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Micromachines 2018, 9(2), 48; https://doi.org/10.3390/mi9020048

Analysis of PCR Kinetics inside a Microfluidic DNA Amplification System

Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, 1 Shuefu Road, Neipu, Pingtung 91201, Taiwan
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Received: 1 December 2017 / Revised: 18 January 2018 / Accepted: 25 January 2018 / Published: 28 January 2018
(This article belongs to the Special Issue Biomedical Microdevices: Design, Fabrication and Application)
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Abstract

In order to analyze the DNA amplification numerically with integration of the DNA kinetics, three-dimensional simulations, including flow and thermal fields, and one-dimensional polymerase chain reaction (PCR) kinetics are presented. The simulated results are compared with experimental data that have been applied to the operation of a continuous-flow PCR device. Microchannels fabricated by Micro Electro-Mechanical Systems (MEMS) technologies are shown. Comprehensive simulations of the flow and thermal fields and experiments measuring temperatures during thermal cycling are presented first. The resultant velocity and temperature profiles from the simulations are introduced to the mathematical models of PCR kinetics. Then kinetic equations are utilized to determine the evolution of the species concentrations inside the DNA mixture along the microchannel. The exponential growth of the double-stranded DNA concentration is investigated numerically with the various operational parameters during PCR. Next a 190-bp segment of Bartonella DNA is amplified to evaluate the PCR performance. The trends of the experimental results and numerical data regarding the DNA amplification are similar. The unique architecture built in this study can be applied to a low-cost portable PCR system in the future. View Full-Text
Keywords: continuous-flow; polymerase chain reaction; DNA kinetics; kinetic equations continuous-flow; polymerase chain reaction; DNA kinetics; kinetic equations
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chen, J.J.; Li, K.T. Analysis of PCR Kinetics inside a Microfluidic DNA Amplification System. Micromachines 2018, 9, 48.

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