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Toxins 2014, 6(9), 2857-2871; doi:10.3390/toxins6092857

Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines

1
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary
2
Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged H-6720, Hungary
3
Department of Food and Environmental Sciences, Viikinkaari 9, University of Helsinki, Agnes Sjöbergin katu 2, Helsinki FI-00014, Finland
*
Author to whom correspondence should be addressed.
Received: 28 May 2014 / Revised: 28 August 2014 / Accepted: 1 September 2014 / Published: 23 September 2014
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Abstract

Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1–2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration. View Full-Text
Keywords: ophiobolin A; boar sperm assay; Bipolaris oryzae; cell toxicity; mitochondrial dissipation ophiobolin A; boar sperm assay; Bipolaris oryzae; cell toxicity; mitochondrial dissipation
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MDPI and ACS Style

Bencsik, O.; Papp, T.; Berta, M.; Zana, A.; Forgó, P.; Dombi, G.; Andersson, M.A.; Salkinoja-Salonen, M.; Vágvölgyi, C.; Szekeres, A. Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines. Toxins 2014, 6, 2857-2871.

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