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Toxins 2014, 6(9), 2840-2856; https://doi.org/10.3390/toxins6092840

Oxidized Lipids and Lysophosphatidylcholine Induce the Chemotaxis, Up-Regulate the Expression of CCR9 and CXCR4 and Abrogate the Release of IL-6 in Human Monocytes

Department of Physiology, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo 0317, Norway
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Received: 24 July 2014 / Revised: 8 September 2014 / Accepted: 15 September 2014 / Published: 23 September 2014
(This article belongs to the Collection Toxicity and Therapeutic Interventions in the Immune System)
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Abstract

Lipids through regulation of chronic inflammation play key roles in the development of various diseases. Here, we report that a mixed population of human primary monocytes migrated towards LPC, as well as oxidized linoleic acid isoforms 9-S-HODE, 9-R-HODE and 13-R-HODE. Incubation with 9-R-HODE, 13-R-HODE and LPC resulted in increased expression of CXCR4, the receptor for SDF-1α/CXCL12, correlated with increased monocyte migration towards SDF-1α/CXCL12. Further, we report increased expression of CCR9, the receptor for TECK/CCL25, after stimulation with these lipids. Upon examining the migratory response towards TECK/CCL25, it was observed that an increase in CCR9 expression upon pre-treatment with 9-S-HODE, 9-R-HODE, 13-R-HODE and LPC resulted in increased migration of monocytes expressing CCR9. Only LPC but not any other lipid examined increased the influx of intracellular Ca2+ in monocytes. Finally, 9-S-HODE, 9-R-HODE, 13-R-HODE, or LPC inhibited the release of IL-6 from monocytes suggesting that these lipids may play important role in controlling inflammatory responses. View Full-Text
Keywords: CCR9; CXCR4; HODE; LPC; monocytes; SDF-1α; TECK CCR9; CXCR4; HODE; LPC; monocytes; SDF-1α; TECK
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Rolin, J.; Vego, H.; Maghazachi, A.A. Oxidized Lipids and Lysophosphatidylcholine Induce the Chemotaxis, Up-Regulate the Expression of CCR9 and CXCR4 and Abrogate the Release of IL-6 in Human Monocytes. Toxins 2014, 6, 2840-2856.

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