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Cancers 2011, 3(3), 2990-3001; doi:10.3390/cancers3032990
Article

β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells

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Received: 9 June 2011; in revised form: 18 July 2011 / Accepted: 19 July 2011 / Published: 22 July 2011
(This article belongs to the Special Issue Cancer Signaling Pathways and Crosstalk)
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Abstract: Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression.
Keywords: β-catenin; estrogen receptor; cross-talk; gene expression; transcriptional activity; breast cancer cell β-catenin; estrogen receptor; cross-talk; gene expression; transcriptional activity; breast cancer cell
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gupta, N.; Schmitt, F.; Grebhardt, S.; Mayer, D. β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells. Cancers 2011, 3, 2990-3001.

AMA Style

Gupta N, Schmitt F, Grebhardt S, Mayer D. β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells. Cancers. 2011; 3(3):2990-3001.

Chicago/Turabian Style

Gupta, Nibedita; Schmitt, Fee; Grebhardt, Sina; Mayer, Doris. 2011. "β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells." Cancers 3, no. 3: 2990-3001.



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