Next Article in Journal
Characterization of a Laterally Oscillating Microresonator Operating in the Nonlinear Region
Next Article in Special Issue
Microfluidic-Based Multi-Organ Platforms for Drug Discovery
Previous Article in Journal
Application of Robust, Packaged Long-Period Fiber Grating for Strain Measurement
Previous Article in Special Issue
Mimicking the Kidney: A Key Role in Organ-on-Chip Development
Article Menu

Export Article

Open AccessReview
Micromachines 2016, 7(8), 130; doi:10.3390/mi7080130

Organ-Tumor-on-a-Chip for Chemosensitivity Assay: A Critical Review

School of Mechanical Engineering, Sharif University of Technology, 11155-9567 Tehran, Iran
*
Authors to whom correspondence should be addressed.
Academic Editors: Nam-Trung Nguyen and Seyed Ali Mousavi Shaegh
Received: 11 May 2016 / Revised: 22 June 2016 / Accepted: 18 July 2016 / Published: 28 July 2016
View Full-Text   |   Download PDF [4096 KB, uploaded 29 July 2016]   |  

Abstract

With a mortality rate over 580,000 per year, cancer is still one of the leading causes of death worldwide. However, the emerging field of microfluidics can potentially shed light on this puzzling disease. Unique characteristics of microfluidic chips (also known as micro-total analysis system) make them excellent candidates for biological applications. The ex vivo approach of tumor-on-a-chip is becoming an indispensable part of personalized medicine and can replace in vivo animal testing as well as conventional in vitro methods. In tumor-on-a-chip, the complex three-dimensional (3D) nature of malignant tumor is co-cultured on a microfluidic chip and high throughput screening tools to evaluate the efficacy of anticancer drugs are integrated on the same chip. In this article, we critically review the cutting edge advances in this field and mainly categorize each tumor-on-a-chip work based on its primary organ. Specifically, design, fabrication and characterization of tumor microenvironment; cell culture technique; transferring mechanism of cultured cells into the microchip; concentration gradient generators for drug delivery; in vitro screening assays of drug efficacy; and pros and cons of each microfluidic platform used in the recent literature will be discussed separately for the tumor of following organs: (1) Lung; (2) Bone marrow; (3) Brain; (4) Breast; (5) Urinary system (kidney, bladder and prostate); (6) Intestine; and (7) Liver. By comparing these microchips, we intend to demonstrate the unique design considerations of each tumor-on-a-chip based on primary organ, e.g., how microfluidic platform of lung-tumor-on-a-chip may differ from liver-tumor-on-a-chip. In addition, the importance of heart–liver–intestine co-culture with microvasculature in tumor-on-a-chip devices for in vitro chemosensitivity assay will be discussed. Such system would be able to completely evaluate the absorption, distribution, metabolism, excretion and toxicity (ADMET) of anticancer drugs and more realistically recapitulate tumor in vivo-like microenvironment. View Full-Text
Keywords: tumor-on-a-chip; cancer in microfluidics; drug efficacy testing; in vitro assays; concentration gradient generators; microchip cell culture; spheroids; tumor microenvironment tumor-on-a-chip; cancer in microfluidics; drug efficacy testing; in vitro assays; concentration gradient generators; microchip cell culture; spheroids; tumor microenvironment
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kashaninejad, N.; Nikmaneshi, M.R.; Moghadas, H.; Kiyoumarsi Oskouei, A.; Rismanian, M.; Barisam, M.; Saidi, M.S.; Firoozabadi, B. Organ-Tumor-on-a-Chip for Chemosensitivity Assay: A Critical Review. Micromachines 2016, 7, 130.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top