Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins
Abstract
:1. Introduction
2. Results
2.1. Growth Performance
2.2. Serum Antioxidant Indices
2.3. Liver Antioxidant Indices
2.4. Aflatoxin Residues in Liver
3. Discussion
3.1. Growth Performance
3.2. Serum and Liver Antioxidant Indices
3.3. Aflatoxin Residues in Liver
4. Conclusions
5. Materials and Methods
5.1. Ducks, Diets, and Management
5.2. Analysis of Dietary Mycotoxin
5.3. Sampling of Antioxidant Indices and Measurements
5.4. Analysis of Aflatoxin Residues in Liver
5.5. Statistical Analyses
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Item | Initial BW, g | Final BW, g | ADG, g/bird d | ADFI, g/bird d | F:G, g/g |
---|---|---|---|---|---|
C0 | 759.75 ± 7.13 | 3159.73 ± 19.62 | 109.09 ± 0.78 | 235.28 ± 1.79 | 2.17 ± 0.02 a |
M0 | 758.15 ± 6.02 | 3120.93 ± 8.43 | 107.40 ± 0.53 | 234.28 ± 0.61 | 2.18 ± 0.01 a |
M500 | 754.92 ± 3.61 | 3193.96 ± 22.20 | 110.86 ± 1.05 | 237.23 ± 3.08 | 2.14 ± 0.02 a |
M1000 | 758.47 ± 5.26 | 3195.05 ± 11.78 | 110.75 ± 0.46 | 230.38 ± 2.21 | 2.08 ± 0.02 b |
M2000 | 759.60 ± 7.01 | 3186.97 ± 23.95 | 110.33 ± 1.31 | 237.32 ± 2.44 | 2.17 ± 0.03 a |
p Value | 0.967 | 0.067 | 0.075 | 0.332 | 0.014 |
Item | T-SOD, U/mL | GSH-Px, U/mL | MDA, nmol/mL |
---|---|---|---|
C0 | 112.41 ± 4.32 a,b | 1718.89 ± 141.66 | 2.55 ± 0.16 |
M0 | 107.36 ± 2.70 a | 1508.71 ± 184.24 | 2.83 ± 0.32 |
M500 | 121.50 ± 2.13 b | 1601.39 ± 81.31 | 2.62 ± 0.16 |
M1000 | 121.34 ± 4.12 b | 1818.50 ± 96.85 | 2.58 ± 0.28 |
M2000 | 120.30 ± 3.99 b | 1818.50 ± 264.37 | 2.45 ± 0.20 |
p Value | 0.029 | 0.581 | 0.810 |
Item | T-SOD, U/mgprot | GSH-Px, U/mgprot | MDA, nmol/mgprot |
---|---|---|---|
C0 | 111.10 ± 5.73 c | 116.45 ± 6.12 a | 0.55 ± 0.05 |
M0 | 85.21 ± 3.44 a | 87.11 ± 5.28 b | 0.90 ± 0.29 |
M500 | 104.83 ± 3.50 b,c | 109.96 ± 3.31 a | 0.79 ± 0.08 |
M1000 | 98.37 ± 3.00 b | 106.58 ± 3.82 a | 0.56 ± 0.05 |
M2000 | 105.80 ± 3.92 b,c | 104.44 ± 5.86 a | 0.64 ± 0.06 |
p-value | 0.001 | 0.007 | 0.398 |
Item | AFB1 | AFB2 | AFG1 | AFG2 | AFM1 |
---|---|---|---|---|---|
C0 | ND * | ND | ND | ND | ND |
M0 | 0.12 ± 0.01 a | ND | ND | ND | 0.10 ± 0.02 a |
M500 | 0.07 ± 0.01 b | ND | ND | ND | 0.06 ± 0.00 b |
M1000 | 0.06 ± 0.01 c | ND | ND | ND | 0.06 ± 0.01 b |
M2000 | 0.05 ± 0.01 d | ND | ND | ND | 0.05 ± 0.01 b |
Ingredients | Percentage (%) | Nutrition Component | Content |
---|---|---|---|
Maize | 60.00 | Crude protein, % | 16.50 |
Soybean meal | 13.64 | Metabolizable energy, MJ/kg | 12.39 |
Wheat | 4.75 | Calcium, % | 1.00 |
Flour | 3.50 | Total phosphorus, % | 0.67 |
Peanut meal | 4.20 | Available phosphorus, % | 0.42 |
Zein meal | 3.50 | Methionine, % | 0.46 |
Calcium hydrophosphate | 1.36 | Methionine + Cystine, % | 0.76 |
Limestone | 1.36 | Lysine, % | 0.96 |
Salt | 0.32 | ||
Soybean oil | 1.77 | ||
Rice bran | 2.30 | ||
Rice bran meal | 2.00 | ||
Premix 1 | 1.30 | ||
Total | 100.00 |
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Zhang, L.; Ma, Q.; Ma, S.; Zhang, J.; Jia, R.; Ji, C.; Zhao, L. Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins. Toxins 2017, 9, 1. https://doi.org/10.3390/toxins9010001
Zhang L, Ma Q, Ma S, Zhang J, Jia R, Ji C, Zhao L. Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins. Toxins. 2017; 9(1):1. https://doi.org/10.3390/toxins9010001
Chicago/Turabian StyleZhang, Liyuan, Qiugang Ma, Shanshan Ma, Jianyun Zhang, Ru Jia, Cheng Ji, and Lihong Zhao. 2017. "Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins" Toxins 9, no. 1: 1. https://doi.org/10.3390/toxins9010001