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Toxins 2017, 9(7), 222; doi:10.3390/toxins9070222

Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A

1
Laboratoire de Mycologie et Sécurité des Aliments (LMSA), Centre d’analyse et de Recherche (CAR), Campus des Sciences et Technologie, Université Saint-Joseph, Dekwaneh-Beyrouth 1104-2020, Lebanon
2
Laboratoire de Génie Chimique, CNRS, INPT, UPS, Université de Toulouse, Toulouse 31 326, France
3
Laboratory of Microbiology, Department of Natural Sciences and Earth, Faculty of Sciences I, Lebanese University, Hadath Campus, P.O Box 5 Beirut, Lebanon
*
Author to whom correspondence should be addressed.
Academic Editors: Antonio Logrieco and Sarah De Saeger
Received: 19 May 2017 / Revised: 24 June 2017 / Accepted: 9 July 2017 / Published: 14 July 2017
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Abstract

Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity for binding and metabolizing the OTA, as well as their ability to reduce the expression of the genes responsible for its production in A. carbonarius. In the first part of this study, we evaluated the capacity of Streptomyces strains for binding OTA on their surfaces after 0, 30 and 60 min of incubation with PBS solution supplemented with OTA. In the second part, we tested the ability of these strains, as well as their supernatants, to detoxify the ISP2 medium. Finally, we studied the effect of the Streptomyces cocultured with Aspergillus carbonarius on the expression of OTA biosynthesis genes. Results showed that, among the strains co-cultured with A. carbonarius, the strain G10 was able to reduce the expression of acpks, acOTApks, acOTAnrps and vea genes, thus reducing OTA from solid PDA medium to 13.50% of reduction. This strain was remarkably able to detoxify and bind OTA up to 47.07%. Strain AT8 was stronger in detoxifying OTA (52.61%), but had no significant effect on the studied gene expression. View Full-Text
Keywords: Ochratoxin A; actinobacteria; biocontrol; binding; detoxification; genes expression Ochratoxin A; actinobacteria; biocontrol; binding; detoxification; genes expression
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MDPI and ACS Style

Khoury, R.E.; Mathieu, F.; Atoui, A.; Kawtharani, H.; Khoury, A.E.; Afif, C.; Maroun, R.G.; Khoury, A.E. Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A. Toxins 2017, 9, 222.

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