Cell Migration According to Shape of Graphene Oxide Micropatterns
AbstractPhotolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds. View Full-Text
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Description: Video S1: Time-lapse video of L929 fibroblasts on triangle GO micropatterns for 12 hours. Video S2: Time-lapse video of L929 fibroblasts on square GO micropatterns for 12 hours.
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Kim, S.E.; Kim, M.S.; Shin, Y.C.; Eom, S.U.; Lee, J.H.; Shin, D.-M.; Hong, S.W.; Kim, B.; Park, J.-C.; Shin, B.S.; Lim, D.; Han, D.-W. Cell Migration According to Shape of Graphene Oxide Micropatterns. Micromachines 2016, 7, 186.
Kim SE, Kim MS, Shin YC, Eom SU, Lee JH, Shin D-M, Hong SW, Kim B, Park J-C, Shin BS, Lim D, Han D-W. Cell Migration According to Shape of Graphene Oxide Micropatterns. Micromachines. 2016; 7(10):186.Chicago/Turabian Style
Kim, Sung E.; Kim, Min S.; Shin, Yong C.; Eom, Seong U.; Lee, Jong H.; Shin, Dong-Myeong; Hong, Suck W.; Kim, Bongju; Park, Jong-Chul; Shin, Bo S.; Lim, Dohyung; Han, Dong-Wook. 2016. "Cell Migration According to Shape of Graphene Oxide Micropatterns." Micromachines 7, no. 10: 186.
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