Bt Toxin Modification for Enhanced Efficacy
AbstractInsect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for pest suppression. Here we review the different strategies that have been employed to enhance toxicity against specific target species including those that have evolved resistance to Bt, or to modify the host range of Bt crystal (Cry) and cytolytic (Cyt) toxins. These strategies include toxin truncation, modification of protease cleavage sites, domain swapping, site-directed mutagenesis, peptide addition, and phage display screens for mutated toxins with enhanced activity. Toxin optimization provides a useful approach to extend the utility of these proteins for suppression of pests that exhibit low susceptibility to native Bt toxins, and to overcome field resistance. View Full-Text
Share & Cite This Article
Deist, B.R.; Rausch, M.A.; Fernandez-Luna, M.T.; Adang, M.J.; Bonning, B.C. Bt Toxin Modification for Enhanced Efficacy. Toxins 2014, 6, 3005-3027.
Deist BR, Rausch MA, Fernandez-Luna MT, Adang MJ, Bonning BC. Bt Toxin Modification for Enhanced Efficacy. Toxins. 2014; 6(10):3005-3027.Chicago/Turabian Style
Deist, Benjamin R.; Rausch, Michael A.; Fernandez-Luna, Maria T.; Adang, Michael J.; Bonning, Bryony C. 2014. "Bt Toxin Modification for Enhanced Efficacy." Toxins 6, no. 10: 3005-3027.