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Toxins 2018, 10(6), 223; https://doi.org/10.3390/toxins10060223

Fructo-Oligosaccharide (DFA III) Feed Supplementation for Mitigation of Mycotoxin Exposure in Cattle—Clinical Evaluation by a Urinary Zearalenone Monitoring System

1
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
2
Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan
3
Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan
4
Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro 080-0835, Japan
5
Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan
6
Soo Agricultural Mutual Aid Association, Kagoshima 890-8212, Japan
7
Department of Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka
8
Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, The Netherlands
9
Laboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan
*
Author to whom correspondence should be addressed.
Received: 1 May 2018 / Revised: 23 May 2018 / Accepted: 30 May 2018 / Published: 1 June 2018
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Abstract

The potential effect of difructose anhydride III (DFA III) supplementation in cattle feed was evaluated using a previously developed urinary-zearalenone (ZEN) monitoring system. Japanese Black cattle from two beef herds aged 9–10 months were used. DFA III was supplemented for two weeks. ZEN concentrations in feed were similar in both herds (0.27 and 0.22 mg/kg in roughage and concentrates, respectively), and below the maximum allowance in Japan. ZEN, α-zearalenol (α-ZOL), and β-ZOL concentrations in urine were measured using LC/MS/MS the day before DFA III administration, 9 and 14 days thereafter, and 9 days after supplementation ceased. Significant differences in ZEN, α-ZOL, β-ZOL, and total ZEN were recorded on different sampling dates. The concentration of inorganic phosphate in DFA III-supplemented animals was significantly higher than in controls on day 23 (8.4 vs. 7.7 mg/dL), suggesting a possible role of DFA III in tight junction of intestinal epithelial cells. This is the first evidence that DFA III reduces mycotoxin levels reaching the systemic circulation and excreted in urine. This preventive effect may involve an improved tight-junction-dependent intestinal barrier function. Additionally, our practical approach confirmed that monitoring of urinary mycotoxin is useful for evaluating the effects of dietary supplements to prevent mycotoxin adsorption. View Full-Text
Keywords: cattle; DFA III; mycotoxin; tight-junction; urine; blood cattle; DFA III; mycotoxin; tight-junction; urine; blood
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Toda, K.; Uno, S.; Kokushi, E.; Shiiba, A.; Hasunuma, H.; Matsumoto, D.; Ohtani, M.; Yamato, O.; Shinya, U.; Wijayagunawardane, M.; Fink-Gremmels, J.; Taniguchi, M.; Takagi, M. Fructo-Oligosaccharide (DFA III) Feed Supplementation for Mitigation of Mycotoxin Exposure in Cattle—Clinical Evaluation by a Urinary Zearalenone Monitoring System. Toxins 2018, 10, 223.

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