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Toxins 2018, 10(5), 177; https://doi.org/10.3390/toxins10050177

Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition

1
Laboratory of Toxinology, Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo—USP, Ribeirão Preto, SP 14040-903, Brazil
2
GIGA Proteomics Facility, GIGA Institute, University of Liège, 4000 Liège, Belgium
3
Laboratory of Mass Spectrometry, Department of Chemistry, University of Liège, 4000 Liège, Belgium
Current address: Fernanda Gobbi Amorim and Suely Vilela Sampaio, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo—USP, Av. do Café s/no. Campus Universitário, Ribeirão Preto, SP 14040-903, Brazil.
*
Authors to whom correspondence should be addressed.
Received: 11 April 2018 / Revised: 22 April 2018 / Accepted: 23 April 2018 / Published: 26 April 2018
(This article belongs to the Section Animal Venoms)
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Abstract

Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes’ age, habitat, diet, and sexual dimorphism. The present study reports the first in-depth investigation of venom composition in male and female Bothrops moojeni (B. moojeni) snakes (BmooM and BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, and immunoreactivity characterization. Compared with BmooM venom, BmooF venom exhibited weaker hyaluronidase, metalloproteinase, and phospholipase activity; stronger recognition by anti-bothropic serum; 1.4-fold stronger cytotoxicity; and greater number of peptides. The increased L-amino acid oxidase expression probably accounted for the stronger immunoreactivity and cytotoxicity of BmooF venom. BmooF and BmooM venom shared only 19% peptides. Some venom components were gender-specific, such as phospholipases B, phospholipase inhibitor, and hyaluronidases in BmooM, and cysteine-rich secretory proteins in BmooF. In conclusion, we describe herein the first proteomics study of B. moojeni snake venom and an in-depth characterization of gender-specific differences in venom composition. Altogether, our findings not only stress the importance of considering the snake’s gender during antivenom production, but also help to identify new potential drugs and biotechnological tools. View Full-Text
Keywords: proteome; Bothrops moojeni; toxins; venomics proteome; Bothrops moojeni; toxins; venomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Amorim, F.G.; Costa, T.R.; Baiwir, D.; De Pauw, E.; Quinton, L.; Sampaio, S.V. Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition. Toxins 2018, 10, 177.

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