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Immunochemical Methods for Ochratoxin A Detection: A Review
ELTI Support VOF, Ambachtsweg 5, 6581 AX Malden, The Netherlands
Received: 28 February 2012; in revised form: 30 March 2012 / Accepted: 5 April 2012 / Published: 13 April 2012
Abstract: The safety of food and feed depends to a great deal on quality control. Numerous compounds and organisms may contaminate food and feed commodities and thus pose a health risk for consumers. The compound of interest in this review is ochratoxin A (OTA), a secondary metabolite of the fungi Aspergillus and Penicillium. Due to its adverse health effects, detection and quantification are of utmost importance. Quality control of food and feed requires extraction and analysis, including TLC, HPLC, MS, and immunochemical methods. Each of these methods has its advantages and disadvantages. However, with regard to costs and rapidity, immunochemical methods have gained much interest in the last decade. In this review an introduction to immunochemistry and assay design will be given to elucidate the principles. Further, the application of the various formats to the detection and quantification of ochratoxin will be described, including the use of commercially available kits.
Keywords: ochratoxin A (OTA); detection; quantification; immunochemical methods
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MDPI and ACS Style
Meulenberg, E.P. Immunochemical Methods for Ochratoxin A Detection: A Review. Toxins 2012, 4, 244-266.
Meulenberg EP. Immunochemical Methods for Ochratoxin A Detection: A Review. Toxins. 2012; 4(4):244-266.
Meulenberg, Eline P. 2012. "Immunochemical Methods for Ochratoxin A Detection: A Review." Toxins 4, no. 4: 244-266.