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Toxins 2017, 9(10), 305; https://doi.org/10.3390/toxins9100305

Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay

1
Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Bari 70126, Italy
2
Barilla Advanced Laboratory Research, Parma 43122, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 14 September 2017 / Revised: 21 September 2017 / Accepted: 22 September 2017 / Published: 26 September 2017
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
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Abstract

A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard deviations lower than 6%. Limits of detection (LOD) of the optimized FPIA was 0.6 μg/kg for rye and rye crispbread, respectively. Good correlations (r > 0.977) were observed between OTA contents in contaminated samples obtained by FPIA and high-performance liquid chromatography (HPLC) with immunoaffinity cleanup used as reference method. Furthermore, single laboratory validation and small-scale collaborative trials were carried out for the determination of OTA in rye according to Regulation 519/2014/EU laying down procedures for the validation of screening methods. The precision profile of the method, cut-off level and rate of false suspect results confirm the satisfactory analytical performances of assay as a screening method. These findings show that the optimized FPIA is suitable for high-throughput screening, and permits reliable quantitative determination of OTA in rye and rye crispbread at levels that fall below the EU regulatory limits. View Full-Text
Keywords: fluorescence polarization immunoassay; ochratoxin A; rye; rye-based products; validation study fluorescence polarization immunoassay; ochratoxin A; rye; rye-based products; validation study
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Lippolis, V.; Porricelli, A.C.R.; Cortese, M.; Suman, M.; Zanardi, S.; Pascale, M. Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay. Toxins 2017, 9, 305.

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