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Toxins 2015, 7(5), 1765-1778; doi:10.3390/toxins7051765

Recommended Mass Spectrometry-Based Strategies to Identify Botulinum Neurotoxin-Containing Samples

Centers for Disease Control and Prevention, 4770 Buford Hwy NE, Atlanta, GA 30341, USA
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Academic Editors: Andreas Rummel and Brigitte G. Dorner
Received: 17 April 2015 / Revised: 4 May 2015 / Accepted: 11 May 2015 / Published: 19 May 2015
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Abstract

Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are proteins secreted by some species of clostridia and are known to cause paralysis by interfering with nerve impulse transmission. Although the human lethal dose of BoNT is not accurately known, it is estimated to be between 0.1 μg to 70 μg, so it is important to enable detection of small amounts of these toxins. Our laboratory previously reported on the development of Endopep-MS, a mass-spectrometric‑based endopeptidase method to detect, differentiate, and quantify BoNT immunoaffinity purified from complex matrices. In this work, we describe the application of Endopep-MS for the analysis of thirteen blinded samples supplied as part of the EQuATox proficiency test. This method successfully identified the presence or absence of BoNT in all thirteen samples and was able to successfully differentiate the serotype of BoNT present in the samples, which included matrices such as buffer, milk, meat extract, and serum. Furthermore, the method yielded quantitative results which had z-scores in the range of −3 to +3 for quantification of BoNT/A containing samples. These results indicate that Endopep-MS is an excellent technique for detection, differentiation, and quantification of BoNT in complex matrices. View Full-Text
Keywords: botulinum neurotoxin; botulism; mass spectrometry botulinum neurotoxin; botulism; mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kalb, S.R.; Baudys, J.; Wang, D.; Barr, J.R. Recommended Mass Spectrometry-Based Strategies to Identify Botulinum Neurotoxin-Containing Samples. Toxins 2015, 7, 1765-1778.

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