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Toxins 2017, 9(10), 305; doi:10.3390/toxins9100305

Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay

Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Bari 70126, Italy
Barilla Advanced Laboratory Research, Parma 43122, Italy
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 14 September 2017 / Revised: 21 September 2017 / Accepted: 22 September 2017 / Published: 26 September 2017
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
View Full-Text   |   Download PDF [809 KB, uploaded 23 October 2017]   |  


A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard deviations lower than 6%. Limits of detection (LOD) of the optimized FPIA was 0.6 μg/kg for rye and rye crispbread, respectively. Good correlations (r > 0.977) were observed between OTA contents in contaminated samples obtained by FPIA and high-performance liquid chromatography (HPLC) with immunoaffinity cleanup used as reference method. Furthermore, single laboratory validation and small-scale collaborative trials were carried out for the determination of OTA in rye according to Regulation 519/2014/EU laying down procedures for the validation of screening methods. The precision profile of the method, cut-off level and rate of false suspect results confirm the satisfactory analytical performances of assay as a screening method. These findings show that the optimized FPIA is suitable for high-throughput screening, and permits reliable quantitative determination of OTA in rye and rye crispbread at levels that fall below the EU regulatory limits. View Full-Text
Keywords: fluorescence polarization immunoassay; ochratoxin A; rye; rye-based products; validation study fluorescence polarization immunoassay; ochratoxin A; rye; rye-based products; validation study

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MDPI and ACS Style

Lippolis, V.; Porricelli, A.C.R.; Cortese, M.; Suman, M.; Zanardi, S.; Pascale, M. Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay. Toxins 2017, 9, 305.

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