Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices
AbstractFTA® paper can be used to protect a variety of biological samples prior to analysis, facilitating ease-of-transport to laboratories or long-term archive storage. The use of FTA® paper as a solid phase eradicates the need to elute the nucleic acids from the matrix prior to DNA amplification, enabling both DNA purification and polymerase chain reaction (PCR)-based DNA amplification to be performed in a single chamber on the microfluidic device. A disc of FTA® paper, containing a biological sample, was placed within the microfluidic device on top of wax-encapsulated DNA amplification reagents. The disc containing the biological sample was then cleaned up using Tris-EDTA (TE) buffer, which was passed over the disc, via electro-osmotic flow, in order to remove any potential inhibitors of downstream processes. DNA amplification was successfully performed (from buccal cells, whole blood and semen) using a Peltier thermal cycling system, whereupon the stored PCR reagents were released during the initial denaturing step due to the wax barrier melting between the FTA® disc and PCR reagents. Such a system offers advantages in terms of a simple sample introduction interface and the ability to process archived samples in an integrated microfluidic environment with minimal risk of contamination. View Full-Text
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Wimbles, R.; Melling, L.M.; Shaw, K.J. Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices. Micromachines 2016, 7, 119.
Wimbles R, Melling LM, Shaw KJ. Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices. Micromachines. 2016; 7(7):119.Chicago/Turabian Style
Wimbles, Ryan; Melling, Louise M.; Shaw, Kirsty J. 2016. "Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices." Micromachines 7, no. 7: 119.
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