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Micromachines 2018, 9(9), 460; https://doi.org/10.3390/mi9090460

Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM)

1
Department of Nano-Physics, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea
2
Gachon Bionano Research Institute, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea
3
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea
4
Laser & Opto-electronics Team, Korea Electronics Technology Institute, Seongnam-si, Gyeonggi-do 13509, Korea
5
Department of Chemical and Bio Engineering, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea
6
Neuroscience Institute, Gil Hospital, Incheon 405-760, Korea
*
Author to whom correspondence should be addressed.
Received: 20 August 2018 / Revised: 6 September 2018 / Accepted: 10 September 2018 / Published: 12 September 2018
(This article belongs to the Special Issue NANO KOREA 2018)
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Abstract

We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation ~ 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules. View Full-Text
Keywords: fluorescence enhancement; surface plasmon resonance; 5-carboxyfluorescein; nano-biophotonics; optical biosensors fluorescence enhancement; surface plasmon resonance; 5-carboxyfluorescein; nano-biophotonics; optical biosensors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tran, N.H.T.; Trinh, K.T.L.; Lee, J.-H.; Yoon, W.J.; Ju, H. Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM). Micromachines 2018, 9, 460.

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