Next Article in Journal
Conotoxins That Could Provide Analgesia through Voltage Gated Sodium Channel Inhibition
Next Article in Special Issue
Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins
Previous Article in Journal
Prophage-Encoded Staphylococcal Enterotoxin A: Regulation of Production in Staphylococcus aureus Strains Representing Different Sea Regions
Previous Article in Special Issue
A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples
Article Menu

Export Article

Open AccessArticle
Toxins 2015, 7(12), 5377-5385; doi:10.3390/toxins7124883

Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

1
Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
2
Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China
3
Grain Safety, Academy of State Administration of Grain, Beijing 100037, China
*
Author to whom correspondence should be addressed.
Academic Editors: Michelangelo Pascale and Maria C. DeRosa
Received: 16 October 2015 / Revised: 30 November 2015 / Accepted: 1 December 2015 / Published: 10 December 2015
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
View Full-Text   |   Download PDF [3621 KB, uploaded 10 December 2015]   |  

Abstract

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection. View Full-Text
Keywords: aptamer; Ochratoxin A (OTA); gold nanoparticles; cationic polymer; colorimetric assay aptamer; Ochratoxin A (OTA); gold nanoparticles; cationic polymer; colorimetric assay
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Luan, Y.; Chen, J.; Li, C.; Xie, G.; Fu, H.; Ma, Z.; Lu, A. Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Toxins 2015, 7, 5377-5385.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top