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Micromachines 2017, 8(4), 121; doi:10.3390/mi8040121

Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles

1
College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
2
Department of Electrical Engineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Received: 3 March 2017 / Revised: 30 March 2017 / Accepted: 7 April 2017 / Published: 11 April 2017
(This article belongs to the Special Issue Microdevices and Microsystems for Cell Manipulation)
View Full-Text   |   Download PDF [5536 KB, uploaded 11 April 2017]   |  

Abstract

Localized single cells can be lysed precisely and selectively using microbubbles optothermally generated by microsecond laser pulses. The shear stress from the microstreaming surrounding laser-induced microbubbles and direct contact with the surface of expanding bubbles cause the rupture of targeted cell membranes. High-resolution single-cell lysis is demonstrated: cells adjacent to targeted cells are not lysed. It is also shown that only a portion of the cell membrane can be punctured using this method. Both suspension and adherent cell types can be lysed in this system, and cell manipulation can be integrated for cell–cell interaction studies. View Full-Text
Keywords: cell poration; cell lysis; optothermal; cell manipulation cell poration; cell lysis; optothermal; cell manipulation
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Fan, Q.; Hu, W.; Ohta, A.T. Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles. Micromachines 2017, 8, 121.

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