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Toxins 2017, 9(5), 153; doi:10.3390/toxins9050153

Distinct Expression of Immunoglobulin-Binding Proteins in Shiga Toxin-Producing Escherichia coli Implicates High Protein Stability and a Characteristic Phenotype

Institute for Hygiene, Westfälische Wilhelms-University and University Hospital Münster, Robert Koch-Straße 41, 48149 Münster, Germany
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Academic Editor: Vernon L. Tesh
Received: 28 February 2017 / Revised: 27 April 2017 / Accepted: 27 April 2017 / Published: 29 April 2017
(This article belongs to the Section Bacterial Toxins)
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Abstract

Several immunoglobulin-binding proteins of Escherichia coli (Eib) have been isolated from both non-pathogenic and pathogenic E. coli strains. Shiga toxin (Stx)-producing E. coli (STEC) contain eibG either as a single gene or in combination with eibC, while other E. coli strains harbour single or multiple eib genes. The Eib proteins bind human immunoglobulins in a non-immune manner and contribute to bacterial chain-like adherence to human epithelial cells. In this study, the EibG expression in several STEC strains was analysed under different environmental conditions. STEC produced high levels of EibG in complex media and lower levels in low-grade and minimal media under static growth conditions. This characteristic was independent on the Eib subtypes. Microscopically, EibG-expressing STEC exhibited chain formation and aggregation in all employed media, while aggregates were only visible after growth in complex medium. Once expressed, EibG proteins demonstrate high stability during prolonged incubation. Our findings indicate that the regulation of the expression of Eib proteins is highly complex, although the protein levels vary among STEC strains. However, positive upregulation conditions generally result in distinct phenotypes of the isolates. View Full-Text
Keywords: regulation; expression; immunoglobulin-binding protein G; Shiga toxin-producing Escherichia coli regulation; expression; immunoglobulin-binding protein G; Shiga toxin-producing Escherichia coli
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MDPI and ACS Style

Rubin, D.; Zhang, W.; Karch, H.; Kuczius, T. Distinct Expression of Immunoglobulin-Binding Proteins in Shiga Toxin-Producing Escherichia coli Implicates High Protein Stability and a Characteristic Phenotype. Toxins 2017, 9, 153.

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