]|
|AEE877+ RAD001||PC-3||α2, β3||α1, α5|||
|VPA+RAD001||PC-3||α1, α2, α3||α5, α6, β3|||
|VPA+AEE788||PC-3||α1, α3||α5, α6, β3|||
|VPA+AEE788+RAD001||PC-3||α1, α2, α3, β1||α5, α6, β3|||
|VPA||LNCaP||α2, α3, α5, α6, β1||[168,169,170,171]|
|VPA + interferon-α||LNCaP||α2, α3, α5, α6, β1, β3, β4|||
|AEE877+ RAD001||LNCaP||α3, α5, α6, β1|||
|VPA+RAD001||LNCaP||α2, α3, α5, α6, β1|||
|VPA+AEE788||LNCaP||α2, α3, α5, α6, β1|||
|VPA+AEE788+RAD001||LNCaP||α2, α3, α5, α6, β1|||
A number of molecularly targeted drugs with different mechanisms of action have been assessed for their effects on integrin expression as well as on prostate cancer cell growth and motility. The effects of RAD001 (mTOR inhibitor), VPA (HDAC inhibitor), and AEE877 (EGFR/VEGFR inhibitor) have been extensively investigated by Wedel et al. [70,168,169,170,171]. These drugs inhibited adhesion of all cell lines tested, and inhibited migration and invasion of β3-expressing PC-3 cells but had lesser effect on β3 negative LNCaP cells [70,169]. Changes in integrin expression and localisation in response to treatment were cell line dependent; β3 and β4 were often downregulated in cell lines that originally expressed them, and β4 was relocated from the cell surface to an intracellular distribution in PC-3 cells [168,169,170,171]. Initial expression of the β3 and β4 subunits was proposed as a biomarker predicting response to treatment. Changes in expression of β3 and β1 and associated α subunits including α5 may promote anti-invasive and anti-metastatic effects. RAD001 alone was a less effective anti-adhesive agent than the other compounds tested and was found to upregulate β3, suggesting potential issues with RAD001 promoting drug resistance and metastasis .
Camptothecin, and the anti-angiogenic camptothecin-somatostatin conjugate JF-10-81, reduced expression of functional αvβ3 and αvβ5 on PC-3 cells, as well as reducing PI3K/Akt signalling and expression of MMP-2 and MMP-9 . These results suggest that the anti-migration and anti-invasive effects of camptothecin may result from interference with integrin-mediated signalling. Further work is required to determine the mechanism by which camptothecin downregulates αvβ3/αvβ5 expression.
Exposure of LNCaP cells to androgen stimulation and radiation resulted in increased cell surface expression of the αv and β1 integrin subunits resulting in increased adhesion to fibronectin, which may provide a mechanism for radioresistance . In patients with locally advanced disease, the anti-androgen bicalutamide adjuvant to radiotherapy demonstrates significant clinical benefits in terms of overall survival, PFS and PSA-PFS compared with radiotherapy alone . Since bicalutamide normalized integrin expression, this could provide a mechanism by which this agent increases tumour radiosensitivity. Furthermore, use of an integrin antagonist could provide beneficial anti-adhesion effects without the side-effects and risk of promoting androgen independent disease seen with hormone therapy.
Addition of physiological levels of fish oil, lycopene, or vitamin E to cell culture medium decreased cell surface expression of αvβ3 and αvβ5 on PC-3 cells . The role of vitamin E in prostate cancer is complex since it has been shown to promote tumour growth, however lycopene and fish oil have been linked with decreased cancer risk, and may have potential as adjuvant nutrients.
Treatment of PC-3 cells with the α1-adrenoceptor antagonist doxazosin caused a significant decrease in the levels of α5, αv, β1, β5 and β6 mRNA, accompanied by an increase in β8 . Doxazosin induced apoptosis, possibly by interfering with cell attachment and integrin mediated survival signalling.
Changes in integrin expression in response to chemotherapy can be exploited to increase response to anti-integrin therapeutics . However, changes in expression could also render a targeted therapeutic ineffective therefore incorporation of an integrin antagonist into existing or novel drug combinations will require prior assessment of their effects on integrin expression at the preclinical stage. Measurement of changes in integrin expression may also prove useful as biomarkers, for example, in detecting upregulation of pro-tumourigenic integrins as an indicator of the limit of clinical effectiveness of a particular therapy, or the development of resistance.
5. RGD-Binding Integrin Antagonists in Prostate Cancer Therapy
5.1. Preclinical Studies
Overexpression of integrins αvβ3, αvβ5 and α5β1 on tumour tissue and angiogenic vasculature, and the associated consequences on tumour progression and/or resistance, makes them attractive targets for molecularly targeted therapy. In addition, involvement of osteoclast αvβ3 in bone remodelling provides further incentive for developing αvβ3 antagonists as novel therapies for osteoporosis and bone metastasis. In recognition of the importance of the integrins in promoting cancer progression, integrin antagonist antibodies, peptides and small molecules have been developed for a wide range of anti-tumour applications. Here, we review antagonists of RGD-binding integrins specifically relevant to prostate cancer.
The importance of targeting integrins in the tumour microenvironment including on endothelial cells in addition to those on tumour cells was demonstrated using αvβ3/αIIbβ3 negative PC-3 cells grown in an αvβ3-positive bone microenvironment . Antagonism of β3 integrins with m7E3 F(ab')2 reduced tumour growth, angiogenesis, and tumour-induced bone degradation. This demonstrated that targeting RGD-binding integrins, specifically the β3 subfamily will have therapeutic benefit against bone metastasis regardless of tumour integrin profile, particularly important in light of recent demonstrations of heterogeneity of genes and receptors expressed by tumour cells within single patients .
Integrin knockdown has confirmed the importance of targeting integrin-mediated interactions with the microenvironment and that the αv integrin subfamily is a target for the prevention and treatment of bone metastasis. In studies progressing the development of GLPG0187, knockdown of αv in PC-3 cells reduced clonogenicity and prevented the growth of subcutaneous tumour xenografts in vivo . A gene therapy approach using liposomes to deliver αv-siRNA to established tumours showed that αv-knockdown had no effect on the growth of established subcutaneous PC-3 xenografts, but significantly decreased the growth of PC-3 xenografts in bone and associated bone destruction, indicating αv integrin mediated communication with the bone microenvironment is required for the development of bone metastases .
The small molecule multi-integrin antagonist GLPG0187 has been reported to have a low nanomolar affinity for RGD-recognising integrins, with the exception of αIIbβ3 . GLPG0187 is a potent anti-angiogenic and anti-osteoporotic agent, and inhibits in vitro adhesion and migration of prostate cancer cell lines. GLPG0187 treatment caused an increase in the E-cadherin/vimentin ratio, indicating a switch to an epithelial phenotype, and caused a decrease in the subpopulation of prostate cancer stem cells. In vivo, dosing with GLPG0187 reduced the tumour burden developed in mice injected intracardiacally with PC-3M-Pro4/luc cells as a model of the development of new bone metastases. Treatment of mice with established bone metastases significantly reduced the growth of existing tumours and prevented the formation of new metastases [148,178]. These studies identify GLPG0187 as a promising treatment for metastatic prostate cancer, and it has been shown to be safe in healthy volunteers. A Phase 1b trial in advanced solid tumours is currently in progress .
The small molecule integrin antagonist S247 has been described as an αvβ3 antagonist, but actually has low nanomolar affinity for all αv integrins . S247 has been effective at preventing bone metastasis in mouse models of breast cancer [180,181], and has been investigated as a combination therapy with radiation, to combat the increase in pro-survival integrin signalling occurring when αvβ3 is upregulated in response to radiation . A PC-3/HUVEC co-culture model was used to model tumour-endothelium interactions; S247 and radiation showed an enhanced reduction in cell proliferation and survival compared to single therapies, and prevented HUVEC migration induced by irradiation of PC-3 cells. In a PC-3 xenograft model, monotherapy with S247 significantly reduced tumour growth, and combination with radiotherapy resulted in a greater delay in tumour growth and, in some cases, caused tumour regression .
The Ac-PHSCN-NH2 peptide, ATN-161, was developed as an antagonist of the PHSRN synergy sequence of fibronectin which acts as a promoter of DU145 cancer cell invasion through binding to α5β1 . PHSCN covalently binds the β subunits of α5β1, αvβ3 and αvβ5 by forming disulfide crosslinks with the integrin  and prevents cancer cell invasion independent of effects on adhesion. ATN-161 was a nanomolar inhibitor of α5β1-expressing MLL prostate cancer cell invasion in vitro, and effectively suppressed tumour growth, angiogenesis and metastasis in vivo . Notably, ATN-161 suppressed the development of metastases when treatment was started after resection of primary MLL tumours , a model highly relevant to adjuvant treatment in the clinic. Treatment started prior to the removal of DU145 tumours completely prevented recurrence of the primary tumour and significantly reduced metastasis . A polylysine dendrimer, Ac-PHSCNGGK-MAP, bearing 8 PHSCN peptides has been shown to be significantly more potent than ATN-161, with synergistic enhancements of inhibition of fibronectin-induced invasion of PC-3 or DU145 cells in vitro, and inhibition of cell extravasation and development of micrometastases in the lungs in vivo  To date, studies on PHSCN peptides have focussed on combating lung metastasis. Investigation of PHSCN peptides effects on bone metastasis will be important in determining whether they will prove to be an effective general treatment for advanced disease. ATN-161 has been progressed to phase 1 clinical trials including 4 patients with prostate carcinoma; it was well-tolerated and one patient with prostate cancer experienced prolonged stabilisation of disease .
Disintegrins are a family of small peptide integrin antagonists derived from snake venom . Many contain RGD or related motifs and act as antagonists of the RGD-recognising integrins, blocking platelet aggregation and integrin mediated cell adhesion. Other disintegrins contain different integrin binding motifs such as LDV or MLD, permitting them to antagonise other β1 family integrins. The high anti-integrin activity of disintegrins has led to interest in their development as potential anti-thrombotic or anti-metastatic agents. Disintegrins including rhodostomin, triflavin and trigramin have been shown to inhibit αIIbβ3-mediated tumour cell induced platelet aggregation , αvβ3-mediated adhesion of PC-3 cells to the extracellular matrix produced by osteoblasts , and tumour growth in bone , suggesting they may be useful as a treatment for bone metastasis.
The disintegrin contortrostatin has been shown to bind a number of RGD-containing integrins, including αvβ3, αIIbβ3, αvβ5 and α5β1 (Binding Kd: αvβ3 6.6 nM, αvβ5 19.5 nM, α5β1 191.3 nM ), rendering it able to prevent invasion and tumour growth of cancer cells expressing a range of integrin profiles . Contortrostatin inhibited PC-3 cell migration and enhanced the cytotoxicity of docetaxel in vitro . In subcutaneous xenograft models, contortrostatin alone significantly decreased growth of both androgen dependent (CWR-22) and independent (PC-3) tumours, and an increased effect was obtained in combination with docetaxel. Contortrostatin and combination therapy was also effective at preventing the growth of orthotopic bone lesions from PC-3. Further investigations are required to determine whether the enhanced efficacy of docetaxel with contortrostatin is simply additive or whether there is a synergistic effect. Nevertheless, the observation that disintegrins can both enhance the effect of a currently used chemotherapy drug and affect the development of bone metastasis, strongly encourages further research on these proteins. The availability of recombinant disintegrins [190,192] provides a substantial translational potential for their use as biopharmaceuticals; they are now readily produced in large quantities, and mutated variants can be produced to explore integrin affinity and antitumour efficacy.
5.2. Clinical Trials
The cyclic peptide cRGDfV has been shown to be cytotoxic to αvβ3-expressing prostate cancer cells, inducing apoptosis by interference with integrin-mediated FAK signalling . No effects were observed on cells which did not express αvβ3, therefore cRGDfV was proposed as a non-toxic targeted therapy for prostate cancer. The most advanced integrin antagonist in clinical development, cilengitide, is a N-methylated cRGDfV derivative, c(RGDf(NMe)V) with high affinity for αvβ3, αvβ5 and α5β1 [193,194,195]. In vitro, cilengitide has been shown to be highly effective at blocking cell adhesion and migration mediated by αvβ3 and αvβ5 , and induced apoptosis in cancer cells dependent on integrin mediated cell adhesion for survival . In vivo, cilengitide reduced angiogenesis, tumour growth and tumour metastasis . A preclinical study has shown that low doses of cilengitide have a paradoxical effect promoting tumour growth . Although the relevance of this observation to conditions in the clinic has been questioned , it has been suggested that the dose level or timing of treatment will require careful optimisation.
At least 21 clinical trials involving cilengitide have been completed or are ongoing, the most notable being a current Phase III trial for the treatment of glioblastoma. Phase I studies on advanced solid tumours [200,201] showed cilengitide was safe and well-tolerated. No patients experienced partial or complete response to treatment, although some achieved prolonged stable disease. Subsequently, cilengitide has been tested in two Phase II trials against metastatic and non-metastatic prostate cancer . 13 patients with non-metastatic castration resistant prostate cancer were treated with 2000 mg cilengitide administered intravenously twice weekly . Cilengitide was well tolerated but no patients met the primary endpoint of a decrease in PSA level of ≥50%, indeed 11 experienced an increase in PSA level over the course of the trial, resulting in the conclusion that cilengitide had no detectable activity. 44 patients with asymptomatic metastatic castration resistant prostate cancer were treated with either 500 or 2,000 mg cilengitide administered intravenously twice weekly . Some clinical effect was observed, with 7 patients experiencing stable disease (average duration 9.9 months). All patients had an increase in biomarkers of bone turnover, but this was less pronounced in those receiving the higher dose, and those with stable disease.
