Next Article in Journal
Vixapatin (VP12), a C-Type Lectin-Protein from Vipera xantina palestinae Venom: Characterization as a Novel Anti-angiogenic Compound
Next Article in Special Issue
Immune Dysfunction in Uremia—An Update
Previous Article in Journal
Estimation of Multi-Mycotoxin Contamination in South African Compound Feeds
Previous Article in Special Issue
Protein-Bound Uremic Toxins: New Insight from Clinical Studies
Article Menu

Export Article

Open AccessArticle
Toxins 2012, 4(10), 849-861; doi:10.3390/toxins4100849

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

1
Department of Biomedical Engineering, Technomedicum, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia
2
Laboratory of Chemical Physics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia
3
Department of Nephrology UHL, County Council of Östergötland, Department of Medical Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden
*
Author to whom correspondence should be addressed.
Received: 30 June 2012 / Revised: 25 September 2012 / Accepted: 27 September 2012 / Published: 18 October 2012
(This article belongs to the Special Issue Uremic Toxins)
View Full-Text   |   Download PDF [768 KB, uploaded 18 October 2012]   |  

Abstract

The aim of this work was to evaluate the contributions of the main chromophores to the total UV absorbance of the spent dialysate and to assess removal dynamics of these solutes during optical on-line dialysis dose monitoring. High performance chromatography was used to separate and quantify UV-absorbing solutes in the spent dialysate sampled at the start and at the end of dialysis sessions. Chromatograms were monitored at 210, 254 and 280 nm routinely and full absorption spectra were registered between 200 and 400 nm. Nearly 95% of UV absorbance originates from solutes with high removal ratio, such as uric acid. The contributions of different solute groups vary at different wavelengths and there are dynamical changes in contributions during the single dialysis session. However, large standard deviation of the average contribution values within a series of sessions indicates remarkable differences between individual treatments. A noteworthy contribution of Paracetamol and its metabolites to the total UV absorbance was determined at all three wavelengths. Contribution of slowly dialyzed uremic solutes, such as indoxyl sulfate, was negligible. View Full-Text
Keywords: uremic toxins; hemodialysis; chromophores; retention solutes; absorption; ultraviolet-radiation; liquid-chromatography; dialysis dose; monitoring; spent dialysate uremic toxins; hemodialysis; chromophores; retention solutes; absorption; ultraviolet-radiation; liquid-chromatography; dialysis dose; monitoring; spent dialysate
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Arund, J.; Tanner, R.; Uhlin, F.; Fridolin, I. Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance? Toxins 2012, 4, 849-861.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top