Toxins 2013, 5(3), 590-604; doi:10.3390/toxins5030590
Article

Detection of Type A Trichothecene Di-Glucosides Produced in Corn by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry

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Received: 26 February 2013; in revised form: 19 March 2013 / Accepted: 19 March 2013 / Published: 22 March 2013
(This article belongs to the Special Issue Mycotoxins in Food and Feed)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The existence of di-glucosylated derivative of T-2 toxin in plant (corn powder) was confirmed for the first time in addition to that of HT-2 toxin. These masked mycotoxins (mycotoxin glucosides) were identified as T-2 toxin-di-glucoside (T2GlcGlc) and HT-2 toxin-di-glucoside (HT2GlcGlc) based on accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometric (LC-Orbitrap MS) analysis. Although the absolute structure of T2GlcGlc was not clarified, two glucose molecules were suggested to be conjugated at 3-OH position in tandem when considering the structure of T-2 toxin. On the other hand, the specification of the structure seems to be more complicated in the case of HT2GlcGlc, since HT-2 toxin has two possible positions (at 3-OH and 4-OH) to be glusocylated. In addition, 15-monoacetoxyscirpenol-glucoside (MASGlc) was also detected in the identical sample.
Keywords: masked mycotoxin; mycotoxin glucoside; LC-Orbitrap MS; trichothecene; Fusarium; corn
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MDPI and ACS Style

Nakagawa, H.; Sakamoto, S.; Sago, Y.; Nagashima, H. Detection of Type A Trichothecene Di-Glucosides Produced in Corn by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry. Toxins 2013, 5, 590-604.

AMA Style

Nakagawa H, Sakamoto S, Sago Y, Nagashima H. Detection of Type A Trichothecene Di-Glucosides Produced in Corn by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry. Toxins. 2013; 5(3):590-604.

Chicago/Turabian Style

Nakagawa, Hiroyuki; Sakamoto, Shigeru; Sago, Yuki; Nagashima, Hitoshi. 2013. "Detection of Type A Trichothecene Di-Glucosides Produced in Corn by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry." Toxins 5, no. 3: 590-604.

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