MK-0429, a small molecule αvβ3 antagonist, has been investigated in a phase I trial of patients with castration resistant prostate cancer and bone metastases . 21 patients received either 200 mg or 1,600 mg MK-0429 twice daily for 4 weeks. This short duration of treatment made it difficult to draw conclusions about drug efficacy. The drug was safe, well-tolerated, and showed effects on bone turnover with a significant reduction in uNTx, a biomarker of bone resorption. However, all patients had an increase in PSA.
DI17E6, a monoclonal antibody targeting αv integrins  has been investigated as a salvage therapy for patients whose disease progressed on standard chemotherapy. In a phase I trial of patients with castration resistant prostate cancer and bone metastases, 26 patients were treated with DI17E6 every 2 weeks for between 14 and 534 days. Two patients had a significant response with a marked decrease in PSA levels, and pain relief. One of these showed a reduction in size of the primary tumour and lymph nodes [207,208].
Another anti-αv monoclonal antibody, intetumumab (CNTO 95) has been investigated as a combination therapy for metastatic castration resistant prostate cancer. 10 patients were treated with a combination of 5 or 10 mg/kg intetumumab combined with 75 mg/m² docetaxel. 5 mg/kg intetumumab was not effective, whereas no progressive disease occurred in the 10 mg/kg group, with 1 patient having partial tumour response and 4 having a decrease in PSA levels . The anti-αvβ3 antibody etaracizumab (Abegrin, Medi-522) has been investigated in a phase II clinical trial as a treatment for metastatic castration resistant prostate cancer in combination with docetaxel, prednisone, and zoledronic acid . Results from this study have not been reported.
Conclusions on the clinical efficacy of integrin antagonists in prostate cancer are limited due to the small size of all studies conducted so far. Integrin antagonists are generally well-tolerated, and antibodies targeting a range of αv integrins have shown some efficacy against advanced prostate cancer.
6. Integrin Targeting for Drug Delivery and Imaging
The overexpression of integrins, specifically αvβ3, on a wide range of tumour cells and vasculature has made αvβ3 a popular target for the development of agents for tumour detection and drug delivery. The most common strategy is use of a cyclic RGD containing peptide with high affinity to αvβ3, and possibly also αvβ5. The majority of RGD-targeted imaging agents are first developed in models of glioma, since U87MG expresses very high levels of αvβ3 and αvβ5. The wider field of integrin imaging and targeting in cancer has been recently reviewed in Theranostics [210,211]. Here, we discuss approaches specifically targeting prostate cancer.
6.1. Drug Delivery
cRGDfK peptide has been used to direct HPMA-copolymer geldanamycin conjugates to prostate cancer cells and tumour vasculature to avoid the dose-limiting toxicity associated with current therapeutic use of geldanamycin . αvβ3-expressing PC-3, DU145 and HUVEC cells were used for initial assessment of the conjugates’ anticancer activity, showing activity comparable to or greater than the free drug whereas a geldanamycin-free cRGDfK-targeted polymer showed cytotoxic effects at high concentrations. αvβ3-targeted copolymers significantly inhibited HUVEC migration and tube formation  whereas cRGDfK conjugate without geldanamycin or cRGDfK alone had no effect on HUVEC migration. In vivo, αvβ3 targeting of geldanamycin doubled the tolerable dose in nude mice. The conjugate effectively delivered drug to DU145 xenografts, providing an intratumoural drug concentration over 8 times higher than that provided by the equivalently tolerated dose of free geldanamycin . Xenograft growth and in vivo angiogenesis were effectively inhibited. Subtherapeutic doses of αvβ3-targeted conjugate increased tumour tissue permeability, which could be exploited to improve drug delivery of a second agent by use of the cRGDfK targeting agent in a combination therapy .
cRGDfK has also been used as a targeting group to deliver other payloads. HPMA-copolymer docetaxel conjugates have been investigated in models of prostate cancer in order to address toxicity and solubility issues seen with current docetaxel formulations . The copolymers are water soluble and combine active tumour targeting with improved renal elimination of conjugated drug in the systemic circulation. In vivo, αvβ3-targeted copolymer caused no acute toxicity and caused a significantly greater reduction in DU145 xenograft tumour growth compared to docetaxel alone. Nanoparticles containing Pt(IV) prodrugs targeted by cRGDfV are more cytotoxic than cisplatin in PC-3 and DU145 cell lines , but have not yet been tested in in vivo models of prostate cancer, although they proved to be effective in suppressing tumour growth in a breast cancer model. cRGDfV-functionalised gold nanorods bound selectively to αvβ3-expressing cells in vitro, but failed to localise to DU145 tumours in vivo due to rapid clearance .
The RGD4C αvβ3-binding peptide has been used to deliver HPMA-copolymers chelating β-emitting isotopes for tumour-targeted radiotherapy . Imaging of tumour angiogenesis was also possible via chelation of 99mTc and showed specific and enhanced uptake in DU145 and PC3 xenografts [218,219], and prolonged retention of the conjugated isotope . Chelation of 90Y resulted in significant inhibition of DU145 tumour growth with no evidence of radiation injury to non-target organs .
Linear CRGDC has been conjugated to the N-terminus of tachyplesin, a 17 amino acid antimicrobial cyclic peptide produced by the horseshoe crab which triggers apoptosis in cancer cells. RGD-tachyplesin reduced the proliferation and colony formation of TSU prostate cancer cells in vitro, increased cell permeability by causing damage to the plasma membrane, and initiated apoptosis through both Fas-dependent and independent pathways. In vivo, RGD-tachyplesin inhibited TSU xenograft growth in the CAM model without toxicity to the embryo .
The iRGD peptide has been used to promote tumour penetration of imaging agents and drugs. iRGD contains a cyclic RGD motif which first binds to tumour cells via αv integrins then undergoes proteolytic cleavage to reveal a linear CendR fragment with affinity for neuropilin-1 which allows cell and tissue penetration. iRGD has high affinity for αvβ3 and αvβ5 but not α5β1 and is localised to regions overexpressing both αv and neuropilin-1 in 22Rv1 orthotopic prostate cancer xenografts. iRGD-coating of abraxane increased abraxane accumulation in 22Rv1 tumours 8-fold compared to the untargeted drug, whereas CRGDC-targeting only afforded a 2-fold increase, and effectively inhibited tumour growth. Additionally, conjugation to iron oxide nanoworms allowed MRI imaging of the whole tumour region, a significant improvement compared to the linear CRGDC peptide which only targeted tumour vasculature, or untargeted nanoworms which gave no signal in the tumour . Use of iRGD for drug delivery provides a substantial advantage over other integrin-targeting peptides due to its greater tissue penetration promoting capabilities.
A fusion protein containing a linear RGD peptide attached to the Fc fragment of mouse IgG has been used in gene therapy to target αvβ3 expressing tumours, and tumour angiogenesis through αvβ3 expressing endothelial cells . The selectivity of the peptide used for αvβ3 over other RGD-binding integrins was not reported. In vivo, injection of an adenovirus vector causing expression of the fusion protein significantly decreased the growth of DU-145 xenografts, and similar growth suppression was observed in tumours distant from the treated site. The fusion protein can be used as a targeted therapeutic separate from its gene therapy applications, thus may have potential as a treatment of metastatic prostate cancer.
A fibronectin mimetic peptide containing RGDSP and PHSRN sequences has been developed to deliver liposomes  or polymer vesicles  to α5β1-expressing prostate cancer cells. Including the PHSRN sequence directed the peptide specifically to α5β1 and increased efficiency of internalisation and drug delivery to LNCaP cells compared to GRGDSP. α5β1-targeting of polymer vesicles containing TNF-α increased cytotoxicity 2.6-3.8-fold compared to free TNF-α . In vivo applications of this strategy have not yet been reported.
The low metabolic activity observed in prostate cancer has led to 18F-fluorodeoxyglucose-based PET scanning being found unsuitable for diagnosis and staging . Targeting of positron emitting agents to integrin receptors overexpressed on the tumour has been proposed as one method of overcoming this problem . Other PET imaging approaches for the detection and monitoring of primary and metastatic prostate cancer have been reviewed by Cai et al. in 2010 .
Initial investigations of αvβ3-targeted PET imaging in prostate cancer models raised some concerns that the level of αvβ3 on tumour tissue was insufficient to allow good imaging, for example, in a study of an 18F-labelled peptide bearing two cRGDyK targeting groups, PC-3 tumours had a low to moderate level of αvβ3 expression resulting in low tumour uptake . In preliminary clinical studies, 18F-galacto-RGD detected 78% of bone metastases, but uptake was heterogeneous, indicating that αvβ3 levels differ between patients and metastatic sites .
Integrin targeting has been used in dual targeting approaches to enhance tumour binding and uptake. A range of molecules targeting both αvβ3 and the gastrin releasing peptide receptor (GRPR), which is highly expressed on androgen resistant prostate tumours, have been investigated. 18F-FB-BBN-RGD contains cRGDyK and a bombesin (BBN) peptide analogue as targeting groups  and its uptake in PC-3 cells and tumour depended partially on both αvβ3 and GRPR receptors. In xenograft models, a synergistic enhancement of imaging properties was observed: tumour uptake was increased compared to 18F-FB-RGD in PC-3 tumours, and washout from the tumour was decreased as a result of dual receptor binding. 18F-FB-BBN-RGD was also effective at imaging DU145 tumours with low GRPR levels, and showed reduced background signal compared to 18F-FB-RGD. 18F-FB-BBN-RGD comprised a mixture of two inseparable regioisomeric compounds, and was therefore thought unsuitable for clinical development .
The structure of RGD-BBN dually targeted imaging agents has been modified to improve efficiency of the chemical synthesis. 18F-FB-PEG3-Glu-RGD-BBN incorporated a PEG linker between the peptide and 18F label to improve radiolabelling efficiency and hydrophilicity, and was obtained as a single isomer. 18F-FB-PEG3-Glu-RGD-BBN was more effective than a similar molecule bearing 2 cRGDyK motifs at imaging PC-3 tumour xenografts . A symmetrical linker (AEADP) has also been developed to afford a straightforward chemical synthesis yielding a single compound with defined structure . 18F-FB-AEADP-BBN-RGD showed high and rapid uptake, and good tumour-to-normal tissue contrast in PC-3 tumour xenograft-bearing mice. The AEADP linker provides a platform technology to allow the rapid synthesis of agents bearing different receptor-targeting peptides.
Imaging with 64Cu has been investigated using NOTA and DOTA-bearing cRGDyK-BBN tracers . The NOTA-functionalised peptide had better radiochemical labelling, PC-3 tumour uptake and tumour-to-normal tissue ratios than the DOTA-containing variant. Tumour uptake was slower than 18F-FB-PEG3-Glu-RGD-BBN, but 64Cu-NOTA-RGD-BBN allowed imaging over a longer timeframe due to the longer half-life of 64Cu vs 18F. The high selectivity for tumour tissue and long tumour retention time of 64Cu-NOTA-RGD-BBN suggests this agent may be useful for targeting radiotherapy to prostate tumours.
64Cu-NODA-RGD-Glu-6-Ahx-BBN(7-14)NH2 has been developed to overcome the problem of long retention times in the kidney observed with charged Cu-conjugates such as NOTA . 64Cu-NODA-RGD-Glu-6-Ahx-BBN(7-14)NH2 showed high and diagnostically useful tumour uptake and tumour-to-normal tissue ratio allowing high quality imaging of PC-3 tumours over a long time period. The agent showed low affinity for αvβ3 in vitro, suggesting initial tumour binding occurs through GRPR, and binding of the RGD peptide facilitates subsequent tumour accumulation and retention and urinary excretion.
Combined targeting of αvβ3, nucleolin and tenascin-C has been used as an alternative strategy to enhance tumour imaging . The Simultaneously Multiple Aptamers and RGD Targeting (SMART) probe used a RGD peptide to add αvβ3 targeting capability to magnetic nanoparticles functionalised with fluorescent and Gd-chelating groups for multimodal imaging. The SMART molecule had significantly increased binding efficiency to DU145 cells compared to singly targeted nanoparticles, though it is interesting that a RGD-targeted nanoparticle was the most efficient singly targeted agent in this system.
cRGDfK has been used to target PGA-cystamine-Gd-DOTA conjugates for MRI imaging of αvβ3 expression . Quantitative T1 measurement detected high binding of the targeted agent at the periphery of DU145 tumour xenografts.
Cyanoacrylate microbubbles functionalised with RGD peptide have been used in ultrasound image of the response of AT-1 hormone independent Dunning rat prostate tumours to irradiation . αvβ3-targeted microbubbles showed enhanced accumulation in tumours compared to untargeted or nonsense peptide coated microbubbles. Following 12C irradiation, a significant increase in retention of αvβ3-targeted microbubbles was observed, confirming previous reports that αvβ3 is upregulated in response to radiotherapy. This approach has potential for monitoring tumour response to therapy and developing individualised scheduling of drug or radiation delivery. A related use of targeted microbubbles for the detection of prostate cancer has entered clinical trials .
