Toxins 2013, 5(11), 2241-2257; doi:10.3390/toxins5112241
Article

Activation of RhoA,B,C by Yersinia Cytotoxic Necrotizing Factor (CNFy) Induces Apoptosis in LNCaP Prostate Cancer Cells

Received: 4 September 2013; in revised form: 1 November 2013 / Accepted: 5 November 2013 / Published: 21 November 2013
(This article belongs to the Special Issue Cytotoxic Necrotizing Factors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Prostate cancer is the most common malignancy, accounting for about 25% of all incident cases among men in industrialized countries. The human androgen-dependent prostate cancer cell line LNCaP, which is derived from a metastatic lesion of human prostatic adenocarcinoma, is frequently used to study prostate cancer associated signaling pathways in vitro. Recently it was described that Rho GTPase activation in these cells leads to apoptotic responses. We used the bacterial toxins CNFy and CNF1, which specifically and directly activate Rho GTPases by deamidation of a single glutamine. We asked whether these Rho activators could induce apoptosis in LNCaP cells. Our results indicate that RhoA activation, induced by CNFy, does lead to intrinsic apoptosis of the cells. Analysis of the underlying signaling pathway reveals that apoptosis induction requires the activity of Rho kinase (ROCK) and myosin activation, an apoptotic pathway previously identified in cancer stem cells.
Keywords: CNFy; apoptosis; prostate cancer; Rho-GTPases
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MDPI and ACS Style

Augspach, A.; List, J.H.; Wolf, P.; Bielek, H.; Schwan, C.; Elsässer-Beile, U.; Aktories, K.; Schmidt, G. Activation of RhoA,B,C by Yersinia Cytotoxic Necrotizing Factor (CNFy) Induces Apoptosis in LNCaP Prostate Cancer Cells. Toxins 2013, 5, 2241-2257.

AMA Style

Augspach A, List JH, Wolf P, Bielek H, Schwan C, Elsässer-Beile U, Aktories K, Schmidt G. Activation of RhoA,B,C by Yersinia Cytotoxic Necrotizing Factor (CNFy) Induces Apoptosis in LNCaP Prostate Cancer Cells. Toxins. 2013; 5(11):2241-2257.

Chicago/Turabian Style

Augspach, Anke; List, Joachim H.; Wolf, Philipp; Bielek, Heike; Schwan, Carsten; Elsässer-Beile, Ursula; Aktories, Klaus; Schmidt, Gudula. 2013. "Activation of RhoA,B,C by Yersinia Cytotoxic Necrotizing Factor (CNFy) Induces Apoptosis in LNCaP Prostate Cancer Cells." Toxins 5, no. 11: 2241-2257.

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