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Micromachines 2015, 6(10), 1469-1482; doi:10.3390/mi6101431

Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysisand Recovery

Department of Pharmaceutical Sciences, Department of Biomedical Engineering, Sue and Bill Gross Stem Cell Research Center, Chao Family Comprehensive Cancer Center, Edwards Lifesciences Center for Advance Cardiovascular Technology, University of California–Irvine, 845 Health Sciences Road, Irvine, CA 92697, USA
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Academic Editors: Andrew deMello and Xavier Casadevall i Solvas
Received: 17 August 2015 / Revised: 22 September 2015 / Accepted: 25 September 2015 / Published: 30 September 2015
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies)
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Abstract

We describe the design, fabrication and use of a dual-layered microfluidic device for ultrahigh-throughput droplet trapping, analysis, and recovery using droplet buoyancy. To demonstrate the utility of this device for digital quantification of analytes, we quantify the number of droplets, which contain a β-galactosidase-conjugated bead among more than 100,000 immobilized droplets. In addition, we demonstrate that this device can be used for droplet clustering and real-time analysis by clustering several droplets together into microwells and monitoring diffusion of fluorescein, a product of the enzymatic reaction of β-galactosidase and its fluorogenic substrate FDG, between droplets. View Full-Text
Keywords: floating droplet array; droplet-based microfluidics; droplet trapping; high-throughput analysis; digital detection; droplet clustering floating droplet array; droplet-based microfluidics; droplet trapping; high-throughput analysis; digital detection; droplet clustering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Labanieh, L.; Nguyen, T.N.; Zhao, W.; Kang, D.-K. Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysisand Recovery. Micromachines 2015, 6, 1469-1482.

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