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Toxins 2015, 7(1), 97-109; doi:10.3390/toxins7010097

Nephroprotective Role of Resveratrol and Ursolic Acid in Aristolochic Acid Intoxicated Zebrafish

1
Department of Chemistry, Tamkang University, No. 151 Ying-chuan Road, Tamsui, New Taipei City 25137, Taiwan
2
Department of Nephrology, Chang Gung Memorial Hospital, Keelung 20491, Taiwan
3
Department of Mathematics, Tamkang University, Tamsui, New Taipei City 25137, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: John P. Berry
Received: 6 September 2014 / Accepted: 5 January 2015 / Published: 13 January 2015
(This article belongs to the Section Marine and Freshwater Toxins)
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Abstract

The nephrotoxicity of aristolochic acid (AA) is well known, but information regarding the attenuation of AA-induced toxicity is limited. The aim of the present study was to study the nephroprotective effects of resveratrol (Resv) and ursolic acid (UA) in a zebrafish model. We used two transgenic lines, Tg(wt1b:EGFP) and Tg(gata1:DsRed), to evaluate the nephroprotective effects of Resv and UA by recording subtle changes in the kidney and red blood cell circulation. Our results demonstrated that both Resv and UA treatment can attenuate AA-induced kidney malformations and improve blood circulation. Glomerular filtration rate assays revealed that both Resv and UA treatment can restore renal function (100% for Mock; 56.1% ± 17.3% for AA-treated; 80.2% ± 11.3% for Resv+AA; and 83.1% ± 8.1% for UA+AA, n = 15). Furthermore, real-time RT-PCR experiments showed that pre-treatment with either Resv or UA suppresses expression of pro-inflammatory genes. In conclusion, our findings reveal that AA-induced nephrotoxicities can be attenuated by pre-treatment with either Resv or UA. Therefore, we believe that zebrafish represent an efficient model for screening AA-protective natural compounds. View Full-Text
Keywords: aristolochic acid; kidney; nephrotoxicity; resveratrol; ursolic acid; zebrafish aristolochic acid; kidney; nephrotoxicity; resveratrol; ursolic acid; zebrafish
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ding, Y.-J.; Sun, C.-Y.; Wen, C.-C.; Chen, Y.-H. Nephroprotective Role of Resveratrol and Ursolic Acid in Aristolochic Acid Intoxicated Zebrafish. Toxins 2015, 7, 97-109.

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