Toxins 2014, 6(3), 988-1001; doi:10.3390/toxins6030988
Article

The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons

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Received: 27 January 2014; in revised form: 10 February 2014 / Accepted: 17 February 2014 / Published: 5 March 2014
(This article belongs to the Section Animal Venoms)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Selenocosmia jiafu is a medium-sized theraphosid spider and an attractive source of venom, because it can be bred in captivity and it produces large amounts of venom. We performed reversed-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses and showed that S. jiafu venom contains hundreds of peptides with a predominant mass of 3000–4500 Da. Patch clamp analyses indicated that the venom could inhibit voltage-gated Na+, K+ and Ca2+ channels in rat dorsal root ganglion (DRG) neurons. The venom exhibited inhibitory effects on tetrodotoxin-resistant (TTX-R) Na+ currents and T-type Ca2+ currents, suggesting the presence of antagonists to both channel types and providing a valuable tool for the investigation of these channels and for drug development. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 84.24 μg/g of body weight in the cockroach. However, no visible symptoms or behavioral changes were detected after intraperitoneal injection of the venom into mice even at doses up to 10 mg/kg body weight. Our results provide a basis for further case-by-case investigations of peptide toxins from this venom.
Keywords: Selenocosmia jiafu; spider venom; RP-HPLC; MALDI-TOF-MS; patch clamp analysis; voltage-gated ion channel; DRG neuron
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MDPI and ACS Style

Hu, Z.; Zhou, X.; Chen, J.; Tang, C.; Xiao, Z.; Ying, D.; Liu, Z.; Liang, S. The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons. Toxins 2014, 6, 988-1001.

AMA Style

Hu Z, Zhou X, Chen J, Tang C, Xiao Z, Ying D, Liu Z, Liang S. The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons. Toxins. 2014; 6(3):988-1001.

Chicago/Turabian Style

Hu, Zhaotun; Zhou, Xi; Chen, Jia; Tang, Cheng; Xiao, Zhen; Ying, Dazhong; Liu, Zhonghua; Liang, Songping. 2014. "The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons." Toxins 6, no. 3: 988-1001.

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