Next Article in Journal
Heterologous Expression of Toxins from Bacterial Toxin-Antitoxin Systems in Eukaryotic Cells: Strategies and Applications
Next Article in Special Issue
Use of Cold Atmospheric Plasma to Detoxify Hazelnuts from Aflatoxins
Previous Article in Journal
Lolitrem B and Indole Diterpene Alkaloids Produced by Endophytic Fungi of the Genus Epichloë and Their Toxic Effects in Livestock
Previous Article in Special Issue
The Master Transcription Factor mtfA Governs Aflatoxin Production, Morphological Development and Pathogenicity in the Fungus Aspergillus flavus
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Toxins 2016, 8(2), 46; doi:10.3390/toxins8020046

Functional Genomic Analysis of Aspergillus flavus Interacting with Resistant and Susceptible Peanut

1
Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China
3
Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Academic Editor: Shohei Sakuda
Received: 30 December 2015 / Revised: 30 January 2016 / Accepted: 5 February 2016 / Published: 15 February 2016
(This article belongs to the Collection Aflatoxins)
View Full-Text   |   Download PDF [1299 KB, uploaded 15 February 2016]   |  

Abstract

In the Aspergillus flavus (A. flavus)–peanut pathosystem, development and metabolism of the fungus directly influence aflatoxin contamination. To comprehensively understand the molecular mechanism of A. flavus interaction with peanut, RNA-seq was used for global transcriptome profiling of A. flavus during interaction with resistant and susceptible peanut genotypes. In total, 67.46 Gb of high-quality bases were generated for A. flavus-resistant (af_R) and -susceptible peanut (af_S) at one (T1), three (T2) and seven (T3) days post-inoculation. The uniquely mapped reads to A. flavus reference genome in the libraries of af_R and af_S at T2 and T3 were subjected to further analysis, with more than 72% of all obtained genes expressed in the eight libraries. Comparison of expression levels both af_R vs. af_S and T2 vs. T3 uncovered 1926 differentially expressed genes (DEGs). DEGs associated with mycelial growth, conidial development and aflatoxin biosynthesis were up-regulated in af_S compared with af_R, implying that A. flavus mycelia more easily penetrate and produce much more aflatoxin in susceptible than in resistant peanut. Our results serve as a foundation for understanding the molecular mechanisms of aflatoxin production differences between A. flavus-R and -S peanut, and offer new clues to manage aflatoxin contamination in crops. View Full-Text
Keywords: Aspergillus flavus; peanut; interaction; aflatoxin biosynthesis; RNA-seq Aspergillus flavus; peanut; interaction; aflatoxin biosynthesis; RNA-seq
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, H.; Lei, Y.; Yan, L.; Wan, L.; Ren, X.; Chen, S.; Dai, X.; Guo, W.; Jiang, H.; Liao, B. Functional Genomic Analysis of Aspergillus flavus Interacting with Resistant and Susceptible Peanut. Toxins 2016, 8, 46.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top