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Toxins 2016, 8(12), 372; doi:10.3390/toxins8120372

Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques

1
Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland
2
Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic
3
Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovac Republic
4
Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic
5
Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Syed A. Ali
Received: 26 October 2016 / Revised: 22 November 2016 / Accepted: 6 December 2016 / Published: 13 December 2016
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [3303 KB, uploaded 13 December 2016]   |  

Abstract

Snake venom is a complex mixture of proteins and peptides which in the Viperidae is mainly hemotoxic. The diversity of these components causes the venom to be an extremely interesting object of study. Discovered components can be used in search for new pharmaceuticals used primarily in the treatment of diseases of the cardiovascular system. In order to determine the protein composition of the southern copperhead venom, we have used high resolution two dimensional electrophoresis and MALDI ToF/ToF MS-based identification. We have identified 10 groups of proteins present in the venom, of which phospholipase A2 and metalloprotease and serine proteases constitute the largest groups. For the first time presence of 5′-nucleotidase in venom was found in this group of snakes. Three peptides present in the venom were also identified. Two of them as bradykinin-potentiating agents and one as an inhibitor. View Full-Text
Keywords: Agkistrodon contortrix contortrix; southern copperhead; venom; proteomics Agkistrodon contortrix contortrix; southern copperhead; venom; proteomics
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MDPI and ACS Style

Bocian, A.; Urbanik, M.; Hus, K.; Łyskowski, A.; Petrilla, V.; Andrejčáková, Z.; Petrillová, M.; Legáth, J. Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques. Toxins 2016, 8, 372.

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