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Toxins 2015, 7(6), 2145-2158; doi:10.3390/toxins7062145

Prevention of Aflatoxin B1-Induced DNA Breaks by β-D-Glucan

Conservation Medicine Laboratory, Superior School of Medicine, IPN. "Unidad Casco de Santo Tomas". Plan de San Luis y Díaz Mirón. México, DF 11340, Mexico
Genetics Laboratory, National School of Biological Sciences, IPN. "Unidad A. López Mateos". Av. Wilfrido Massieu. Zacatenco, México, DF 07738, Mexico
Institute of Health Sciences, Autonomous University of Hidalgo State, Ex-Hacienda de la Concepción, Tilcuautla, Hidalgo 42160, Mexico
Organic Chemistry Department, National School of Biological Sciences, IPN. "Unidad Casco de Santo Tomas". Carpio y Plan de Ayala. México, DF 11340, Mexico
Preclinical Toxicology Laboratory, National School of Biological Sciences, IPN. "Unidad A. López Mateos". Av. Wilfrido Massieu. Zacatenco, México, DF 07738, Mexico
Author to whom correspondence should be addressed.
Academic Editor: Richard A. Manderville
Received: 16 March 2015 / Revised: 19 May 2015 / Accepted: 28 May 2015 / Published: 11 June 2015
(This article belongs to the Special Issue Mycotoxins and Human Diseases 2015)
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Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B1 (AFB1) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-D-glucan (Glu) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB1 and β-D-glucan. View Full-Text
Keywords: aflatoxin B1; glucan; mouse hepatocytes; antigenotoxicity aflatoxin B1; glucan; mouse hepatocytes; antigenotoxicity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Madrigal-Bujaidar, E.; Morales-González, J.A.; Sánchez-Gutiérrez, M.; Izquierdo-Vega, J.A.; Reyes-Arellano, A.; Álvarez-González, I.; Pérez-Pasten, R.; Madrigal-Santillán, E. Prevention of Aflatoxin B1-Induced DNA Breaks by β-D-Glucan. Toxins 2015, 7, 2145-2158.

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