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Micromachines 2017, 8(7), 212; doi:10.3390/mi8070212

Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate

1,2
,
1,3,* , 1,4
,
1
and
1,3,*
1
School of Mechatronics Engineering, Harbin Institute of Technology, West Da-zhi Street 92, Harbin 150001, Heilongjiang, China
2
School of Mechatronics Engineering, Qiqihar University, Wenhua Street 42, Qiqihar 161006, Heilongjiang, China
3
State Key Laboratory of Robotics and System, Harbin Institute of Technology, West Da-zhi Street 92, Harbin 150001, Heilongjiang, China
4
School of Electronics and Control Engineering, Chang’an University, Middle-section of Nan’erHuan Road, Xi’an 710064, Shaanxi, China
*
Authors to whom correspondence should be addressed.
Received: 7 June 2017 / Revised: 2 July 2017 / Accepted: 3 July 2017 / Published: 4 July 2017
(This article belongs to the Special Issue Micro/Nano-Chip Electrokinetics, Volume II)
View Full-Text   |   Download PDF [6626 KB, uploaded 5 July 2017]   |  

Abstract

On-chip immuno-sensors are a hot topic in the microfluidic community, which is usually limited by slow diffusion-dominated transport of analytes in confined microchannels. Specifically, the antigen-antibody binding reaction at a functionalized area cannot be provided with enough antigen source near the reaction surface, since a small diffusion flux cannot match with the quick rate of surface reaction, which influences the response time and sensitivity of on-chip heterogeneous immunoassay. In this work, we propose a method to enhance the transportation of biomolecules to the surface of an antibody-immobilized electrode with induce charge electroosmotic (ICEO) convection in a low concentration suspension, so as to improve the binding efficiency of microfluidic heterogeneous immunoassays. The circular stirring fluid motion of ICEO on the surface of a floating gate electrode at the channel bottom accelerates the transport of freely suspended antigen towards the wall-immobilized antibodies. We investigate the dependence of binding efficiency on voltage magnitude and field frequency of the applied alternate current (AC) electrical field. The binding rate yields a factor of 5.4 higher binding for an applied voltage of 4 V at 10 Hz when the Damkohler number is 1000. The proposed microfluidic immuno-sensor technology of a simple electrode structure using ICEO convective fluid flow around floating conductors could offer exciting opportunities for diffusion-limited on-chip bio-microfluidic sensors. View Full-Text
Keywords: heterogeneous immunoassay; induce charge electroosmosis; binding efficiency heterogeneous immunoassay; induce charge electroosmosis; binding efficiency
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Hu, Q.; Ren, Y.; Liu, W.; Tao, Y.; Jiang, H. Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate. Micromachines 2017, 8, 212.

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