Pseudopeptide small molecules containing the RGD tripeptide sequence cyclised with an azabicycloalkane unit have been conjugated with fluorescein isothiocyanate to afford non-cytotoxic tracers for αvβ3 imaging. The probe molecules showed αvβ3 expression in PC-3 cells in focal contacts on the cell surface, and also located intracellularly, possibly in cytosolic vesicles . Given the number of small molecules reported as anti-integrin agents, there is potential for the rapid development of a large number of small molecule-based integrin imaging agents with wide ranging selectivities in future.
7. Conclusions and Future Prospects
RGD-binding integrins are a promising target for prostate cancer therapy in advanced disease. αvβ3, αvβ5 and α5β1 play key roles in mediating attraction and binding of tumour cells to the bone microenvironment. The presence of both β3 integrins in the microenvironment contributes to tumour progression through endothelial cell and osteoclast αvβ3 supporting tumour angiogenesis and growth in bone and αIIbβ3 supporting haematogenous metastasis. There is increasing evidence for involvement of αvβ6 in tumour progression. Extensive co-operation and redundancy of function between RGD-binding integrins, and the identification of αv as a node in the cell signalling network supporting advanced prostate cancer give a strong case for development of multi-integrin antagonists targeting the RGD-binding subfamily as molecularly targeted treatments for advanced and metastatic prostate cancer. The small molecule antagonist GLPG0876 is likely to provide partial proof of concept for this approach but its low affinity for αIIbβ3 means it will not demonstrate the full potential. The number of selective integrin targeted agents already showing potential for tumour imaging and targeted therapy provides a supply of compounds for use in combination therapies, and templates for development of further compounds.
We thank Prostate Cancer UK (Pilot Grant Sheldrake PA10-01), the EPSRC (Grant no EP/H002626/1 and RC-UK Fellowship to HMS) and Yorkshire Cancer Research (Programme grant to LHP).
Conflict of Interest
The authors declare no conflicts of interest.
- Jemal, A.; Bray, F.; Center, M.M.; Ferlay, J.; Ward, E.; Forman, D. Global Cancer Statistics. CA Cancer J. Clin. 2011, 61, 69–90, doi:10.3322/caac.20107.
- Santos Amaral, T.M.; Macedo, D.; Fernandes, I.; Costa, L. Castration-Resistant Prostate Cancer: Mechanisms, Targets, and Treatment. Prostate Cancer 2012, 2012, 327253.
- Weinfurt, K.P.; Li, Y.; Castel, L.D.; Saad, F.; Timbie, J.W.; Glendenning, G.A.; Schulman, K.A. The significance of skeletal-related events for the health-related quality of life of patients with metastatic prostate cancer. Ann. Oncology 2005, 16, 579–584, doi:10.1093/annonc/mdi122.
- Adamo, V.; Noto, L.; Franchina, T.; Chiofalo, G.; Picciotto, M.; Tosceno, G.; Caristi, N. Emerging targeted therapies for castration-resistant prostate cancer. Front. Endocrin. 2012, 3, 73.
- Barczyk, M.; Carracedo, S.; Gullberg, D. Integrins. Cell Tissue Res. 2010, 339, 269–280.
- Luo, B.-H.; Springer, T.A. Integrin Structures and Conformational Signalling. Curr. Opin. Cell. Biol. 2006, 18, 579–586, doi:10.1016/j.ceb.2006.08.005.
- Shimaoka, M.; Springer, T.A. Therapeutic Antagonists and Conformational Regulation of Integrin Function. Nat. Rev. Drug Discov. 2003, 2, 703–715, doi:10.1038/nrd1174.
- Han, J.; Lim, C.J.; Watanabe, N.; Soriani, A.; Ratnikov, B.; Calderwood, D.A.; Puzon-McLaughlin, W.; Lafuente, E.M.; Boussiotis, V.A.; Shattil, S.J.; et al. Reconstructing and Deconstructing Agonist-Induced Activation of Integrin αIIbβ3. Curr. Biol. 2006, 16, 1796–1806, doi:10.1016/j.cub.2006.08.035.
- Ginsberg, M.H.; Partridge, A.; Shattil, S.J. Integrin Regulation. Curr. Opin. Cell. Biol. 2005, 17, 509–516, doi:10.1016/j.ceb.2005.08.010.
- Humphries, J.D.; Byron, A.; Humphries, M.J. Integrin ligands at a glance. J. Cell Sci. 2006, 119, 3901–3903, doi:10.1242/jcs.03098.
- Hynes, R.O. Integrins: Bidirectional, Allosteric Signaling Machines. Cell 2002, 110, 673–667, doi:10.1016/S0092-8674(02)00971-6.
- Arnaout, M.A.; Mahalingam, B.; Xiong, J.-P. Integrin Structure, Allostery, and Bidirectional Signalling. Annu. Rev. Cell Dev. Biol. 2005, 21, 318–410.
- Xiao, T.; Takagi, J.; Coller, B.S.; Wang, J.-H.; Springer, T.A. Structural Basis for Allostery in Integrins and Binding to Fibrinogen-mimetic Therapeutics. Nature 2004, 432, 59–67, doi:10.1038/nature02976.
- Xiong, J.-P.; Stehle, T.; Diefenbach, B.; Zhang, R.; Dunker, R.; Scott, D.L.; Joachimiak, A.; Goodman, S.L.; Arnaout, M.A. Crystal Structure of the Extracellular Segment of Integrin αvβ3. Science 2001, 294, 339–345.
- Xiong, J.-P.; Stehle, T.; Zhang, R.; Joachimiak, A.; Frech, M.; Goodman, S.L.; Arnaout, M.A. Crystal Structure of the Extracellular Segment of Integrin αvβ3 in Complex with an Arg-Gly-Asp Ligand. Science 2002, 296, 151–155, doi:10.1126/science.1069040.
- Xiong, J.P.; Mahalingham, B.; Alonso, J.L.; Borrelli, L.A.; Rui, X.; Anand, S.; Hyman, B.T.; Rysiok, T.; Müller-Pompalla, D.; Goodman, S.L.; et al. Crystal structure of the complete integrin αvβ3 ectodomain plus an α/β transmembrane fragment. J. Cell Biol. 2009, 186, 589–600, doi:10.1083/jcb.200905085.
- Nagae, M.; Re, S.; Mihara, E.; Nogi, T.; Sugita, Y.; Takagi, J. Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor. J. Cell Biol. 2012, 197, 131–140, doi:10.1083/jcb.201111077.
- Marinelli, L.; Gottschalk, K.E.; Meyer, A.; Novellino, E.; Kessler, H. Human integrin αvβ5: Homology modeling and ligand binding. J. Med. Chem. 2004, 47, 4166–4177, doi:10.1021/jm030635j.
- Marinelli, L.; Meyer, A.; Heckmann, D.; Lavecchia, A.; Novellino, E.; Kessler, H. Ligand Binding Analysis for Human α5β1 Integrin: Strategies for Designing New α5β1 Integrin Antagonists. J. Med. Chem. 2005, 48, 4204–4207, doi:10.1021/jm040224i.
- Marshall, J.F.; Rutherford, D.C.; McCartney, A.C.; Mitjans, F.; Goodman, S.L.; Hart, I.R. αvβ1 is a receptor for vitronectin and fibrinogen, and acts with α5β1 to mediate spreading on fibronectin. J. Cell Sci. 1995, 108, 1227–1238.
- Nishimura, S.L.; Sheppard, D.; Pytela, R. Integrin αvβ8. Interaction with vitronectin and functional divergence of the β8 cytoplasmic domain. J. Biol. Chem. 1994, 269, 28708–28715.
- Cambier, S.; Gline, S.; Mu, D.; Collins, R.; Araya, J.; Dolganov, G.; Einheber, S.; Boudreau, N.; Nishimura, S.L. Integrin αvβ8-mediated activation of transforming growth factor-β by perivascular astrocytes: An angiogenic control switch. Am. J. Pathol. 2005, 166, 1883–1894, doi:10.1016/S0002-9440(10)62497-2.
- Sato, Y.; Uemura, T.; Morimitsu, K.; Sato-Nishiuchi, R.; Manabe, R.; Takagi, J.; Yamada, M.; Sekiguchi, K. Molecular basis of the recognition of nephronectin by integrin α8β1. J. Biol. Chem. 2009, 284, 4524–4536.
- Sheppard, D. Roles of αv integrins in vascular biology and pulmonary pathology. Curr. Opin. Cell Biol. 2004, 16, 552–557, doi:10.1016/j.ceb.2004.06.017.
- Benjamin, J.T.; Gaston, D.C.; Halloran, B.A.; Schnapp, L.M.; Zent, R.; Prince, L.S. The role of integrin α8β1 in fetal lung morphogenesis and injury. Dev. Biol. 2009, 335, 407–417, doi:10.1016/j.ydbio.2009.09.021.
- Bouvard, D.; Brakebusch, C.; Gustafsson, E.; Aszódi, A.; Bengtsson, T.; Fässler, B. Functional Consequences of Integrin Gene Mutations in Mice. Circ. Res. 2001, 89, 211–223, doi:10.1161/hh1501.094874.
- Silva, R.; D’Amico, G.; Hodivala-Dilke, K.M.; Reynolds, L.E. Integrins. The Keys to Unlocking Angiogenesis. Arterioscler. Thromb. Vasc. Biol. 2008, 28, 1703–1713, doi:10.1161/ATVBAHA.108.172015.
- Weis, S.M.; Cheresh, D.A. Tumor angiogenesis: Molecular pathways and therapeutic targets. Nat. Med. 2011, 17, 1359–1370, doi:10.1038/nm.2537.
- Zou, W.; Teitelbaum, S. Integrins, growth factors, and the osteoclast cytoskeleton. Ann. NY Acad. Sci. 2010, 1192, 27–31, doi:10.1111/j.1749-6632.2009.05245.x.
- Schneider, J.G.; Amend, S.H.; Weilbaecher, K.N. Integrins and bone metastasis: Integrating tumor cell and stromal cell interactions. Bone 2011, 48, 54–65, doi:10.1016/j.bone.2010.09.016.
- Bakewell, S.J.; Nestor, P.; Prasad, S.; Tomasson, M.H.; Dowland, N.; Mehrotra, M.; Scarborough, R.M.; Kanter, J.; Abe, K.; Phillips, D.; et al. Platelet and Osteoclast β3 Integrins are Critical for Bone Metastasis. Proc. Natl. Acad. Sci. USA 2003, 100, 14205–14210.
- Goel, H.K.; Li, J.; Kogan, S.; Languino, L.R. Integrins in Prostate Cancer Progression. Endocr. Relat. Cancer 2008, 15, 657–664, doi:10.1677/ERC-08-0019.
- Felding-Habermann, B.; Fransvea, E.; O’Toole, T.E.; Manzuk, L.; Faha, B.; Hensler, M. Involvement of Tumor Cell Integrin αvβ3 in Hematogenous Metastasis of Human Melanoma Cells. Clin. Exp. Metastasis 2002, 19, 427–436.
- Trikha, M.; Raso, E.; Cai, Y.; Fazakas, Z.; Paku, S.; Porter, A.T.; Timar, J.; Honn, K.V. Role of αIIbβ3 Integrin in Prostate Cancer Metastasis. Prostate 1998, 35, 185–192, doi:10.1002/(SICI)1097-0045(19980515)35:3<185::AID-PROS4>3.0.CO;2-G.
- Desgrosellier, J.S.; Cheresh, D.A. Integrins in cancer: Biological implications and therapeutic opportunities. Nat. Rev. Cancer 2010, 10, 9–22, doi:10.1038/nrc2748.
- Slack-Davis, J.K.; Parsons, J.T. Emerging Views of Integrin Signaling: Implications for Prostate Cancer. J. Cell. Biochem. 2004, 91, 41–46.
- Goel, H.; Alam, N.; Johnson, I.N.S.; Languino, L.R. Integrin signaling aberrations in prostate cancer. Am. J. Transl. Res. 2009, 1, 211–220.
- Navdaev, A.; Eble, J.A. Components of Cell-Matrix Linkage as Potential New Markers for Prostate Cancer. Cancers 2011, 3, 883–896, doi:10.3390/cancers3010883.
- Zheng, D.-Q.; Woodard, A.S.; Fornaro, M.; Tallini, G.; Languino, L.R. Prostatic Carcinoma Cell Migration via αvβ3 Integrin is Modulated by a Focal Adhesion Kinase Pathway. Cancer Res. 1999, 99, 1655–1664.
- Van den Hoogen, C.; van der Horst, G.; Cheung, H.; Buijs, J.T.; Pelger, R.C.M.; van der Pluijm, G. Integrin αv Expression Is Required for the Acquisition of a Metastatic Stem/Progenitor Cell Phenotype in Human Prostate Cancer. Am. J. Pathol. 2011, 179, 2559–2568, doi:10.1016/j.ajpath.2011.07.011.
- Nagle, R.B.; Hao, J.; Knox, J.D.; Dalkin, B.L.; Clark, V.; Cress, A.E. Expression of Hemidesmosomal and Extracellular Matrix Proteins by Normal and Malignant Human Prostate Tissue. Am. J. Pathol. 1995, 146, 1498–1507.
