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Micromachines 2017, 8(5), 133; doi:10.3390/mi8050133

A Multiplexed Microfluidic Platform for Bone Marker Measurement: A Proof-of-Concept

1
Nanobiotechnology Department, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
2
Center for Microsystems Technology, Imec and Ghent University, Gent-Zwijnaarde 9052, Belgium
3
Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 1411413137, Iran
4
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, Tehran 1417614418, Iran
5
Nanobiotechnology Department, Research Center for New Technology in Life Sciences Engineering, University of Tehran, Tehran 1417614418, Iran
6
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 1411413137, Iran
7
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 1411413137, Iran
8
Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, Ghent 9052, Belgium
9
Unit for Osteoporosis and Metabolic Bone Diseases, Ghent University Hospital, Ghent 9052, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Kwang W. Oh
Received: 21 February 2017 / Revised: 7 April 2017 / Accepted: 19 April 2017 / Published: 25 April 2017
(This article belongs to the Special Issue Biomedical Microfluidic Devices)
View Full-Text   |   Download PDF [1728 KB, uploaded 25 April 2017]   |  

Abstract

In this work, we report a microfluidic platform that can be easily translated into a biomarker diagnostic. This platform integrates microfluidic technology with electrochemical sensing and embodies a reaction/detection chamber to measure serum levels of different biomarkers. Microfabricated Au electrodes encased in a microfluidic chamber are functionalized to immobilize the antibodies, which can selectively capture the corresponding antigen. An oxidative peak is obtained using the chronoamperometry technique at room temperature. The magnitude of the response current varies linearly with the logarithmic concentration of the relative biomarker and, thus, is used to quantify the concentration of the relative biomarker in serum samples. We demonstrated the implementation, feasibility and specificity of this platform (Osteokit) in assaying serum levels of bone turnover markers (BTMs) using osteocalcin (limits of detection (LOD) = 1.94 ng/mL) and collagen type 1 cross-linked C-telopeptide (CTX) (LOD = 1.39 pg/mL). To our knowledge, this is the first such device fabricated to measure BTMs. Our results also showed that the sensitivity of Osteokit is comparable with the current states of art, electrochemiluminescence (ECLIA). View Full-Text
Keywords: biosensor; bone turnover markers; osteoporosis; gold nanoparticles; electrochemistry biosensor; bone turnover markers; osteoporosis; gold nanoparticles; electrochemistry
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MDPI and ACS Style

Khashayar, P.; Amoabediny, G.; Larijani, B.; Hosseini, M.; Verplancke, R.; Schaubroeck, D.; Van Put, S.; Razi, F.; De Keersmaecker, M.; Adriaens, A.; Goemaere, S.; Fiers, T.; Vanfleteren, J. A Multiplexed Microfluidic Platform for Bone Marker Measurement: A Proof-of-Concept. Micromachines 2017, 8, 133.

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