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Detection of Fumonisin B1 and Ochratoxin A in Grain Products Using Microsphere-Based Fluid Array Immunoassays
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington DC, 20375, USA
* Author to whom correspondence should be addressed.
Received: 5 January 2010; in revised form: 17 February 2010 / Accepted: 23 February 2010 / Published: 25 February 2010
Abstract: Grain products are a staple of diets worldwide and therefore, the ability to accurately and efficiently detect foodborne contaminants such as mycotoxins is of importance to everyone. Here we describe an indirect competitive fluid array fluoroimmunoassay to quantify the mycotoxins, fumonisin B1 and ochratoxin A. Both toxins were immobilized to the surface of microspheres using a variety of intermediate molecules and binding of biotinylated "tracer" antibody tracers determined through flow cytometry using streptavidin-phycoerythrin conjugates and the Luminex100 flow cytometer. Competitive assays were developed where the binding of biotinylated monoclonal antibodies to fumonisin B and ochratoxin A was competitively inhibited by different concentrations of those toxins in solution. Concentrations of fumonisin giving 50% inhibition were 300 pg/mL in buffer, 100 ng/g in spiked oats, and 1 μg/g in spiked cornmeal; analogous concentrations for ochratoxin A were 30 ng/mL in buffer, 30 ng/g in spiked oats, and 10 ng/g in spiked corn. The future challenge will be to expand the number of mycotoxins tested both individually and in multiplexed format using this platform.
Keywords: mycotoxin; immunoassay; bead array; fumonisin; ochratoxin; detection
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Anderson, G.P.; Kowtha, V.A.; Taitt, C.R. Detection of Fumonisin B1 and Ochratoxin A in Grain Products Using Microsphere-Based Fluid Array Immunoassays. Toxins 2010, 2, 297-309.
Anderson GP, Kowtha VA, Taitt CR. Detection of Fumonisin B1 and Ochratoxin A in Grain Products Using Microsphere-Based Fluid Array Immunoassays. Toxins. 2010; 2(2):297-309.
Anderson, George P.; Kowtha, Vasudha A.; Taitt, Chris R. 2010. "Detection of Fumonisin B1 and Ochratoxin A in Grain Products Using Microsphere-Based Fluid Array Immunoassays." Toxins 2, no. 2: 297-309.