- Pontes-Júnior, J.; Reis, S.T.; Neves de Oliveira, L.C.; Sant’Anna, A.C.; Dall’Oglio, M.F.; Antunes, A.A.; Ribeiro-Filho, L.; Carvalho, P.A.; Cury, J.; Srougi, M.; et al. Association Between Integrin Expression and Prognosis in Localized Prostate Cancer. Prostate 2010, 70, 1189–1195, doi:10.1002/pros.21153.
- Pontes-Júnior, J.; Reis, S.T.; Dall’Oglio, M.; de Oliveira, L.C.N.; Cury, J.; Carvalho, P.A.; Ribeiro-Filho, L.A.; Leite, K.R.M.; Srougi, M. Evaluation of the expression of integrins and cell adhesion molecules through tissue microarray in lymph node metastases of prostate cancer. J. Carcinog. 2009, 8, 3–9, doi:10.4103/1477-3163.48453.
- Putz, E.; Witter, K.; Offner, S.; Stosiek, P.; Zippelius, A.; Johnson, J.; Zahn, R.; Reithmüller, G.; Pantel, K. Phenotypic Characteristics of Cell Lines Derived from Disseminated Cancer Cells in Bone Marrow of Patients with Solid Epithelial Tumors: Establishment of Working Models for Human Micrometastases. Cancer Res. 1999, 59, 241–248.
- De, S.; Chen, J.; Narizhneva, N.V.; Heston, W.; Brainard, J.; Sage, E.H.; Byzova, T.V. Molecular Pathway for Cancer Metastasis to Bone. J. Biol. Chem. 2003, 278, 39044–39050.
- Hartstein, M.E.; Grove, A.S.J.; Woog, J.J. The role of the integrin family of adhesion molecules in the development of tumors metastatic to the orbit. Ophthal. Plast. Reconstr. Surg. 1997, 13, 227–238.
- Goodman, S.L.; Grote, H.J.; Wilm, C. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumors. Biology Open 2012, 1, 329–340, doi:10.1242/bio.2012364.
- Azare, J.; Leslie, K.; Al-Ahmadie, H.; Gerald, W.; Weinreb, P.H.; Violette, S.M.; Bromberg, J. Constitutively Activated Stat3 Induces Tumorigenesis and Enhances Cell Motility of Prostate Epithelial Cells through Integrin β6. Mol. Cell. Biol. 2007, 27, 4444–4453.
- Chen, H.J.; Wang, H.; Zhang, L.; Zhang, Y.K.; Wang, G.M. Correlation between integrin subunits α5 and β1 expressions in prostate cancer and its clinical implication. Zhonghua Nan Ke Xue 2006, 12, 148–150.
- Trikha, M.; Timar, J.; Lundy, S.K.; Szekeres, K.; Tang, K.; Grignon, D.; Porter, A.T.; Honn, K.V. Human Prostate Carcinoma Cells Express Functional αIIbβ3 Integrin. Cancer Res. 1996, 56, 5071–5078.
- Trikha, M.; Cai, Y.; Grignon, D.; Honn, K.V. Identification of a Novel Truncated αIIb Integrin. Cancer Res. 1998, 58, 4771–4775.
- Jin, R.; Trikha, M.; Cai, Y.; Grignon, D.; Honn, K.V. A Naturally Occurring Truncated beta3 Integrin in Tumor Cells: Native Anti-Integrin Involved in Tumor Cell Motility. Cancer Biol. Ther. 2007, 6, 1559–1568, doi:10.4161/cbt.6.10.4710.
- Concato, J.; Jain, D.; Uchio, E.; Risch, H.; Li, W.W.; Wells, C.K. Molecular Markers and Death From Prostate Cancer. Ann. Intern. Med 2009, 150, 595–603.
- Murant, S.J.; Handley, J.; Stower, M.J.; Reid, N.; Cussenot, O.; Maitland, N.J. Co-ordinated Changes in Expression of Cell Adhesion Molecules in Prostate Cancer. Eur. J. Cancer 1997, 33, 263–271.
- Gorlov, I.P.; Byun, J.; Gorlova, O.Y.; Aparicio, A.M.; Efstathiou, E.; Logothetis, C.J. Candidate pathways and genes for prostate cancer: A meta-analysis of gene expression data. BMC Med. Genomics 2009, 2, 48, doi:10.1186/1755-8794-2-48.
- Li, J.; Wang, T.; Goel, H.L.; Jiang, Z.; Cai, Y.; Crockett, K.A.; Zhang, J.Z.; Jain, D.; Coonradt, M.; Manes, T.; et al. A Novel Mechanism of Prostate Cancer Growth Mediated by αvβ6 Integrin and Androgen Receptor. In Presented at the First IMPaCT Meeting, Atlanta, GA, USA, 5–8 September 2007.
- Garlick, D.S.; Li, J.; Sansoucy, B.; Wang, T.; Griffith, L.; FitzGerald, T.J.; Butterfield, J.; Charbonneau, B.; Violette, S.M.; Weinreb, P.H.; et al. αvβ6 integrin expression is induced in the POET and Ptenpc−/− mouse models of prostatic inflammation and prostatic adenocarcinoma. Am. J. Transl. Res. 2012, 4, 165–174.
- Bisanz, K.; Yu, J.; Edlund, M.; Spohn, B.; Hung, M.-C.; Chung, L.W.K.; Hsieh, C.-L. Targeting ECM–Integrin Interaction with Liposome-Encapsulated Small Interfering RNAs Inhibits the Growth of Human Prostate Cancer in a Bone Xenograft Imaging Model. Mol. Ther. 2005, 12, 634–643, doi:10.1016/j.ymthe.2005.05.012.
- Lin, E.; Wang, Q.; Swenson, S.; Jadvar, H.; Groshen, S.; Ye, W.; Markland, F.S.; Pinski, J. The Disintegrin Contortrostatin in Combination With Docetaxel Is a Potent Inhibitor of Prostate Cancer In Vitro and In Vivo. Prostate 2010, 70, 1359–1370.
- Taylor, R.M.; Severns, V.; Brown, D.C.; Bisoffi, M.; Sillerud, L.O. Prostate Cancer Targeting Motifs: Expression of αvβ3, Neurotensin Receptor-1, Prostate Specific Membrane Antigen, and Prostate Stem Cell Antigen in Human Prostate Cancer Cell Lines and Xenografts. Prostate 2012, 72, 523–532, doi:10.1002/pros.21454.
- Rokhlin, O.W.; Cohen, M.B. Expression of cellular adhesion molecules on human prostate tumor cell lines. Prostate 1995, 26, 205–212, doi:10.1002/pros.2990260406.
- Pidgeon, G.P.; Tang, K.; Cai, Y.L.; Piasentin, E.; Honn, K.V. Overexpression of Platelet-type 12-Lipoxygenase Promotes Tumor Cell Survival by Enhancing αvβ3 and αvβ5 Integrin Expression. Cancer Res. 2003, 63, 4258–4267.
- Chatterjee, S.; Brite, K.H.; Matsumura, A. Induction of Apoptosis in Integrin-Expressing Human Prostate Cancer Cells by Cyclic Arg-Gly-Asp Peptides. Clin. Cancer Res. 2001, 7, 3006–3011.
- Sun, Y.-X.; Fang, M.; Wang, J.; Cooper, C.R.; Pienta, K.J.; Taichman, R.S. Expression and Activation of αvβ3 Integrins by SDF-1/CXC12 Increases the Aggressiveness of Prostate Cancer Cells. Prostate 2007, 67, 61–73, doi:10.1002/pros.20500.
- Stachurska, A.; Elbanowski, J.; Kowalczyñska, H.M. Role of α5β1 and αvβ3 integrins in relation to adhesion and spreading dynamics of prostate cancer cells interacting with fibronectin under in vitro conditions. Cell Biol. Int. 2012, 36, 883–892.
- Mulgrew, K.; Kinneer, K.; Yao, X.-T.; Ward, B.K.; Damschroder, M.M.; Walsh, B.; Mao, S.-Y.; Gao, C.; Kiener, P.A.; Coats, S.; et al. Direct targeting of αvβ3 integrin on tumor cells with a monoclonal antibody, AbegrinTM. Mol. Cancer Ther. 2006, 5, 3122–3129, doi:10.1158/1535-7163.MCT-06-0356.
- Romanov, V.I.; Goligorsky, M.S. RGD-recognizing integrins mediate interactions of human prostate carcinoma cells with endothelial cells in vitro. Prostate 1999, 39, 108–118, doi:10.1002/(SICI)1097-0045(19990501)39:2<108::AID-PROS5>3.0.CO;2-9.
- Evangelou, A.; Letarte, M.; Marks, A.; Brown, T.J. Androgen Modulation of Adhesion and Antiadhesion Molecules in PC-3 Prostate Cancer Cells Expressing Androgen Receptor. Endocrinology 2002, 143, 3897–3904, doi:10.1210/en.2002-220156.
- Haywood-Reid, P.L.; Zipf, D.R.; Springer, W.R. Quantification of Integrin Subunits on Human Prostatic Cell Lines—Comparison of Nontumorigenic and Tumorigenic Lines. Prostate 1997, 31, 1–8, doi:10.1002/(SICI)1097-0045(19970401)31:1<1::AID-PROS1>3.0.CO;2-S.
- Wedel, S.; Hudak, L.; Seibel, J.-M.; Juengel, E.; Tsaur, I.; Haferkamp, A.; Blaheta, R.A. Combined targeting of the VEGFr/EGFr and the mammalian target of rapamycin (mTOR) signaling pathway delays cell cycle progression and alters adhesion behavior of prostate carcinoma cells. Cancer Lett. 2011, 301, 17–28, doi:10.1016/j.canlet.2010.11.003.
- Engl, T.; Reija, B.; Marian, D.; Blumenberg, C.; Müller, I.; Beecken, W.-D.; Jones, J.; Ringel, E.M.; Bereiter-Hahn, J.; Jonas, D.; et al. CXCR4 Chemokine Receptor Mediates Prostate Tumor Cell Adhesion through α5 and β3 Integrins. Neoplasia 2006, 8, 290–301, doi:10.1593/neo.05694.
- Edlund, M.; Miyamoto, T.; Sikes, R.A.; Ogle, R.; Laurie, G.W.; Farach-Carson, M.C.; Otey, C.A.; Zhau, H.E.; Chung, L.W.K. Integrin Expression and Usage by Prostate Cancer Cell Lines on Laminin Substrata. Cell Growth Differ. 2001, 12, 99–107.
- Zhang, S.; Wang, J.; Bilen, M.A.; Lin, S.-H.; Stupp, S.L.; Satcher, R.L. Modulation of prostate cancer cell gene expression by cell-to-cell contact with bone marrow stromal cells or osteoblasts. Clin. Exp. Metastasis 2009, 26, 993–1004, doi:10.1007/s10585-009-9289-0.
- Koshida, K.; Konaka, H.; Imao, T.; Egawa, M.; Mizokami, A.; Namiki, M. Comparison of two in vivo models for prostate cancer: Orthotopic and intratesticular inoculation of LNCaP or PC-3 cells. Int. J. Urol. 2004, 11, 1114–1121, doi:10.1111/j.1442-2042.2004.00961.x.
- Sheldrake, H.M.; Patterson, L.H. Function and antagonism of β3 integrins in the development of cancer therapy. Curr. Cancer Drug Targets 2009, 9, 519–540, doi:10.2174/156800909788486713.
- Cooper, C.R.; Chay, C.H.; Pienta, K.J. The Role of alpha(v)beta(3) in Prostate Cancer Progression. Neoplasia 2002, 4, 191–194, doi:10.1038/sj.neo.7900224.
- Jennbacken, K.; Gustavsson, H.; Welén, K.; Vallbo, C.; Damber, J.-E. Prostate Cancer Progression into Androgen Independency Is Associated With Alterations in Cell Adhesion and Invasivity. Prostate 2006, 66, 1631–1640, doi:10.1002/pros.20469.
- Zheng, D.-Q.; Woodard, A.S.; Tallini, G.; Languino, L.R. Substrate Specificity of αvβ3 Integrin-mediated Cell Migration and Phosphatidylinositol 3-Kinase/AKT Pathway Activation. J. Biol. Chem. 2000, 275, 24565–24574.
- Goc, A.; Liu, J.; Byzova, T.; Somanath, P.R. Akt1 mediates prostate cancer cell microinvasion and chemotaxis to metastatic stimuli via integrin β3 affinity modulation. Br. J. Cancer 2012, 107, 713–723, doi:10.1038/bjc.2012.295.
- Wang, X.; Ferreira, A.M.; Shao, Q.; Laird, D.W.; Sandig, M. β3 integrins facilitate matrix interactions during transendothelial migration of PC3 prostate tumor cells. Prostate 2005, 63, 65–80, doi:10.1002/pros.20168.
- Hurt, E.M.; Chan, K.S.; Duhagon Serrat, M.A.; Thomas, S.B.; Veenstra, T.D.; Farrar, W.L. Identification of Vitronectin as an Extrinsic Inducer of Cancer Stem Cell Differentiation and Tumor Formation. Stem Cells 2010, 28, 390–398.
