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Toxins 2016, 8(10), 296; doi:10.3390/toxins8100296

Crystal Structure of Ribosome-Inactivating Protein Ricin A Chain in Complex with the C-Terminal Peptide of the Ribosomal Stalk Protein P2

Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editors: Julien Barbier and Daniel Gillet
Received: 24 August 2016 / Revised: 27 September 2016 / Accepted: 30 September 2016 / Published: 13 October 2016
(This article belongs to the Special Issue Ribosome Inactivating Toxins)
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Abstract

Ricin is a type 2 ribosome-inactivating protein (RIP), containing a catalytic A chain and a lectin-like B chain. It inhibits protein synthesis by depurinating the N-glycosidic bond at α-sarcin/ricin loop (SRL) of the 28S rRNA, which thereby prevents the binding of elongation factors to the GTPase activation center of the ribosome. Here, we present the 1.6 Å crystal structure of Ricin A chain (RTA) complexed to the C-terminal peptide of the ribosomal stalk protein P2, which plays a crucial role in specific recognition of elongation factors and recruitment of eukaryote-specific RIPs to the ribosomes. Our structure reveals that the C-terminal GFGLFD motif of P2 peptide is inserted into a hydrophobic pocket of RTA, while the interaction assays demonstrate the structurally untraced SDDDM motif of P2 peptide contributes to the interaction with RTA. This interaction mode of RTA and P protein is in contrast to that with trichosanthin (TCS), Shiga-toxin (Stx) and the active form of maize RIP (MOD), implying the flexibility of the P2 peptide-RIP interaction, for the latter to gain access to ribosome. View Full-Text
Keywords: Ricin; ribosome-inactivating protein; ribosomal P stalk protein; ribosome Ricin; ribosome-inactivating protein; ribosomal P stalk protein; ribosome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shi, W.-W.; Tang, Y.-S.; Sze, S.-Y.; Zhu, Z.-N.; Wong, K.-B.; Shaw, P.-C. Crystal Structure of Ribosome-Inactivating Protein Ricin A Chain in Complex with the C-Terminal Peptide of the Ribosomal Stalk Protein P2. Toxins 2016, 8, 296.

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