Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation
AbstractA robust pick and placement operation of a single cell is necessary for efficient sample collection. Detection and manipulation of single cells requires minimum invasiveness. We report a less-invasive method for picking up and placing single cells using optical and electrical observations for robust cell manipulation. We measured the ionic current through a glass pipette during a cell capture and release operation to detect its capture. Trapping a cell on the pipette tip by suction decreased the current and allowed the detection of cell capture within 1 s. A time-series ionic current was sensitive to the location of a cell and effective at detecting a single cell. A time-series ionic current had a higher signal-to-noise ratio than time-series microscope images. Cell membrane integrity was analyzed at the different capturing and voltage conditions. Serum protein coating shows improvement of a cell release from a pipette tip. Measurement of trajectory and distance of a cell reveals that the movement depends on an ejection flow and the flow in a dish. We achieved a pick-up and placement operation for single cells that was compatible with an open-top microwell while performing observations using optical microscopy and measurements using an electrical current. View Full-Text
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Nagai, M.; Kato, K.; Oohara, K.; Shibata, T. Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation. Micromachines 2017, 8, 350.
Nagai M, Kato K, Oohara K, Shibata T. Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation. Micromachines. 2017; 8(12):350.Chicago/Turabian Style
Nagai, Moeto; Kato, Keita; Oohara, Kiyotaka; Shibata, Takayuki. 2017. "Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation." Micromachines 8, no. 12: 350.
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