- Hullinger, T.G.; McCauley, L.K.; DeJoode, M.L.; Somerman, M.J. Effect of Bone Proteins on Human Prostate Cancer Cell Lines In Vitro. Prostate 1998, 36, 14–22.
- Bellahcène, A.; Castronovo, V.; Ogbureke, K.U.E.; Fisher, L.W.; Fedarko, N.S. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): Multifunctional proteins in cancer. Nature Rev. Cancer 2008, 8, 212–226, doi:10.1038/nrc2345.
- Waltregny, D.; Bellahcène, A.; Van Riet, I.; Fisher, L.W.; Young, M.; Fernandez, P.; Dewé, W.; de Leval, J.; Castronovo, V. Prognostic value of bone sialoprotein expression in clinically localized human prostate cancer. J. Natl. Cancer Inst. 1998, 90, 1000–1008, doi:10.1093/jnci/90.13.1000.
- Fedarko, N.S.; Jain, A.; Karadag, A.; van Eman, M.R.; Fisher, L.W. Elevated serum bone sialoprotein and osteopontin in colon, breast, prostate, and lung cancer. Clin. Cancer Res. 2001, 7, 4060–4066.
- Ramankulov, A.; Lein, M.; Kristiansen, G.; Loening, S.A.; Jung, K.-Y. Plasma osteopontin in comparison with bone markers as indicator of bone metastasis and survival outcome in patients with prostate cancer. Prostate 2007, 67, 330–340, doi:10.1002/pros.20540.
- Hotte, S.J.; Winquist, E.W.; Stitt, L.; Wilson, S.M.; Chambers, A.F. Plasma osteopontin: Associations with survival and metastasis to bone in men with hormone refractory prostate carcinoma. Cancer 2002, 95, 506–512, doi:10.1002/cncr.10709.
- Jain, A.; McKnight, D.A.; Fisher, L.W.; Humphreys, E.B.; Mangold, L.A.; Partin, A.W.; Fedarko, N.S. Small Integrin-Binding Proteins as Serum Markers for Prostate Cancer Detection. Clin. Cancer Res. 2009, 15, 5199–5207, doi:10.1158/1078-0432.CCR-09-0783.
- Angelucci, A.; Festuccia, C.; D’Andrea, G.; Teti, A.; Bologna, M. Osteopontin Modulates Prostate Carcinoma Invasive Capacity through RGD-Dependent Upregulation of Plasminogen Activators. Biol. Chem. 2002, 383, 229–294.
- Desai, B.; Rogers, M.J.; Chellaiah, M.A. Mechanisms of osteopontin and CD44 as metastatic principles in prostate cancer cells. Mol. Cancer 2007, 6, 18, doi:10.1186/1476-4598-6-18.
- Desai, B.; Ma, T.; Chellaiah, M.A. Invadopodia and matrix degradation: A new property of prostate cancer cells during migration and invasion. J. Biol. Chem. 2008, 283, 13856–13866, doi:10.1074/jbc.M709401200.
- Elgavish, A.; Prince, C.; Chang, P.-L.; Lloyd, K.; Lindsey, R.; Reed, R. Osteopontin Stimulates a Subpopulation of Quiescent Human Prostate Epithelial Cells With High Proliferative Potential to Divide In Vitro. Prostate 1998, 35, 83–94, doi:10.1002/(SICI)1097-0045(19980501)35:2<83::AID-PROS1>3.0.CO;2-H.
- Gordon, J.A.R.; Sodek, J.; Hunter, G.K.; Goldberg, H.A. Bone Sialoprotein Stimulates Focal Adhesion-Related Signaling Pathways: Role in Migration and Survival of Breast and Prostate Cancer Cells. J. Cell. Biochem. 2009, 107, 1118–1128, doi:10.1002/jcb.22211.
- McCabe, N.P.; De, S.; Vasanji, A.; Brainard, J.; Byzova, T.V. Prostate Cancer Specific Integrin αvβ3 Modulates Bone Metastatic Growth and Tissue Remodeling. Oncogene 2007, 26, 6238–6243, doi:10.1038/sj.onc.1210429.
- Fritz, V.; Brondello, J.M.; Gordeladze, J.O.; Reseland, J.E.; Bony, C.; Yssel, H.; Noel, D.; Jorgensen, C. Bone-Metastatic Prostate Carcinoma Favors Mesenchymal Stem Cell Differentiation Toward Osteoblasts and Reduces their Osteoclastogenic Potential. J. Cell. Biochem. 2011, 112, 3234–3245, doi:10.1002/jcb.23258.
- Lai, T.-H.; Fong, Y.-C.; Fu, W.-M.; Yang, R.-S.; Tang, C.-H. Osteoblasts-Derived BMP-2 Enhances the Motility of Prostate Cancer Cells via Activation of Integrins. Prostate 2008, 68, 1341–1353, doi:10.1002/pros.20799.
- Josson, S.; Sharp, S.; Sung, S.-Y.; Johnstone, P.A.S.; Aneja, R.; Wang, R.; Gururajan, M.; Turner, T.; Chung, L.W.K.; Yates, C. Tumor-stromal interactions influence radiation sensitivity in epithelial-versus mesenchymal-like prostate cancer cells. J. Oncol. 2010, 2010, 232831.
- Wang, T.; Huang, J.; Alavian, M.R.; Goel, H.; Plescia, J.; Altieri, D.C.; Languino, L.R.; FitzGerald, T.J. Effect of αvβ3 integrin and αvβ6 integrin on radioresistance of prostate cancer cells mediated through regulation of survivin. In Presented at the 2009 ASCO Genitourinary Cancers Symposium, Orlando, FL, USA, 26–28 February 2009. Abstract No. 130.
- Huang, C.-H.; Yu, H.-S.; Lai, T.-Y.; Yeh, Y.-L.; Su, C.-C.; Hsu, H.-H.; Tsai, F.-J.; Tsai, C.-H.; Wu, H.-C.; Tang, C.-H. Leptin increases motility and integrin up-regulation in human prostate cancer cells. J. Cell. Physiol. 2011, 226, 1274–1282, doi:10.1002/jcp.22455.
- Thomas, S.; Chiriva-Internati, M.; Shah, G.V. Calcitonin receptor-stimulated migration of prostate cancer cells is mediated by urokinase receptor-integrin signaling. Clin. Exp. Metastasis 2007, 24, 363–377, doi:10.1007/s10585-007-9073-y.
- Jain, S.; Chakraborty, G.; Raja, R.; Kale, S.; Kundu, G.C. Prostaglandin E2 Regulates Tumor Angiogenesis in Prostate Cancer. Cancer Res. 2008, 68, 7750–7759.
- Singh, S.; Singh, R.; Sharma, P.K.; Singh, U.P.; Rai, S.N.; Chung, L.W.K.; Cooper, C.R.; Novakovic, K.R.; Grizzle, W.E.; Lillard, J.W.J. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett. 2009, 283, 29–35, doi:10.1016/j.canlet.2009.03.022.
- Bandyopadhyay, A.; Raghavan, S. Defining the Role of Integrin αvβ6 in Cancer. Curr. Drug Targets 2009, 10, 645–652.
- Koivisto, L.; Grenman, R.; Heino, J.; Larjava, H. Integrins α5β1, αvβ1, and αvβ6 Collaborate in Squamous Carcinoma Cell Spreading and Migration on Fibronectin. Exp. Cell Res. 2000, 255, 10–17, doi:10.1006/excr.1999.4769.
- Koistinen, P.; Heino, J. The Selective Regulation of αvβ1 Integrin Expression Is Based on the Hierarchical Formation of αV-containing Heterodimers. J. Biol. Chem. 2002, 277, 24835–24841, doi:10.1074/jbc.M203149200.
- Tchaicha, J.H.; Mobley, A.K.; Hossain, M.G.; Aldape, K.D.; McCarty, J.H. A mosaic mouse model of astrocytoma identifies αvβ8 integrin as a negative regulator of tumor angiogenesis. Oncogene 2010, 29, 4460–4472, doi:10.1038/onc.2010.199.
- Cambier, S.; Mu, D.Z.; O’Connell, D.; Boylen, K.; Travis, W.; Liu, W.H.; Broaddus, V.C.; Nishimura, S.L. A role for the integrin αvβ8 in the negative regulation of epithelial cell growth. Cancer Res. 2000, 60, 7084–7093.
- Altieri, D.C.; Languino, L.R.; Lian, J.B.; Stein, J.L.; Leav, I.; van Wijnen, A.J.; Jiang, Z.; Stein, G.S. Prostate cancer regulatory networks. J. Cell. Biochem. 2009, 107, 845–852, doi:10.1002/jcb.22162.
- Buergy, D.; WEnz, F.; Groden, C.; Brockmann, M.A. Tumor-platelet interaction in solid tumors. Int. J. Cancer 2012, 130, 2747–2760, doi:10.1002/ijc.27441.
- Swaim, M.W.; Chiang, H.-S.; Huang, T.-F. Characterisation of Platelet Aggregation Induced by PC-3 Human Prostate Adenocarcinoma Cells and Inhibited by Venom Peptides, Trigramin and Rhodostomin. Eur. J. Cancer 1996, 32A, 715–721.
- Janowska-Wieczorek, A.; Wysoczynski, M.; Kijowski, J.; Marquez-Curtis, L.; Machalinski, B.; Ratajczak, J.; Ratajczak, M.Z. Microvesicles Derived from Activated Platelets Induce Metastasis and Angiogenesis in Lung Cancer. Int. J. Cancer 2005, 113, 752–760, doi:10.1002/ijc.20657.
- Dashevsky, O.; Varon, D.; Brill, A. Platelet-derived microparticles promote invasiveness of prostate cancer cells via upregulation of MMP-2 production. Int. J. Cancer 2009, 124, 1773–1777, doi:10.1002/ijc.24016.
- Goel, H.L.; Underwood, J.M.; Nickerson, J.A.; Hsieh, C.-C.; Languino, L.R. 1 Integrins Mediate Cell Proliferation in Three-Dimensional Cultures by Regulating Expression of the Sonic Hedgehog Effector Protein, GLI1. J. Cell. Physiol. 2010, 224, 210–217.
- Thomas, F.; Holly, J.M.P.; Persad, R.; Bahl, A.; Perks, C.M. Fibronectin confers survival against chemotherapeutic agents but not against radiotherapy in DU145 prostate cancer cells: Involvement of the insulin like growth factor-1 receptor. Prostate 2010, 70, 856–865.
- Simon, E.L.; Goel, H.L.; Teider, N.; Wang, T.; Languino, L.R.; FitzGerald, T.J. High Dose Fractionated Ionizing Radiation Inhibits Prostate Cancer Cell Adhesion and β1 Integrin Expression. Prostate 2005, 64, 83–91, doi:10.1002/pros.20227.
- Jia, D.; Entersz, I.; Butler, C.; Foty, R.A. Fibronectin matrix-mediated cohesion suppresses invasion of prostate cancer cells. BMC Cancer 2012, 12, 94:1–94:12.
- Gupton, S.L.; Riquelme, D.; Hughes-Alford, S.K.; Tadros, J.; Rudina, S.S.; Hynes, R.O.; Lauffenburger, D.; Gertler, F.B. Mena binds α5 integrin directly and modulates α5β1 function. J. Cell Biol. 2012, 198, 657–676, doi:10.1083/jcb.201202079.
- Gertler, F.; Condeelis, J. Metastasis: Tumor cells becoming MENAcing. Trends Cell Biol. 2011, 21, 81–90, doi:10.1016/j.tcb.2010.10.001.
- Lehr, J.E.; Pienta, K.J. Preferential Adhesion of Prostate Cancer Cells to a Human Bone Marrow Endothelial Cell Line. J. Natl. Cancer Inst. 1998, 90, 118–123, doi:10.1093/jnci/90.2.118.
- Shen, X.; Falzon, M. PTH-related protein modulates PC-3 prostate cancer cell adhesion and integrin subunit profile. Mol. Cell. Endocrinol. 2003, 199, 165–177, doi:10.1016/S0303-7207(02)00287-3.
- Shen, X.; Falzon, M. Parathyroid hormone-related protein upregulates integrin expression via an intracrine pathway in PC-3 prostate cancer cells. Reg. Pept. 2003, 113, 17–29, doi:10.1016/S0167-0115(02)00293-8.
- Chen, H.J.; Wang, H.; Wang, Q.Y.; Zhang, L.; Wang, G.M. Effect of epidermal growth factor signal pathway on integrin α5β1 in prostate cancer cell line DU145. Zhonghua Wai Ke Za Zhi 2006, 44, 966–969.
- Nagakawa, O.; Akashi, T.; Hayakawa, Y.; Junicho, A.; Koizumi, K.; Fujiuchi, Y.; Furuya, Y.; Matsuda, T.; Fuse, H.; Saiki, I. Differential expression of integrin subunits in DU-145/AR prostate cancer cells. Oncol. Rep. 2004, 12, 837–841.
- Neal, C.L.; Mckeithen, D.; Odero-Marah, V.A. Snail negatively regulates cell adhesion to extracellular matrix and integrin expression via the MAPK pathway in prostate cancer cells. Cell Adh. Migr. 2011, 5, 249–257, doi:10.4161/cam.5.3.15618.
