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Toxins 2015, 7(9), 3554-3571; doi:10.3390/toxins7093554

Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids

1
Laboratorio de Toxicología, Departament de Medicina Preventiva I Salut Pública, Ciències de l’Alimentació, Toxicologia I Medicina Legal Facultat de Farmàcia, Universitat de València, València 46010, Spain
2
Department of Chemistry, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, Rome 00185, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 29 July 2015 / Accepted: 31 August 2015 / Published: 9 September 2015
(This article belongs to the Section Mycotoxins)
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Abstract

A novel method for the simultaneous determination of enniatins A, A1, B and B1 and beauvericin, both in human urine and plasma samples, was developed and validated. The method consisted of a simple and easy pretreatment, specific for each matrix, followed by solid phase extraction (SPE) and detection by high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. The optimized SPE method was performed on graphitized carbon black cartridges after suitable dilution of the extracts, which allowed high mycotoxin absolute recoveries (76%–103%) and the removal of the major interferences from the matrix. The method was extensively evaluated for plasma and urine samples separately, providing satisfactory results in terms of linearity (R2 of 0.991–0.999), process efficiency (>81%), trueness (recoveries between 85% and 120%), intra-day precision (relative standard deviation, RSD < 18%), inter-day precision (RSD < 21%) and method quantification limits (ranging between 20 ng·L1 and 40 ng·L1 in plasma and between 5 ng·L1 and 20 ng·L1 in urine). Finally, the highly sensitive validated method was applied to some urine and plasma samples from different donors. View Full-Text
Keywords: mycotoxins; enniatins; beauvericin; urine; plasma; liquid chromatography-tandem mass spectrometry mycotoxins; enniatins; beauvericin; urine; plasma; liquid chromatography-tandem mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Serrano, A.B.; Capriotti, A.L.; Cavaliere, C.; Piovesana, S.; Samperi, R.; Ventura, S.; Laganà, A. Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids. Toxins 2015, 7, 3554-3571.

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