Next Article in Journal
Determination of Urinary Biomarkers for Assessment of Short-Term Human Exposure to Aflatoxins in São Paulo, Brazil
Previous Article in Journal
Impact of Environmental Factors on the Regulation of Cyanotoxin Production
Article Menu

Export Article

Open AccessArticle
Toxins 2014, 6(7), 1979-1995; doi:10.3390/toxins6071979

Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus

1
Monash Venom Group, Department of Pharmacology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria 3168, Australia
2
Venom Science Pty Ltd, Tanunda, South Australia 5352, Australia
3
SA Pathology, IMVS Veterinary Services, Gilles Plains, South Australia 5086, Australia
4
Adelaide Zoo, Adelaide, South Australia 5000, Australia
5
Venom Supplies, Tanunda, South Australia, South Australia 5352, Australia
*
Author to whom correspondence should be addressed.
Received: 5 May 2014 / Revised: 11 June 2014 / Accepted: 16 June 2014 / Published: 2 July 2014
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [900 KB, uploaded 16 July 2014]   |  

Abstract

Taipans are highly venomous Australo-Papuan elapids. A new species of taipan, the Western Desert Taipan (Oxyuranus temporalis), has been discovered with two specimens housed in captivity at the Adelaide Zoo. This study is the first investigation of O. temporalis venom and seeks to characterise and compare the neurotoxicity, lethality and biochemical properties of O. temporalis venom with other taipan venoms. Analysis of O. temporalis venom using size-exclusion and reverse-phase HPLC indicated a markedly simplified “profile” compared to other taipan venoms. SDS-PAGE and agarose gel electrophoresis analysis also indicated a relatively simple composition. Murine LD50 studies showed that O. temporalis venom is less lethal than O. microlepidotus venom. Venoms were tested in vitro, using the chick biventer cervicis nerve-muscle preparation. Based on t90 values, O. temporalis venom is highly neurotoxic abolishing indirect twitches far more rapidly than other taipan venoms. O. temporalis venom also abolished responses to exogenous acetylcholine and carbachol, indicating the presence of postsynaptic neurotoxins. Prior administration of CSL Taipan antivenom (CSL Limited) neutralised the inhibitory effects of all taipan venoms. The results of this study suggest that the venom of the O. temporalis is highly neurotoxic in vitro and may contain procoagulant toxins, making this snake potentially dangerous to humans. View Full-Text
Keywords: Oxyuranus temporalis; taipan; antivenom; neurotoxicity; snake; venom Oxyuranus temporalis; taipan; antivenom; neurotoxicity; snake; venom
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Barber, C.M.; Madaras, F.; Turnbull, R.K.; Morley, T.; Dunstan, N.; Allen, L.; Kuchel, T.; Mirtschin, P.; Hodgson, W.C. Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus. Toxins 2014, 6, 1979-1995.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top