- Haraguchi, M.; Okubo, T.; Miyashita, Y.; Miyamoto, Y.; Hayashi, M.; Crotti, T.N.; McHugh, K.P.; Ozawa, M. Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins. J. Biol. Chem. 2008, 283, 23514–23523.
- Moro, L.; Perlino, E.; Marra, E.; Languino, L.R.; Greco, M. Regulation of β1C and β1A integrin expression in prostate carcinoma cells. J. Biol. Chem. 2004, 279, 1692–1702.
- Perlino, E.; Lovecchio, M.; Vacca, R.A.; Fornaro, M.; Moro, L.; Ditonno, P.; Battaglia, M.; Selvaggi, F.P.; Mastropasqua, M.G.; Bufo, P.; et al. Regulation of mRNA and protein levels of β1 integrin variants in human prostate carcinoma. Am. J. Pathol. 2000, 157, 1727–1734, doi:10.1016/S0002-9440(10)64809-2.
- Fuzio, P.; Lucarelli, G.; Perlino, E.; Battaglia, M.; Bettocchi, C.; Sel Vaggi, F.P.; Ditonno, P. Androgen deprivation therapy regulation of β1C integrin expression in prostate cancer. Oncol. Rep. 2009, 22, 327–335.
- Zhu, J.; Motejlek, K.; Wang, D.; Zang, K.; Schmidt, A.; Reichardt, L.F. β8 integrins are required for vascular morphogenesis in mouse embryos. Development 2002, 129, 2891–2903.
- Reynolds, L.E.; Wyder, L.; Lively, J.C.; Taverna, D.; Robinson, S.D.; Huang, X.; Sheppard, D.; Hynes, R.O.; Hodivala-Dilke, K.M. Enhanced Pathological Angiogenesis in Mice Lacking β3 Integrin or β3 and β5 Integrins. Nat. Med. 2002, 8, 27–34, doi:10.1038/nm0102-27.
- Brooks, P.C.; Montgomery, A.M.P.; Rosenfeld, M.; Reisfeld, R.A.; Hu, T.; Klier, G.; Cheresh, D.A. Integrin αvβ3 Antagonists Promote Tumor Regression by Inducing Apoptosis of Angiogenic Blood Vessels. Cell 1994, 79, 1157–1164, doi:10.1016/0092-8674(94)90007-8.
- Nisato, R.E.; Tille, J.C.; Jonczyk, A.; Goodman, S.L.; Pepper, M.S. αvβ3 and αvβ5 integrin antagonists inhibit angiogenesis in vitro. Angiogenesis 2003, 6, 105–119, doi:10.1023/B:AGEN.0000011801.98187.f2.
- Robinson, S.D.; Hodivala-Dilke, K.M. The role of β3-integrins in tumor angiogenesis: Context is everything. Curr. Opin. Cell Biol. 2011, 23, 630–637, doi:10.1016/j.ceb.2011.03.014.
- Van der Flier, A.; Badu-Nkansah, K.; Whittaker, C.A.; Crowley, D.; Bronson, R.T.; Lacy-Hulbert, A.; Hynes, R.O. Endothelial α5 and αv integrins cooperate in remodeling of the vasculature during development. Development 2010, 137, 2439–2449, doi:10.1242/dev.049551.
- Laurens, N.; Engelse, M.A.; Jungerius, C.; Löwik, C.W.; van Hinsbergh, V.W.; Koolwijk, P. Single and combined effects of αvβ3 and α5β1 integrins on capillary tube formation in a human fibrinous matrix. Angiogenesis 2009, 12, 275–285, doi:10.1007/s10456-009-9150-8.
- Dietrich, T.; Onderka, J.; Bock, F.; Kruse, F.E.; Vossmeyer, D.; Stragies, R.; Zahn, G.; Cursiefen, C. Inhibition of inflammatory lymphangiogenesis by integrin α5 blockade. Am. J. Pathol. 2007, 171, 361–372, doi:10.2353/ajpath.2007.060896.
- Margadant, C.; Sonnenberg, A. Integrin—TGF-β crosstalk in fibrosis, cancer and wound healing. EMBO Rep. 2010, 11, 97–105, doi:10.1038/embor.2009.276.
- Naber, H.P.; Wiercinska, E.; Pardali, E.; van Laar, T.; Nirmala, E.; Sundqvist, A.; van Dam, H.; van der Horst, G.; van der Pluijm, G.; Heckmann, B.; et al. BMP-7 inhibits TGF-β-induced invasion of breast cancer cells through inhibition of integrin β3 expression. Cell. Oncol. (Dordr.) 2012, 35, 19–28.
- Bianchi, A.; Gervasi, M.E.; Bakin, A. Role of β5-integrin in epithelial-mesenchymal transition in response to TGF-β. Cell Cycle 2010, 9, 1647–1659, doi:10.4161/cc.9.8.11517.
- Tatler, A.L.; John, A.E.; Jolly, L.; Habgood, A.; Porte, J.; Brightling, C.; Knox, A.J.; Pang, L.; Sheppard, D.; Huang, X.; et al. Integrin αvβ5-mediated TGF-β activation by airway smooth muscle cells in asthma. J. Immunol. 2011, 187, 6094–6107.
- Zambruno, G.; Marchisio, P.C.; Marconi, A.; Vaschieri, C.; Melchiori, A.; Giannetti, A.; de Luca, M. Transforming growth factor-β1 modulates β1 and β5 integrin receptors and induces the de novo expression of the αvβ6 heterodimer in normal human keratinocytes: Implications for wound healing. J. Cell Biol. 1995, 129, 853–865, doi:10.1083/jcb.129.3.853.
- Mythreye, K.; Knelson, E.H.; Gatza, C.E.; Gatza, M.L.; Blobe, G.C. TβRIII/β-arrestin2 regulates integrin α5β1 trafficking, function, and localization in epithelial cells. Oncogene 2012, doi:10.1038/onc.2012.1157.
- Coulson-Thomas, V.J.; Gesteira, T.F.; Coulson-Thomas, Y.M.; Vicente, C.M.; Tersariol, I.L.; Nader, H.B.; Toma, L. Fibroblast and prostate tumor cell cross-talk: Fibroblast differentiation, TGF-β, and extracellular matrix down-regulation. Exp. Cell Res. 2010, 316, 3207–3226, doi:10.1016/j.yexcr.2010.08.005.
- Van der Pluijm, G. Epithelial plasticity, cancer stem cells and bone metastasis formation. Bone 2010, 48, 37–43, doi:10.1016/j.bone.2010.07.023.
- Galliher, A.J.; Schiemann, W.P. β3 Integrin and Src Facilitate Transforming Growth Factor-β Mediated Induction of Epithelial-Mesenchymal Transition in Mammary Epithelial Cells. Breast Cancer Res. 2006, 8, R42:1–R42:16.
- Maschler, S.; Wirl, G.; Spring, H.; Bredow, D.V.; Sordat, I.; Beug, H.; Reichmann, E. Tumor cell invasiveness correlates with changes in integrin expression and localization. Oncogene 2005, 24, 2032–2041, doi:10.1038/sj.onc.1208423.
- Bates, R.C.; Bellovin, D.I.; Brown, C.; Maynard, E.; Wu, B.; Kawakatsu, H.; Sheppard, D.; Oettgen, P.; Mercurio, A.M. Transcriptional activation of integrin β6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma. J. Clin. Invest. 2005, 115, 339–347.
- Van der Horst, G.; van den Hoogen, C.; Buijs, J.T.; Cheung, H.; Bloys, H.; Pelger, R.C.M.; Lorenzon, G.; Heckmann, B.; Feyen, J.; Pujuguet, P.; et al. Targeting of αv-Integrins in Stem/Progenitor Cells and Supportive Microenvironment Impairs Bone Metastasis in Human Prostate Cancer. Neoplasia 2011, 13, 516–525.
- Juárez, P.; Guise, T.A. TGF-β Pathway as a Therapeutic Target in Bone Metastases. Curr. Pharm. Des. 2010, 16, 1301–1312, doi:10.2174/138161210791034049.
- Lang, S.H.; Clarke, N.W.; George, N.J.; Testa, N.G. Primary prostatic epithelial cell binding to human bone marrow stroma and the role of α2β1 integrin. Clin. Exp. Metastasis 1997, 15, 218–227, doi:10.1023/A:1018465213641.
- Rabinovitz, I.; Nagle, R.B.; Cress, A.E. Integrin α6 expression in human prostate carcinoma cells is associated with a migratory and invasive phenotype in vitro and in vivo. Clin. Exp. Metastasis 1995, 13, 481–491, doi:10.1007/BF00118187.
- Cress, A.E.; Rabinovitz, I.; Zhu, W.; Nagle, R.B. The α6β1 and α6β4 integrins in human prostate cancer progression. Cancer Metastasis Rev. 1995, 14, 219–228, doi:10.1007/BF00690293.
- Sroka, I.C.; Sandoval, C.P.; Chopra, H.; Gard, J.M.; Pawar, S.C.; Cress, A.E. Macrophage-dependent cleavage of the laminin receptor α6β1 in prostate cancer. Mol. Cancer Res. 2011, 9, 1319–1328, doi:10.1158/1541-7786.MCR-11-0080.
- Davis, T.L.; Rabinovitz, I.; Futscher, B.W.; Schnölzer, M.; Burger, F.; Liu, Y.; Kulesz-Martin, M.; Cress, A.E. Identification of a novel structural variant of the α6 integrin. J. Biol. Chem. 2001, 276, 26099–26106.
- Pawar, S.C.; Demetriou, M.C.; Nagle, R.B.; Bowden, G.T.; Cress, A.E. Integrin α6 cleavage: A novel modification to modulate cell migration. Exp. Cell Res. 2007, 313, 1080–1089, doi:10.1016/j.yexcr.2007.01.006.
- Ports, M.O.; Nagle, R.B.; Pond, G.D.; Cress, A.E. Extracellular engagement of α6 integrin inhibited urokinase-type plasminogen activator-mediated cleavage and delayed human prostate bone metastasis. Cancer Res. 2009, 69, 5007–5014.
- King, T.E.; Pawar, S.C.; Majuta, L.; Sroka, I.C.; Wynn, D.; Demetriou, M.C.; Nagle, R.B.; Porreca, F.; Cress, A.E. The role of α6 integrin in prostate cancer migration and bone pain in a novel xenograft model. PLoS One 2008, 3, e3535.
- Retta, S.F.; Cassarà, G.; D’Amato, M.; Alessandro, R.; Pellegrino, M.; Degani, S.; de Leo, G.; Silengo, L.; Tarone, G. Cross talk between β1 and αv integrins: β1 affects β3 mRNA stability. Mol. Biol. Cell 2001, 12, 3126–3138.
- Vial, D.; McKeown-Longo, P.J. PAI1 stimulates assembly of the fibronectin matrix in osteosarcoma cells through crosstalk between the αvβ5 and α5β1 integrins. J. Cell Sci. 2008, 121, 1661–1670, doi:10.1242/jcs.020149.
- Defilles, C.; Lissitzky, J.-C.; Montero, M.-P.; André, F.; Prévot, C.; Delamarre, E.; Marrakchi, N.; Luis, J.; Rigot, V. αvβ5/β6 integrin suppression leads to a stimulation of α2β1 dependent cell migration resistant to PI3K/Akt inhibition. Exp. Cell Res. 2009, 315, 1640–1849.
- Defilles, C.; Montero, M.-P.; Lissitzky, J.-C.; Rome, S.; Siret, C.; Luis, J.; Andre, F.; Rigot, V. αv integrin processing interferes with the crosstalk between αvβ5/β6 and α2β1 integrins. Biol. Cell 2011, 103, 519–529, doi:10.1042/BC20100147.
- Gonzalez, A.M.; Gonzales, M.; Herron, G.S.; Nagavarapu, U.; Hopkinson, S.B.; Tsuruta, D.; Jones, J.C.R. Complex interactions between the laminin α4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo. Proc. Natl. Acad. Sci. USA 2002, 99, 16075–16080.
- Alghisi, G.C.; Ponsonnet, L.; Rüegg, C. The Integrin Antagonist Cilengitide Activates αvβ3, Disrupts VE-Cadherin Localization at Cell Junctions and Enhances Permeability in Endothelial Cells. PLoS One 2009, 4, e4449.
- Mani, J.; Vallo, S.; Barth, K.; Makarevic, J.; Juengel, E.; Bartsch, G.; Wiesner, C.; Haferkamp, A.; Blaheta, R.A. Zoledronic acid influences growth, migration and invasive activity of prostate cancer cells In Vitro. Prostate Cancer Prostatic Dis. 2012, 15, 250–255, doi:10.1038/pcan.2012.9.
- Garrison, J.B.; Kyprianou, N. Doxazosin Induces Apoptosis of Benign and Malignant Prostate Cells via a Death Receptor—Mediated Pathway. Cancer Res. 2006, 66, 464–472, doi:10.1158/0008-5472.CAN-05-2039.
- Sun, L.-C.; Luo, J.; Mackey, L.V.; Fuselier, J.A.; Coy, D.H. A conjugate of camptothecin and a somatostatin analog against prostate cancer cell invasion via a possible signaling pathway involving PI3K/Akt, αvβ3/αvβ5 and MMP-2/-9. Cancer Lett. 2007, 246, 157–166, doi:10.1016/j.canlet.2006.02.016.
