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Toxins 2016, 8(11), 313; doi:10.3390/toxins8110313

Effect of Various Compounds Blocking the Colony Pigmentation on the Aflatoxin B1 Production by Aspergillus flavus

All-Russian Research Institute of Phytopathology, Bolshie Vyazemy, Moscow 143050, Russia
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Academic Editors: Kris Audenaert, Sarah De Saeger and Siska Croubels
Received: 15 September 2016 / Revised: 17 October 2016 / Accepted: 24 October 2016 / Published: 28 October 2016
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Abstract

Aflatoxins and melanins are the products of a polyketide biosynthesis. In this study, the search of potential inhibitors of the aflatoxin B1 (AFB1) biosynthesis was performed among compounds blocking the pigmentation in fungi. Four compounds—three natural (thymol, 3-hydroxybenzaldehyde, compactin) and one synthetic (fluconazole)—were examined for their ability to block the pigmentation and AFB1 production in Aspergillus flavus. All compounds inhibited the mycelium pigmentation of a fungus growing on solid medium. At the same time, thymol, fluconazole, and 3-hydroxybenzaldehyde stimulated AFB1 accumulation in culture broth of A. flavus under submerged fermentation, whereas the addition of 2.5 μg/mL of compactin resulted in a 50× reduction in AFB1 production. Moreover, compactin also suppressed the sporulation of A. flavus on solid medium. In vivo treatment of corn and wheat grain with compactin (50 μg/g of grain) reduced the level of AFB1 accumulation 14 and 15 times, respectively. Further prospects of the compactin study as potential AFB1 inhibitor are discussed. View Full-Text
Keywords: aflatoxin B1; fungal melanins; polyketide biosynthesis; compactin; thymol; fluconazole; 3-hydroxybenzaldehyde; Aspergillus flavus aflatoxin B1; fungal melanins; polyketide biosynthesis; compactin; thymol; fluconazole; 3-hydroxybenzaldehyde; Aspergillus flavus
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Dzhavakhiya, V.G.; Voinova, T.M.; Popletaeva, S.B.; Statsyuk, N.V.; Limantseva, L.A.; Shcherbakova, L.A. Effect of Various Compounds Blocking the Colony Pigmentation on the Aflatoxin B1 Production by Aspergillus flavus. Toxins 2016, 8, 313.

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