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Toxins 2017, 9(10), 318; doi:10.3390/toxins9100318

Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk

1,2,3
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1,2,3,4,* , 1,2,3,4,* , 1,2,4
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1
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Laboratory of Quality & Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
4
Quality Inspection & Test Center for Oilseed Products, Ministry of Agriculture, Wuhan 430062, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 12 September 2017 / Revised: 5 October 2017 / Accepted: 6 October 2017 / Published: 13 October 2017
(This article belongs to the Collection Aflatoxins)
View Full-Text   |   Download PDF [3667 KB, uploaded 13 October 2017]   |  

Abstract

A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants. View Full-Text
Keywords: aflatoxin M1; palladium nanoparticles; aptasensor; fluorescence resonance energy transfer aflatoxin M1; palladium nanoparticles; aptasensor; fluorescence resonance energy transfer
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Li, H.; Yang, D.; Li, P.; Zhang, Q.; Zhang, W.; Ding, X.; Mao, J.; Wu, J. Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk. Toxins 2017, 9, 318.

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