Abstract: In this paper, we describe our all-titanium microelectrode array (tMEA) fabrication process and show that uncoated titanium microelectrodes are fully applicable to measuring field potentials (FPs) from neurons and cardiomyocytes. Many novel research questions require custom designed microelectrode configurations different from the few commercially available ones. As several different configurations may be needed especially in a prototyping phase, considerable time and cost savings in MEA fabrication can be achieved by omitting the additional low impedance microelectrode coating, usually made of titanium nitride (TiN) or platinum black, and have a simplified and easily processable MEA structure instead. Noise, impedance, and atomic force microscopy (AFM) characterization were performed to our uncoated titanium microelectrodes and commercial TiN coated microelectrodes and were supplemented by FP measurements from neurons and cardiomyocytes on both platforms. Despite the increased noise levels compared to commercial MEAs our tMEAs produced good FP measurements from neurons and cardiomyocytes. Thus, tMEAs offer a cost effective platform to develop custom designed electrode configurations and more complex monitoring environments.
Keywords: microelectrode array (MEA); measurement noise; impedance; stem cell; field potential measurement; titanium
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Ryynänen, T.; Kujala, V.; Ylä-Outinen, L.; Korhonen, I.; Tanskanen, J.M.; Kauppinen, P.; Aalto-Setälä, K.; Hyttinen, J.; Kerkelä, E.; Narkilahti, S.; Lekkala, J. All Titanium Microelectrode Array for Field Potential Measurements from Neurons and Cardiomyocytes—A Feasibility Study. Micromachines 2011, 2, 394-409.
Ryynänen T, Kujala V, Ylä-Outinen L, Korhonen I, Tanskanen JM, Kauppinen P, Aalto-Setälä K, Hyttinen J, Kerkelä E, Narkilahti S, Lekkala J. All Titanium Microelectrode Array for Field Potential Measurements from Neurons and Cardiomyocytes—A Feasibility Study. Micromachines. 2011; 2(4):394-409.
Ryynänen, Tomi; Kujala, Ville; Ylä-Outinen, Laura; Korhonen, Ismo; Tanskanen, Jarno M.A.; Kauppinen, Pasi; Aalto-Setälä, Katriina; Hyttinen, Jari; Kerkelä, Erja; Narkilahti, Susanna; Lekkala, Jukka. 2011. "All Titanium Microelectrode Array for Field Potential Measurements from Neurons and Cardiomyocytes—A Feasibility Study." Micromachines 2, no. 4: 394-409.