- Skogseth, H.; Holt, R.U.; Larsson, E.; Halgunset, J. Tyrosine kinase inhibitors alter adhesivity of prostatic cancer cells to extracellular matrix components. APMIS 2006, 114, 225–233, doi:10.1111/j.1600-0463.2006.apm_365.x.
- Hudak, L.; Tezeeh, P.; Wedel, S.; Makarevic, J.; Juengel, E.; Tsaur, I.; Bartsch, G.; Wiesner, C.; Haferkamp, A.; Blaheta, R.A. Low Dosed Interferon Alpha Augments the Anti-Tumor Potential of Histone Deacetylase Inhibition on Prostate Cancer Cell Growth and Invasion. Prostate 2012, doi:10.1002/pros.22525.
- Wedel, S.; Hudak, L.; Seibel, J.-M.; Makarevic, J.; Juengel, E.; Tsaur, I.; Wiesner, C.; Haferkamp, A.; Blaheta, R.A. Impact of combined HDAC and mTOR inhibition on adhesion, migration and invasion of prostate cancer cells. Clin. Exp. Metastasis 2011, 28, 479–491, doi:10.1007/s10585-011-9386-8.
- Wedel, S.; Hudak, L.; Seibel, J.-M.; Juengel, E.; Oppermann, E.; Haferkamp, A.; Blaheta, R.A. Critical Analysis of Simultaneous Blockage of Histone Deacetylase and Multiple Receptor Tyrosine Kinase in the Treatmentof Prostate Cancer. Prostate 2011, 71, 722–735, doi:10.1002/pros.21288.
- Wedel, S.; Hudak, L.; Seibel, J.-M.; Makareviæ, J.; Juengel, E.; Tsaur, I.; Waaga-Gasser, A.; Haferkamp, A.; Blaheta, R.A. Molecular targeting of prostate cancer cells by a triple drug combination down-regulates integrin driven adhesion processes, delays cell cycle progression and interferes with the cdk-cyclin axis. BMC Cancer 2011, 11, 375, doi:10.1186/1471-2407-11-375.
- Wang, T.; Alavian, M.R.; Goel, H.L.; Languino, L.R.; FitzGerald, T.J. Bicalutamide Inhibits Androgen-Mediated Adhesion of Prostate Cancer Cells Exposed to Ionizing Radiation. Prostate 2008, 68, 1734–1742, doi:10.1002/pros.20838.
- See, W.A.; Tyrrell, C.J.; CASODEX Early Prostate Cancer Trialists’ Group. The addition of bicalutamide 150 mg to radiotherapy significantly improves overall survival in men with locally advanced prostate cancer. J. Cancer Res. Clin. Oncol. 2006, 132, S7–S16, doi:10.1007/s00432-006-0132-6.
- Bureyko, T.; Hurdle, H.; Metcalfe, J.B.; Clandinin, M.T.; Mazurak, V.C. Reduced growth and integrin expression of prostate cells cultured with lycopene, vitamin E and fish oil In Vitro. Br. J. Nutr. 2009, 101, 990–997, doi:10.1017/S0007114508051684.
- Chang, M.-W.; Lo, J.-M.; Juan, H.-F.; Chang, H.-Y.; Chuang, C.-Y. Combination of RGD Compound and Low-Dose Paclitaxel Induces Apoptosis in Human Glioblastoma Cells. PLoS One 2012, 7, e37935.
- Nemeth, J.A.; Cher, M.L.; Zhou, Z.; Mullins, C.; Bhagat, S.; Trikha, M. Inhibition of αvβ3 Integrin Reduces Angiogenesis, Bone Turnover, and Tumor Cell Proliferation in Experimental Prostate Cancer Bone Metastase. Clin. Exp. Metastasis 2003, 20, 413–420, doi:10.1023/A:1025461507027.
- Gerlinger, M.; Rowan, A.J.; Horswell, S.; Larkin, J.; Endesfelder, D.; Gronroos, E.; Martinez, P.; Matthews, N.; Stewart, A.; Arpey, P.; et al. Intratumor Heterogeneity and Branched Evolution Revealed by Multiregion Sequencing. N. Engl. J. Med. 2012, 366, 883–892.
- Van der Horst, G.; van den Hoogen, C.; Buijs, J.T.; Cheung, H.; Pelger, R.C.M.; Feyen, J.; Pujuguet, P.; Blanque, R.; Clément-Lacroix, P.; van der Pluijm, G. Targeting of αv integrins, a potential marker for tumor-initiating cells, in prostate cancer and bone stroma inhibits bone metastasis formation. Bone 2010, 47, S320.
- Clinicaltrials.gov. Available online: http://www.clinicaltrials.gov/ (accessed on 24 April 2012).
- Shannon, K.E.; Keene, J.L.; Settle, S.L.; Duffin, T.D.; Nickols, M.A.; Westlin, M.; Schroeter, S.; Ruminski, P.G.; Griggs, D.W. Anti-Metastatic Properties of RGD-Peptidomimetic Agents S137 and S247. Clin. Exp. Metastasis 2004, 21, 129–138, doi:10.1023/B:CLIN.0000024764.93092.5f.
- Harms, J.F.; Welch, D.R.; Samant, R.S.; Shevde, L.A.; Miele, M.E.; Babu, G.R.; Goldberg, S.F.; Gilman, V.R.; Sosnowski, D.M.; Campo, D.A.; et al. A Small Molecule Antagonist of the αvβ3 Integrin Suppresses MDA-MB-435 Skeletal Metastasis. Clin. Exp. Metastasis 2004, 21, 119–128, doi:10.1023/B:CLIN.0000024763.69809.64.
- Abdollahi, A.; Griggs, D.W.; Zieher, H.; Roth, A.; Lipson, K.E.; Saffrich, R.; Gröne, H.-J.; Hallahan, D.E.; Reisfeld, R.A.; Debus, J.; et al. Inhibition of αvβ3 Integrin Survival Signaling Enhances Antiangiogenic and Antitumor Effects of Radiotherapy. Clin. Cancer Res. 2005, 11, 6270–6279.
- Livant, D.L.; Brabec, R.C.; Pienta, K.J.; Allen, D.A.; Kurachi, K.; Markwart, S.; Upashyaya, A. Anti-invasive, Antitumorigenic, and Antimetastatic Activities of the PHSCN Sequence in Prostate Carcinoma. Cancer Res. 2000, 60, 309–320.
- Donate, F.; Parry, G.C.; Shaked, Y.; Hensley, H.; Guan, X.; Beck, I.; Tel-Tsur, Z.; Plunkett, M.L.; Manuia, M.; Shaw, D.E.; et al. Pharmacology of the Novel Antiangiogenic Peptide ATN-161 (Ac-PHSCN-NH2): Observation of a U Shaped Dose-Response Curve in Several Preclinical Models of Angiogenesis and Tumor Growth. Clin. Cancer Res. 2008, 14, 2137–2144.
- Van Golen, K.L.; Bao, L.; Brewer, G.J.; Pienta, K.J.; Kamradt, J.M.; Livant, D.L.; Merajver, S.D. Suppression of Tumor Recurrence and Metastasis by a Combination of the PHSCN Sequence and the Antiangiogenic Compound Tetrathiomolybdate in Prostate Carcinoma. Neoplasia 2002, 4, 373–379, doi:10.1038/sj.neo.7900258.
- Yao, H.; Veine, D.M.; Zeng, Z.-Z.; Fay, K.S.; Staszewski, E.D.; Livant, D.L. Increased potency of the PHSCN dendrimer as an inhibitor of human prostate cancer cell invasion, extravasation, and lung colony formation. Clin. Exp. Metastasis 2010, 27, 173–184, doi:10.1007/s10585-010-9316-1.
- Cianfrocca, M.E.; Kimmel, K.A.; Gallo, J.; Cardoso, T.; Brown, M.M.; Hudes, G.; Lewis, N.; Weiner, L.; Lam, G.N.; Brown, S.C.; et al. Phase 1 Trial of the Antiangiogenic Peptide ATN-161 (Ac-PHSCN-NH2), a beta Integrin Antagonist, in Patients with Solid Tumours. Br. J. Cancer 2006, 94, 1621–1626.
- Calvete, J.J.; Marcinkiewicz, C.; Monleón, D.; Esteve, V.; Celda, B.; Juárez, P.; Sanz, L. Snake venom disintegrins: Evolution of structure and function. Toxicon 2005, 45, 1063–1074, doi:10.1016/j.toxicon.2005.02.024.
- Yang, R.-S.; Tang, C.-H.; Chuang, W.-J.; Huang, T.-H.; Peng, H.-C.; Huang, T.-F.; Fu, W.-M. Inhibition of tumor formation by snake venom disintegrin. Toxicon 2005, 45, 661–669, doi:10.1016/j.toxicon.2005.01.013.
- Minea, R.; Helchowski, C.; Rubino, B.; Brodmann, K.; Swenson, S.; Markland, F., Jr. Development of a chimeric recombinant disintegrin as a cost-effective anti-cancer agent with promising translational potential. Toxicon 2012, 59, 472–486, doi:10.1016/j.toxicon.2011.02.020.
- Swenson, S.; Costa, F.; Ernst, W.; Fujii, G.; Markland, F.S. Contortrostatin, a Snake Venom Disintegrin with Anti-Angiogenic and Anti-Tumor Activity. Pathophysiol. Haemost. Thromb. 2005, 34, 169–176, doi:10.1159/000092418.
- Minea, R.; Swenson, S.; Costa, F.; Chen, T.C.; Markland, F.S. Development of a Novel Recombinant Disintegrin, Contortrostatin, as an Effective Anti-Tumor and Anti-Angiogenic Agent. Pathophysiol. Haemost. Thromb. 2005, 34, 177–183, doi:10.1159/000092419.
- Mas-Moruno, C.; Beck, J.G.; Doedens, L.; Frank, A.O.; Marinelli, L.; Cosconati, S.; Novellino, E.; Kessler, H. Increasing αvβ3 Selectivity of the Anti-Angiogenic Drug Cilengitide by N-Methylation. Angew. Chem. Int. Ed. Engl. 2011, 50, 9496–9500.
- Mas-Moruno, C.; Rechenmacher, F.; Kessler, H. Cilengitide: The First Anti-Angiogenic Small Molecule Drug Candidate. Design, Synthesis and Clinical Evaluation. Anticancer Agents Med. Chem. 2010, 10, 753–768.
- Reardon, D.A.; Nabors, L.B.; Stupp, R.; Mikkelsen, T. Cilengitide: An integrin-targeting arginine-glycine-aspartic acid peptide with promising activity for glioblastoma multiforme. Expert Opin. Invest. Drugs 2008, 17, 1225–1235, doi:10.1517/13543722.214.171.1245.
- Taga, T.; Suzuki, A.; Gonzalez-Gomez, I.; Gilles, F.H.; Stins, M.; Shimada, H.; Barsky, L.; Weinberg, K.I.; Laug, W.E. αv integrin antagonist EMD121974 induces apoptosis in brain tumor cells growing on vitronectin and tenascin. Int. J. Cancer 2002, 98, 690–697, doi:10.1002/ijc.10265.
- Buerkle, M.A.; Pahernik, S.A.; Sutter, A.; Jonczyk, A.; K., M.; Dellian, M. Inhibition of the αv Integrins with a Cyclic RGD Peptide Impairs Angiogenesis, Growth and Metastasis of Solid Tumours In Vivo. Br. J. Cancer 2002, 86, 788–795, doi:10.1038/sj.bjc.6600141.
- Reynolds, A.R.; Hart, I.R.; Watson, A.R.; Welti, J.C.; Silva, R.G.; Robinson, S.D.; Violante, G.; Gourlaouen, M.; Salih, M.; Jones, M.C.; et al. Stimulation of tumor growth and angiogenesis by low concentrations of RGD-mimetic integrin inhibitors. Nat. Med. 2009, 15, 392–400.
- Weller, M.; Reardon, D.; Nabors, B.; Stupp, R. Will integrin inhibitors have proangiogenic effects in the clinic? Nat. Med. 2009, 15, 726.
- Eskens, F.A.L.M.; Dumez, H.; Hoekstra, R.; Perschl, A.; Brindley, C.; Böttcher, S.; Wynendaele, W.; Drevs, J.; Verweij, J.; Oosterom, A.T. Phase I and pharmacokinetic study of continuous twice weekly intravenous administration of Cilengitide (EMD 121974), a novel inhibitor of the integrins αvβ3 and αvβ5 in patients with advanced solid tumours. Eur. J. Cancer 2003, 39, 917–926, doi:10.1016/S0959-8049(03)00057-1.
- Hariharan, S.; Gustafson, D.; Holden, S.; McConkey, D.; Davis, D.; Morrow, M.; Basche, M.; Gore, L.; Zang, C.; O’Bryant, C.L.; et al. Assessment of the biological and pharmacological effects of the αvβ3 and αvβ5 integrin receptor antagonist, cilengitide (EMD 121974), in patients with advanced solid tumors. Ann. Oncology 2007, 18, 1400–1407.
