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Micromachines 2016, 7(7), 119; doi:10.3390/mi7070119

Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices

Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK
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Author to whom correspondence should be addressed.
Academic Editor: Yoon-Kyoung Cho
Received: 5 May 2016 / Revised: 1 July 2016 / Accepted: 7 July 2016 / Published: 14 July 2016
(This article belongs to the Special Issue Application of Microfluidic Methodology for the Analysis of DNA)
View Full-Text   |   Download PDF [2175 KB, uploaded 14 July 2016]   |  

Abstract

FTA® paper can be used to protect a variety of biological samples prior to analysis, facilitating ease-of-transport to laboratories or long-term archive storage. The use of FTA® paper as a solid phase eradicates the need to elute the nucleic acids from the matrix prior to DNA amplification, enabling both DNA purification and polymerase chain reaction (PCR)-based DNA amplification to be performed in a single chamber on the microfluidic device. A disc of FTA® paper, containing a biological sample, was placed within the microfluidic device on top of wax-encapsulated DNA amplification reagents. The disc containing the biological sample was then cleaned up using Tris-EDTA (TE) buffer, which was passed over the disc, via electro-osmotic flow, in order to remove any potential inhibitors of downstream processes. DNA amplification was successfully performed (from buccal cells, whole blood and semen) using a Peltier thermal cycling system, whereupon the stored PCR reagents were released during the initial denaturing step due to the wax barrier melting between the FTA® disc and PCR reagents. Such a system offers advantages in terms of a simple sample introduction interface and the ability to process archived samples in an integrated microfluidic environment with minimal risk of contamination. View Full-Text
Keywords: microfluidic; DNA purification; DNA amplification; FTA® paper; electro-osmotic flow microfluidic; DNA purification; DNA amplification; FTA® paper; electro-osmotic flow
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wimbles, R.; Melling, L.M.; Shaw, K.J. Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices. Micromachines 2016, 7, 119.

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