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Micromachines 2017, 8(12), 350; https://doi.org/10.3390/mi8120350

Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation

Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan
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Received: 22 October 2017 / Revised: 17 November 2017 / Accepted: 28 November 2017 / Published: 30 November 2017
(This article belongs to the Special Issue Micro/Nano Robotics, Volume II)
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Abstract

A robust pick and placement operation of a single cell is necessary for efficient sample collection. Detection and manipulation of single cells requires minimum invasiveness. We report a less-invasive method for picking up and placing single cells using optical and electrical observations for robust cell manipulation. We measured the ionic current through a glass pipette during a cell capture and release operation to detect its capture. Trapping a cell on the pipette tip by suction decreased the current and allowed the detection of cell capture within 1 s. A time-series ionic current was sensitive to the location of a cell and effective at detecting a single cell. A time-series ionic current had a higher signal-to-noise ratio than time-series microscope images. Cell membrane integrity was analyzed at the different capturing and voltage conditions. Serum protein coating shows improvement of a cell release from a pipette tip. Measurement of trajectory and distance of a cell reveals that the movement depends on an ejection flow and the flow in a dish. We achieved a pick-up and placement operation for single cells that was compatible with an open-top microwell while performing observations using optical microscopy and measurements using an electrical current. View Full-Text
Keywords: micro-manipulation; single cell; cell manipulation; electrical detection; cell screening; micro-well micro-manipulation; single cell; cell manipulation; electrical detection; cell screening; micro-well
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nagai, M.; Kato, K.; Oohara, K.; Shibata, T. Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation. Micromachines 2017, 8, 350.

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