- Beekman, K.W.; Colevas, A.D.; Cooney, K.; DiPaola, R.; Dunn, R.L.; Gross, M.; Keller, E.T.; Pienta, K.J.; Ryan, C.J.; Smith, D.; et al. Phase II Evaluations of Cilengitide in Asymptomatic Patients with Androgen-Independent Prostate Cancer: Scientific Rationale and Study Design. Clin. Genitourin. Cancer 2006, 4, 299–302, doi:10.3816/CGC.2006.n.012.
- Alva, A.; Slovin, S.; Daignault, S.; Carducci, M.; Dipaola, R.; Pienta, K.; Agus, D.; Cooney, K.; Chen, A.; Smith, D.C.; et al. Phase II study of Cilengitide (EMD 121974, NSC 707544) in patients with non-metastatic castration resistant prostate cancer, NCI-6735. A study by the DOD/PCF prostate cancer clinical trials consortium. 2012.
- Bradley, D.A.; Daignault, S.; Ryan, C.J.; Dipaola, R.S.; Smith, D.C.; Small, E.; Gross, M.E.; Stein, M.N.; Chen, A.; Hussain, M. Cilengitide (EMD 121974, NSC 707544) in asymptomatic metastatic castration resistant prostate cancer patients: A randomized phase II trial by the prostate cancer clinical trials consortium. Invest. New Drugs 2011, 29, 1432–1440, doi:10.1007/s10637-010-9420-8.
- Rosenthal, M.A.; Davidson, P.; Rolland, F.; Campone, M.; Xue, L.; Han, T.H.; Mahta, A.; Berd, Y.; He, W.; Lombardi, A. Evaluation of the safety, pharmacokinetics and treatment effects of an αvβ3 integrin inhibitor on bone turnover and disease activity in men with hormone-refractory prostate cancer and bone metastases. Asia Pac. J. Clin. Oncol. 2010, 6, 42–48, doi:10.1111/j.1743-7563.2009.01266.x.
- Wagner, S.; Rothweiler, F.; Anhorn, M.G.; Sauer, D.; Riemann, I.; Weiss, E.C.; Katsen-Globa, A.; Michaelis, M.; Cinatl, J.J.; Schwartz, D.; et al. Enhanced drug targeting by attachment of an anti αv integrin antibody to doxorubicin loaded human serum albumin nanoparticles. Biomaterials 2010, 31, 2388–2398.
- Wirth, M.; Heidenreich, A.; Gschwend, J.E.; Gil, T.; Zastrow, S.; Laniado, M.; Bernard, L.; Vardar, T.; Uhl, W.; Lannert, H. A multicenter phase I study of DI17E6, a novel de-immunized monoclonal antibody to human av integrins, in progressive castrate-resistant prostate cancer (CRPC) with bone metastases after chemotherapy. J. Clin. Oncol. 2011, 29. Abstract e15060.
- Wirth, M.; Heidenreich, A.; Gschwend, J.E.; Gil, T.; Zastrow, S.; Laniado, M.; Zuehlsdorf, M.; Gerloff, J.; Uhl, W.; Lannert, H. Final analysis: A multicenter phase I study of EMD 525797 (DI17E6), a novel humanized monoclonal antibody to human αv integrins, in progressive castrate-resistant prostate cancer with bone metastases after chemotherapy. J. Clin. Oncol. 2012, 30. Abstract 231.
- Chu, F.M.; Picus, J.; Fracasso, P.M.; Dreicer, R.; Lang, Z.; Foster, B. A phase 1, multicenter, open-label study of the safety of two dose levels of a human monoclonal antibody to human α(v) integrins, intetumumab, in combination with docetaxel and prednisone in patients with castrate-resistant metastatic prostate cancer. Invest. New Drugs 2011, 29, 674–679, doi:10.1007/s10637-010-9388-4.
- Chen, X. Integrin Targeted Imaging and Therapy. Theranostics 2011, 1, 28–29, doi:10.7150/thno/v01p0028.
- Chen, K.; Chen, X. Integrin Targeted Delivery of Chemotherapeutics. Theranostics 2011, 1, 189–200, doi:10.7150/thno/v01p0189.
- Borgman, M.P.; Ray, A.; Kolhatkar, R.B.; Sausville, E.; Burger, A.M.; Ghandehari, H. Targetable HPMA Copolymer-Aminohexylgeldanamycin Conjugates for Prostate Cancer Therapy. Pharm. Res. 2009, 26, 1407–1414, doi:10.1007/s11095-009-9851-0.
- Greish, K.; Ray, A.; Bauer, H.; Larson, N.; Maluin, A.; Pike, D.; Haider, M.; Ghandehari, H. Anticancer and antiangiogenic activity of HPMA copolymer-aminohexylgeldanamycin-RGDfK conjugates for prostate cancer therapy. J. Control. Release 2011, 151, 263–270, doi:10.1016/j.jconrel.2010.12.015.
- Borgman, M.P.; Aras, O.; Geyser-Stoops, S.; Sausville, E.A.; Ghandehari, H. Biodistribution of HPMA Copolymer-Aminohexylgeldanamycin-RGDfK Conjugates for Prostate Cancer Drug Delivery. Mol. Pharm. 2009, 6, 1836–1847, doi:10.1021/mp900134c.
- Ray, A.; Larson, N.K.; Pike, D.B.; Grüner, M.; Naik, S.; Bauer, H.; Malugin, A.; Greish, K.; Ghandehari, H. Comparison of Active and Passive Targeting of Docetaxel for Prostate Cancer Therapy by HPMA copolymer-RGDfK Conjugates. Mol. Pharm. 2011, 8, 1090–1099, doi:10.1021/mp100402n.
- Graf, N.; Bielenberg, D.R.; Kolishetti, N.; Muus, C.; Banyard, J.; Farokhzad, O.C.; Lippard, S.J. αvβ3 Integrin-Targeted PLGA-PEG Nanoparticles for Enhanced Anti-tumor Efficacy of a Pt(IV) Prodrug. ACS Nano 2012, 6, 4530–4539, doi:10.1021/nn301148e.
- Gormley, A.J.; Malugin, A.; Ray, A.; Robinson, R.; Ghandehari, H. Biological evaluation of RGDfK-gold nanorod conjugates for prostate cancer treatment. J. Drug Target. 2011, 19, 915–924, doi:10.3109/1061186X.2011.623701.
- Mitra, A.; Nan, A.; Papadimitriou, J.C.; Ghandehari, H.; Line, B.R. Polymer-peptide conjugates for angiogenesis targeted tumor radiotherapy. Nucl. Med. Biol. 2006, 33, 43–52, doi:10.1016/j.nucmedbio.2005.09.005.
- Line, B.R.; Mitra, A.; Nan, A.; Ghandehari, H. Targeting Tumor Angiogenesis: Comparison of Peptide and Polymer-Peptide Conjugates. J. Nucl. Med. 2005, 46, 1552–1560.
- Chen, Y.; Xu, X.; Hong, S.; Chen, J.; Liu, N.; Underhill, C.B.; Creswell, K.; Zhang, L. RGD-Tachyplesin Inhibits Tumor Growth. Cancer Res. 2001, 61, 2434–2438.
- Sugahara, K.N.; Teesalu, T.; Prakash Karmali, P.; Ramana Kotamraju, V.; Agemy, L.; Girard, O.M.; Hanahan, D.; Mattrey, R.F.; Ruoslahti, E. Tissue-Penetrating Delivery of Compounds and Nanoparticles into Tumors. Cancer Cell 2009, 16, 510–520, doi:10.1016/j.ccr.2009.10.013.
- Liu, J.; Ji, J.; Holmes, L.M.; Burgin, K.E.; Barton, L.B.; Yu, X.; Wagner, T.E.; Wei, Y. Fusion protein from RGD peptide and Fc fragment of mouse immunoglobulin G inhibits angiogenesis in tumor. Cancer Gene Ther. 2004, 11, 363–370, doi:10.1038/sj.cgt.7700707.
- Demirgöz, D.; Garg, A.; Kokkoli, E. PR_b-Targeted PEGylated Liposomes for Prostate Cancer Therapy. Langmuir 2008, 24, 13518–13524.
- Demirgöz, D.; Pangburn, T.O.; Davis, K.P.; Lee, S.; Bates, F.S.; Kokkoli, E. PR_b-targeted delivery of tumor necrosis factor-α by polymersomes for the treatment of prostate cancer. Soft Matter 2009, 5, 2011–2019.
- Powles, T.; Murray, I.; Brock, C.; Oliver, T.; Avril, N. Molecular Positron Emission Tomography and PET/CT Imaging in Urological Malignancies. Eur. Urol. 2007, 51, 1511–1521, doi:10.1016/j.eururo.2007.01.061.
- Beer, A.J.; Lorenzen, S.; Metz, S.; Herrmann, K.; Watzlowik, P.; Wester, H.-J.; Peschel, C.; Lordick, F.; Schwaiger, M. Comparison of Integrin αvβ3 Expression and Glucose Metabolism in Primary and Metastatic Lesions in Cancer Patients: A PET Study Using 18F-Galacto-RGD and 18F-FDG. J. Nucl. Med. 2008, 49, 22–29.
- Hong, H.; Zhang, Y.; Sun, J.; Cai, W. Positron emission tomography imaging of prostate cancer. Amino Acids 2010, 39, 11–27, doi:10.1007/s00726-009-0394-9.
- Zhang, X.; Xiong, Z.; Wu, Y.; Cai, W.; Tseng, J.R.; Gambhir, S.S.; Chen, X. Quantitative PET Imaging of Tumor Integrin αvβ3 Expression with 18F-FRGD2. J. Nucl. Med. 2006, 47, 113–121.
- Beer, A.J.; Eiber, M.; Souvatzoglou, M.; Schwaiger, M.; Krause, B.J. Radionuclide and hybrid imaging of recurrent prostate cancer. Lancet Oncol. 2011, 12, 181–191, doi:10.1016/S1470-2045(10)70103-0.
- Li, Z.-B.; Wu, Z.-L.; Chen, K.; Ryu, E.K.; Chen, X. 18F-Labeled BBN-RGD Heterodimer for Prostate Cancer Imaging. J. Nucl. Med. 2008, 49, 453–461, doi:10.2967/jnumed.107.048009.
- Yan, Y.; Chen, K.; Yang, M.; Sun, X.; Liu, S.; Chen, X. A new 18F-labeled BBN-RGD peptide heterodimer with a symmetric linker for prostate cancer imaging. Amino Acids 2011, 41, 439–447, doi:10.1007/s00726-010-0762-5.
- Liu, Z.; Yan, Y.; Chin, F.T.; Wang, F.; Chen, X. Dual Integrin and Gastrin-Releasing Peptide Receptor Targeted Tumor Imaging Using 18F-labeled PEGylated RGD-Bombesin Heterodimer 18F-FB-PEG3-Glu-RGD-BBN. J. Med. Chem. 2009, 52, 425–432.
- Liu, Z.; Li, Z.-B.; Cao, Q.; Liu, S.; Wang, F.; Chan, X. Small-Animal PET of Tumors with 64Cu-Labeled RGD-Bombesin Heterodimer. J. Nucl. Med. 2009, 50, 1168–1177, doi:10.2967/jnumed.108.061739.
- Jackson, A.B.; Nanda, P.K.; Rold, T.L.; Sieckman, G.L.; Szczodroski, A.F.; Hoffman, T.J.; Chen, X.; Smith, C.J. 64Cu-NO2A-RGD-Glu-6-Ahx-BBN(7-14)NH2: A heterodimeric targeting vector for positron emission tomography imaging of prostate cancer. Nucl. Med. Biol. 2012, 39, 377–387, doi:10.1016/j.nucmedbio.2011.10.004.
- Ko, H.Y.; Choi, K.-J.; Lee, C.H.; Kim, S. A multimodal nanoparticle-based cancer imaging probe simultaneously targeting nucleolin, integrin αvβ3 and tenascin-C proteins. Biomaterials 2011, 32, 1130–1138, doi:10.1016/j.biomaterials.2010.10.034.
- Ke, T.; Jeong, E.-K.; Wang, X.; Feng, Y.; Parker, D.L.; Lu, Z.-R. RGD targeted poly(L-glutamic acid)-cystamine-(Gd-DO3A) conjugate for detecting angiogenesis biomarker αvβ3 integrin with MR T1 mapping. Int. J. Nanomed. 2007, 2, 191–199.
- Palmowski, M.; Peschke, P.; Huppert, J.; Hauff, P.; Reinhardt, M.; Maurer, M.; Karger, C.P.; Scholz, M.; Semmler, W.; Huber, P.E.; et al. Molecular Ultrasound Imaging of Early Vascular Response in Prostate Tumors Irradiated with Carbon Ions. Neoplasia 2009, 11, 856–863.
- Kiessling, F.; Fokong, S.; Koczera, P.; Lederle, W.; Lammers, T. Ultrasound Microbubbles for Molecular Diagnosis, Therapy, and Theranostics. J. Nucl. Med. 2012, 53, 345–348, doi:10.2967/jnumed.111.099754.
- Arosio, D.; Manzoni, L.; Araldi, E.M.V.; Caprini, A.; Monferini, E.; Scolastico, C. Functionalized Cyclic RGD Peptidomimetics: Conjugable ligands for αvβ3 Receptor Imaging. Bioconj. Chem. 2009, 20, 1611–1617, doi:10.1021/bc900155j.
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