Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Toxins 2011, 3(6), 640-646; doi:10.3390/toxins3060640
Brief Report

Detection of stx1 and stx2 Genes in Pennsylvanian White-Tailed Deer

1
, 2
 and 2,*
Received: 3 May 2011; in revised form: 10 June 2011 / Accepted: 14 June 2011 / Published: 16 June 2011
(This article belongs to the Special Issue Shiga Toxin)
View Full-Text   |   Download PDF [219 KB, uploaded 16 June 2011]
Abstract: Shiga toxin-producing E. coli carrying the stx1 and/or stx2 genes can cause multi-symptomatic illness in humans. A variety of terrestrial and aquatic environmental reservoirs of stx have been described. Culture based detection of microbes in deer species have found a low percentage of samples that have tested positive for Stx-producing microbes, suggesting that while deer may contain these microbes, their overall abundance in deer is low. In this study, quantitative PCR (qPCR) was utilized to test for the presence of stx genes in white-tailed deer fecal matter in western Pennsylvania. In this culture independent screening, nearly half of the samples tested positive for the stx2 gene, with a bias towards samples that were concentrated with stx2. This study, while limited in scope, suggests that deer may be a greater reservoir for stx than was previously thought.
Keywords: Shiga toxin; white-tailed deer; quantitative PCR Shiga toxin; white-tailed deer; quantitative PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Kistler, W.M.; Mulugeta, S.; Mauro, S.A. Detection of stx1 and stx2 Genes in Pennsylvanian White-Tailed Deer. Toxins 2011, 3, 640-646.

AMA Style

Kistler WM, Mulugeta S, Mauro SA. Detection of stx1 and stx2 Genes in Pennsylvanian White-Tailed Deer. Toxins. 2011; 3(6):640-646.

Chicago/Turabian Style

Kistler, Whitney M.; Mulugeta, Surafel; Mauro, Steven A. 2011. "Detection of stx1 and stx2 Genes in Pennsylvanian White-Tailed Deer." Toxins 3, no. 6: 640-646.


Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert