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Toxins 2011, 3(7), 737-753; doi:10.3390/toxins3070737

Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies

1
Crop Protection and Management Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Tifton, GA 31794, USA
2
The J Craig Venter Institute, Rockville, MD 20850-3343, USA
3
Department of Plant Pathology, The University of Georgia, Tifton, GA 31793-0748, USA
4
Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
5
Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington, DC 20037, USA
6
Southern Regional Research Center, U.S. Department of Agriculture-Agricultural Research Service, New Orleans, LA 70124, USA
*
Author to whom correspondence should be addressed.
Received: 27 April 2011 / Revised: 9 June 2011 / Accepted: 14 June 2011 / Published: 24 June 2011
(This article belongs to the Special Issue Aflatoxins 2011)
View Full-Text   |   Download PDF [392 KB, uploaded 24 June 2011]

Abstract

Aspergillus flavus and A. parasiticus infect peanut seeds and produce aflatoxins, which are associated with various diseases in domestic animals and humans throughout the world. The most cost-effective strategy to minimize aflatoxin contamination involves the development of peanut cultivars that are resistant to fungal infection and/or aflatoxin production. To identify peanut Aspergillus-interactive and peanut Aspergillus-resistance genes, we carried out a large scale peanut Expressed Sequence Tag (EST) project which we used to construct a peanut glass slide oligonucleotide microarray. The fabricated microarray represents over 40% of the protein coding genes in the peanut genome. For expression profiling, resistant and susceptible peanut cultivars were infected with a mixture of Aspergillus flavus and parasiticus spores. The subsequent microarray analysis identified 62 genes in resistant cultivars that were up-expressed in response to Aspergillus infection. In addition, we identified 22 putative Aspergillus-resistance genes that were constitutively up-expressed in the resistant cultivar in comparison to the susceptible cultivar. Some of these genes were homologous to peanut, corn, and soybean genes that were previously shown to confer resistance to fungal infection. This study is a first step towards a comprehensive genome-scale platform for developing Aspergillus-resistant peanut cultivars through targeted marker-assisted breeding and genetic engineering. View Full-Text
Keywords: EST; microarray; gene profiling; peanut-fungus interaction; resistance genes; Aspergillus flavus; A. parasiticus; metarep EST; microarray; gene profiling; peanut-fungus interaction; resistance genes; Aspergillus flavus; A. parasiticus; metarep
EST; microarray; gene profiling; peanut-fungus interaction; resistance genes; Aspergillus flavus; A. parasiticus; metarep EST; microarray; gene profiling; peanut-fungus interaction; resistance genes; Aspergillus flavus; A. parasiticus; metarep
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Guo, B.; Fedorova, N.D.; Chen, X.; Wan, C.-H.; Wang, W.; Nierman, W.C.; Bhatnagar, D.; Yu, J. Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies. Toxins 2011, 3, 737-753.

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