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Micromachines 2017, 8(2), 58; doi:10.3390/mi8020058

Optical Flow Cell for Measuring Size, Velocity and Composition of Flowing Droplets

1
Faculty of Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, UK
2
Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Andrew J. deMello, Xavier Casadevall i Solvas and Hongrui Jiang
Received: 23 November 2016 / Revised: 21 January 2017 / Accepted: 9 February 2017 / Published: 16 February 2017
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies, Volume II)
View Full-Text   |   Download PDF [2853 KB, uploaded 16 February 2017]   |  

Abstract

Here an optical flow cell with two light paths is reported that can accurately quantify the size and velocity of droplets flowing through a microchannel. The flow cell can measure the time taken for droplets to pass between and through two conjoined light paths, and thereby is capable of measuring the velocities (0.2–5.45 mm/s) and sizes of droplets (length > 0.8 mm). The composition of the droplet can also be accurately quantified via optical absorption measurements. The device has a small footprint and uses low-powered, low-cost components, which make it ideally suited for use in field-deployable and portable analytical devices. View Full-Text
Keywords: droplet microfluidics; absorbance; real-time monitoring; velocity measurement; enzymatic assays; optical detections droplet microfluidics; absorbance; real-time monitoring; velocity measurement; enzymatic assays; optical detections
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hassan, S.-U.; Nightingale, A.M.; Niu, X. Optical Flow Cell for Measuring Size, Velocity and Composition of Flowing Droplets. Micromachines 2017, 8, 58.

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